Molecular Cloning and Expression of the Gene Encoding Family 19 Chitinase fromStreptomycessp. J-13-3

2004 ◽  
Vol 68 (2) ◽  
pp. 341-351 ◽  
Author(s):  
Katsuichiro OKAZAKI ◽  
Yousuke YAMASHITA ◽  
Minoru NODA ◽  
Noriyuki SUEYOSHI ◽  
Isamu KAMESHITA ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Cloning And Expression ◽  
Gene Encoding ◽  
J 13 ◽  
Family 19 Chitinase

Molecular cloning and expression of the gene encoding ADP-glucose pyrophosphorylase from the cyanobacteriumAnabaena sp. strain PCC 7120

1992 ◽  
Vol 20 (1) ◽  
pp. 37-47 ◽  
Author(s):  
Yee-yung Charng ◽  
Genichi Kakefuda ◽  
Alberto A. Iglesias ◽  
William J. Buikema ◽  
Jack Preiss
Keyword(s):  
Molecular Cloning ◽  
Cloning And Expression ◽  
Gene Encoding ◽  
Adp Glucose Pyrophosphorylase ◽  
Pcc 7120

Molecular Cloning and Expression of β-1,4-endoxylanase Gene from Chaetomium cupreum in Yeast Saccharomyces cerevisiae

2013 ◽  
Vol 779-780 ◽  
pp. 191-194
Author(s):  
Hai Yan Zhang ◽  
Wen Rao Li ◽  
Min Li
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Nucleotide Sequence ◽  
Heterologous Expression ◽  
Molecular Mass ◽  
Molecular Cloning ◽  
Cloning And Expression ◽  
Cdna Fragment ◽  
Gene Encoding ◽  
Calculated Molecular Mass

The gene encoding an endo-β-1,4-xylanase (XynCC) fromchaetomium cupreumwas amplified using PCR. The nucleotide sequence of a 690 bp cDNA fragment was determined. Based on the nucleotide sequence, calculated molecular mass of the enzyme was 24.7 kDa. The XynCC gene was inserted into the pYES2 vector and transferred into the cells ofS. cerevisiaeH158 for heterologous expression.

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