Substrate Specificity and Regioselectivity of Δ12 and ω3 Fatty Acid Desaturases fromSaccharomyces kluyveri

2008 ◽  
Vol 72 (12) ◽  
pp. 3174-3179 ◽  
Author(s):  
Takahiro OURA ◽  
Susumu KAJIWARA
Keyword(s):  
Fatty Acid ◽  
Substrate Specificity ◽  
Fatty Acid Desaturases

scholarly journals Plastidic Δ6 Fatty-Acid Desaturases with Distinctive Substrate Specificity Regulate the Pool of C18-PUFAs in the Ancestral Picoalga Ostreococcus tauri

2020 ◽  
Vol 184 (1) ◽  
pp. 82-96
Author(s):  
Charlotte Degraeve-Guilbault ◽  
Rodrigo E. Gomez ◽  
Cécile Lemoigne ◽  
Nattiwong Pankansem ◽  
Soizic Morin ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Substrate Specificity ◽  
Fatty Acid Desaturases ◽  
Ostreococcus Tauri

scholarly journals Identification of amino acid residues that determine the substrate specificity of mammalian membrane-bound front-end fatty acid desaturases

2015 ◽  
Vol 57 (1) ◽  
pp. 89-99 ◽  
Author(s):  
Kenshi Watanabe ◽  
Makoto Ohno ◽  
Masahiro Taguchi ◽  
Seiji Kawamoto ◽  
Kazuhisa Ono ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Amino Acid ◽  
Substrate Specificity ◽  
Amino Acid Residues ◽  
Fatty Acid Desaturases ◽  
Front End ◽  
Membrane Bound

Presence or absence? Primary structure, regioselectivity and evolution of Δ12/ω3 fatty acid desaturases in nematodes

2019 ◽  
Vol 1864 (9) ◽  
pp. 1194-1205 ◽  
Author(s):  
Ralph Menzel ◽  
Henrik von Chrzanowski ◽  
Tina Tonat ◽  
Kristina van Riswyck ◽  
Patrick Schliesser ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Primary Structure ◽  
Fatty Acid Desaturases

ChemInform Abstract: Synthesis of Inhibitors of Fatty Acid Desaturases

ChemInform ◽  
2010 ◽  
Vol 28 (13) ◽  
pp. no-no
Author(s):  
J. R. AL DULAYYMI ◽  
M. S. BAIRD ◽  
C. M. DALE ◽  
B. GREHAN ◽  
M. F. SHORTT ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Fatty Acid Desaturases

Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Fatty Acid Desaturases ◽  
Desaturase Gene ◽  
Saccharomyces Kluyveri ◽  
Fatty Acid Desaturase Gene

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.

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