Molecular cloning and expression of the hot pepper ERabp1 gene encoding auxin-binding protein

1996 ◽  
Vol 32 (5) ◽  
pp. 995-997 ◽  
Author(s):  
Soon-Yong Choi
Keyword(s):  
Molecular Cloning ◽  
Binding Protein ◽  
Cloning And Expression ◽  
Hot Pepper ◽  
Gene Encoding ◽  
Auxin Binding ◽  
Auxin Binding Protein

Cloning and characterization of a gene encoding ABP57, a soluble auxin-binding protein

2009 ◽  
Vol 3 (4) ◽  
pp. 293-299 ◽  
Author(s):  
Keunpyo Lee ◽  
Myung-Il Kim ◽  
Yu-Jihn Kwon ◽  
Minkyun Kim ◽  
Yong-Sam Kim ◽  
...  
Keyword(s):  
Binding Protein ◽  
Gene Encoding ◽  
Auxin Binding ◽  
Auxin Binding Protein

scholarly journals A Soluble Auxin-Binding Protein from Hyoscyamus muticus Is a Glutathione S-Transferase

1993 ◽  
Vol 102 (1) ◽  
pp. 29-34 ◽  
Author(s):  
J. Bilang ◽  
H. Macdonald ◽  
P. J. King ◽  
A. Sturm
Keyword(s):  
Binding Protein ◽  
Hyoscyamus Muticus ◽  
Glutathione S Transferase ◽  
Auxin Binding ◽  
Auxin Binding Protein

scholarly journals In silico promoter and expression analyses of rice Auxin Binding Protein 57 (ABP57)

2021 ◽  
Vol 8 (3) ◽  
pp. 741-748
Author(s):  
Farah Afiqah Baharuddin ◽  
Zhan Xuan Khong ◽  
Zamri Zainal ◽  
Noor Liyana Sukiran
Keyword(s):  
Abiotic Stress ◽  
In Silico ◽  
Binding Protein ◽  
Transcript Level ◽  
Regulatory Elements ◽  
Biochemical Properties ◽  
Auxin Receptor ◽  
Reverse Transcription Pcr ◽  
Auxin Binding ◽  
Auxin Binding Protein

Auxin Binding Protein 57 (ABP57) is one of the molecular components involved in rice response to abiotic stress. The ABP57 gene encodes an auxin receptor which functions in activating the plasma membrane H+-ATPase. Biochemical properties of ABP57 have been characterized; however, the function of ABP57, particularly on stress and hormone responses is still limited. This study was conducted to understand the regulation of ABP57 expression under abiotic stress. Thus, in silico identification of cis-acting regulatory elements (CAREs) in the promoter region of ABP57 was performed. Several motifs and transcription factor binding site (TFBS) that are involved in abiotic stress such as ABRE, DRE, AP2/EREBP, WRKY and NAC were identified. Next, expression analysis of ABP57 under drought, salt, auxin (IAA) and abscisic acid (ABA) was conducted by reverse transcription-PCR (RT-PCR) to verify the effect of these treatments on ABP57 transcript level. ABP57 was expressed at different levels in the shoot and root under drought conditions, and its expression was increased under IAA and ABA treatments. Moreover, our results showed that ABP57 expression in the root was more responsive to drought, auxin and ABA treatments compared to its transcript in the shoot. This finding suggests that ABP57 is a drought-responsive gene and possibly regulated by IAA and ABA.

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