Yeast Δ12 Fatty Acid Desaturase: Gene Cloning, Expression, and Function

2004 ◽  
Vol 68 (3) ◽  
pp. 721-727 ◽  
Author(s):  
Kyoko WATANABE ◽  
Takahiro OURA ◽  
Hiromichi SAKAI ◽  
Susumu KAJIWARA
Keyword(s):  
Fatty Acid ◽  
Gene Cloning ◽  
Fatty Acid Desaturase ◽  
Desaturase Gene ◽  
And Function ◽  
Fatty Acid Desaturase Gene

A modified n–3 fatty acid desaturase gene from Caenorhabditis briggsae produced high proportion of DHA and DPA in transgenic mice

2008 ◽  
Vol 17 (4) ◽  
pp. 717-725 ◽  
Author(s):  
Guiming Zhu ◽  
Hongxing Chen ◽  
Xiaojie Wu ◽  
Yanrong Zhou ◽  
Jianshen Lu ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Transgenic Mice ◽  
Fatty Acid Desaturase ◽  
Caenorhabditis Briggsae ◽  
Desaturase Gene ◽  
Fatty Acid Desaturase Gene

Saccharomyces kluyveri FAD3 encodes an ω3 fatty acid desaturase

Microbiology ◽  
2004 ◽  
Vol 150 (6) ◽  
pp. 1983-1990 ◽  
Author(s):  
Takahiro Oura ◽  
Susumu Kajiwara
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Linoleic Acid ◽  
Linolenic Acid ◽  
Functional Characterization ◽  
Fatty Acid Desaturase ◽  
Fatty Acid Desaturases ◽  
Desaturase Gene ◽  
Saccharomyces Kluyveri ◽  
Fatty Acid Desaturase Gene

Fungi, like plants, are capable of producing the 18-carbon polyunsaturated fatty acids linoleic acid and α-linolenic acid. These fatty acids are synthesized by catalytic reactions of Δ12 and ω3 fatty acid desaturases. This paper describes the first cloning and functional characterization of a yeast ω3 fatty acid desaturase gene. The deduced protein encoded by the Saccharomyces kluyveri FAD3 gene (Sk-FAD3) consists of 419 amino acids, and shows 30–60 % identity with Δ12 fatty acid desaturases of several eukaryotic organisms and 29–31 % identity with ω3 fatty acid desaturases of animals and plants. During Sk-FAD3 expression in Saccharomyces cerevisiae, α-linolenic acid accumulated only when linoleic acid was added to the culture medium. The disruption of Sk-FAD3 led to the disappearance of α-linolenic acid in S. kluyveri. These findings suggest that Sk-FAD3 is the only ω3 fatty acid desaturase gene in this yeast. Furthermore, transcriptional expression of Sk-FAD3 appears to be regulated by low-temperature stress in a manner different from the other fatty acid desaturase genes in S. kluyveri.

scholarly journals Association of Polymorphism in Fatty Acid Desaturase Gene with the Risk of Type 2 Diabetes in Iranian Population

2018 ◽  
Vol 7 (1) ◽  
pp. 98 ◽  
Author(s):  
Vahid Mansouri ◽  
Shaghayegh Haghjooy Javanmard ◽  
Manijeh Mahdavi ◽  
MohammadHasan Tajedini
Keyword(s):  
Type 2 Diabetes ◽  
Fatty Acid ◽  
Fatty Acid Desaturase ◽  
Iranian Population ◽  
Desaturase Gene ◽  
Fatty Acid Desaturase Gene
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