scholarly journals In VitroAugmentation of Natural Killer Activity and Interferon-γ Production in Murine Spleen Cells withAgaricus blazeiFruiting Body Fractions

2005 ◽  
Vol 69 (12) ◽  
pp. 2466-2469 ◽  
Author(s):  
Ming ZHONG ◽  
Akihiro TAI ◽  
Itaru YAMAMOTO
Keyword(s):  
Natural Killer ◽  
Natural Killer Activity ◽  
Interferon Γ ◽  
Spleen Cells ◽  
Killer Activity ◽  
Murine Spleen

scholarly journals Cloned cell lines with natural killer activity. Specificity, function, and cell surface markers.

1981 ◽  
Vol 153 (3) ◽  
pp. 545-556 ◽  
Author(s):  
G Dennert ◽  
G Yogeeswaran ◽  
S Yamagata
Keyword(s):  
Cell Surface ◽  
Nk Cells ◽  
Natural Killer ◽  
Cell Lines ◽  
Nk Cell ◽  
Cell Lineage ◽  
Natural Killer Activity ◽  
Spleen Cells ◽  
Con A ◽  
Killer Activity

Cell lines with natural killer (NK) activity grown from native spleen cells cultured in medium conditioned by spleen cells proliferating in the presence of concanavalin A (Con A) were characterized. One NK cell line was cloned and assayed on several human and mouse NK-sensitive targets to analyze whether target specificities segregate upon cloning. Results showed that NK clones display target specificities identical to NK cells in normal spleen. This suggests that NK cells have no clonally distributed specific receptors to a given target. They may, however, have receptors which recognize identical antigens on all NK-sensitive targets or may possess multiple receptors for different target specificities. NK lines could not be demonstrated to possess activity in antibody-dependent cell-mediated cytotoxicity, nor did they effect mutual lysis. In the presence of Con A, NK cells exhibited dramatically enhanced lysis of NK-sensitive targets but only a slight increase in lysis of NK-insensitive targets. This indicates that the degree of lysis of an NK target is a function of two variables: effector binding to the target and target sensitivity to lysis. Furthermore, it suggests that the affinity of the putative antigen receptors on NK effectors must be rather weak. Cell surface marker analysis reveals that NK cell lines are Thy 1.2+, Lt-1-2-, T200+, asialo GM1+, and asialo GM2+. These markers distinguish NK cells from cytolytic thymus-derived lymphocytes, without resolving the question of classification within a give hematopoietic cell lineage.

scholarly journals Effect of benzene and its metabolites on natural killer activity of mouse spleen cells in vitro.

Sangyo Igaku ◽  
1989 ◽  
Vol 31 (5) ◽  
pp. 330-334
Author(s):  
Xiao-hui FAN ◽  
Yoko HIRATA ◽  
Masayasu MINAMI
Keyword(s):  
Natural Killer ◽  
Natural Killer Activity ◽  
Mouse Spleen ◽  
Spleen Cells ◽  
Killer Activity

scholarly journals Natural killer activity of the spleen cells of Ehrlich tumor-bearing mice treated with Copaifera multijuga extract

2019 ◽  
Vol 29 (1) ◽  
pp. 32408
Author(s):  
Paloma Filomena Gouveia Rodrigues ◽  
Lucinéia Reuse Albiero ◽  
Eduardo Figueredo Nery ◽  
Taiany Oliveira Kelly ◽  
Jeniffer Charlene Silva Dalazen ◽  
...  
Keyword(s):  
Natural Killer ◽  
Saline Solution ◽  
Ethanolic Extract ◽  
Natural Killer Activity ◽  
Control Group ◽  
Spleen Cells ◽  
Ehrlich Tumor ◽  
Killer Activity ◽  
Tumor Bearing ◽  
Phosphate Buffered Saline

AIMS: Copaifera multijuga Hayne oleoresin is commonly used in traditional medicine owing to its anti-inflammatory, antiseptic, antitumor, and antibacterial properties. However, little is known about the effect of the compounds from the bark of this plant. In this study, the immunomodulatory effect of the ethanolic extract of C. multijuga bark via natural killer activity of non-adherent spleen cells of Ehrlich tumor-bearing mice was evaluated.METHODS: Male Swiss mice were inoculated subcutaneously with 1×106 Ehrlich tumor cells (Ehrlich and Ehrlich/C. multijuga group) or phosphate buffered saline solution (control group and C. multijuga group) and treated orally daily with C. multijuga extract (200 mg kg-1, 0.1 mL per mouse, for the Ehrlich/C. multijuga and C. multijuga groups) or phosphate buffered saline solution (control group and Ehrlich group). The four experimental groups consisted in eight mice each and were organized in two sets, one treated for seven days and another treated for 14 days, totalizing 64 mice throughout the experiment. Twenty-four hours after the last oral administration, the mice were euthanized and the spleen tissue was isolated to prepare a non-adherent spleen cell suspension and to evaluate natural killer activity. Data are presented as the cell lysis percentage of Yac.1 target cells by non-adherent spleen cells.RESULTS: Treatment for seven days increased natural killer activity in the Ehrlich/C. multijuga group (21.20±8.89, p<0.05) compared to the control group (3.14±2.71, p<0.05); however, this effect was not maintained in the groups treated for 14 days (Control: 6.02±6.98, Ehrlich: 4.82±7.72, C. multijuga: 2.07±2.10, Ehrlich/C. multijuga: 2.01±1.63, p>0.05).CONCLUSIONS: Treatment for seven days with an ethanolic extract of C. multijuga bark enhanced the natural killer activity of non-adherent spleen cells from Ehrlich tumor-bearing mice.

scholarly journals Augmentation of Killer Activity of Murine Spleen Cells against Allogeneic and Syngeneic Tumor Cells by Inoculation of Alloantigenin vivo

1987 ◽  
Vol 31 (12) ◽  
pp. 1245-1254 ◽  
Author(s):  
Yasuaki Nishi ◽  
Tomohide Hosokawa ◽  
Akira Aoike ◽  
Takeshi Kamahora ◽  
Hiromasa Sudo ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Spleen Cells ◽  
Killer Activity ◽  
Syngeneic Tumor ◽  
Murine Spleen

scholarly journals Kinetics of in vitro natural killer activity against K562 cells as detected by flow cytometry

Cytometry ◽  
1998 ◽  
Vol 32 (4) ◽  
pp. 280-285 ◽  
Author(s):  
Loris Zamai ◽  
Adriana R. Mariani ◽  
Giorgio Zauli ◽  
Luigi Rodella ◽  
Rita Rezzani ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Natural Killer ◽  
Natural Killer Activity ◽  
K562 Cells ◽  
Killer Activity ◽  
Kinetics Of

Effect of serum factor(s) of endometriosis patients on natural killer activity

1989 ◽  
Vol 15 ◽  
pp. 194
Author(s):  
H.S. Wang ◽  
M. Kariya ◽  
H. Kanzaki ◽  
M. Yoshida ◽  
M. Tokushige ◽  
...  
Keyword(s):  
Natural Killer ◽  
Natural Killer Activity ◽  
Serum Factor ◽  
Killer Activity
Sign in / Sign up

Export Citation Format

Share Document