scholarly journals Biphenyl-inducible Promoters in a Polychlorinated Biphenyl-degrading Bacterium,Rhodococcussp. RHA1

2004 ◽  
Vol 68 (6) ◽  
pp. 1249-1258 ◽  
Author(s):  
Hisashi TAKEDA ◽  
Naho HARA ◽  
Masayuki SAKAI ◽  
Akihiro YAMADA ◽  
Keisuke MIYAUCHI ◽  
...  
Keyword(s):  
Polychlorinated Biphenyl ◽  
Inducible Promoters ◽  
Degrading Bacterium

Rhodococcus biphenylivorans sp. nov., a polychlorinated biphenyl-degrading bacterium

2014 ◽  
Vol 107 (1) ◽  
pp. 55-63 ◽  
Author(s):  
Xiaomei Su ◽  
Yindong Liu ◽  
Muhammad Zaffar Hashmi ◽  
Jinxing Hu ◽  
Linxian Ding ◽  
...  
Keyword(s):  
Polychlorinated Biphenyl ◽  
Degrading Bacterium

scholarly journals Complete Genome Sequence of the Polychlorinated Biphenyl-Degrading Bacterium Pseudomonas putida KF715 (NBRC 110667) Isolated from Biphenyl-Contaminated Soil

2017 ◽  
Vol 5 (7) ◽  
Author(s):  
Hikaru Suenaga ◽  
Atsushi Yamazoe ◽  
Akira Hosoyama ◽  
Nobutada Kimura ◽  
Jun Hirose ◽  
...  
Keyword(s):  
Pseudomonas Putida ◽  
Genome Sequence ◽  
Complete Genome Sequence ◽  
Complete Genome ◽  
Polychlorinated Biphenyl ◽  
Sole Source ◽  
Circular Chromosome ◽  
Content Type ◽  
Degrading Bacterium ◽  
Catabolic Genes

ABSTRACT Pseudomonas putida KF715 (NBRC 110667) utilizes biphenyl as a sole source of carbon and degrades polychlorinated biphenyls (PCBs). Here, we report a complete genome sequence of the KF715 strain, which comprises a circular chromosome and four plasmids. Biphenyl catabolic genes were located on the largest plasmid, pKF715A.

scholarly journals Rapid Assessment of the Physiological Status of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102 by Flow Cytometry

2002 ◽  
Vol 68 (4) ◽  
pp. 2031-2035 ◽  
Author(s):  
Yoshinori Hiraoka ◽  
Kazuhide Kimbara
Keyword(s):  
Flow Cytometry ◽  
Polychlorinated Biphenyl ◽  
Rapid Assessment ◽  
Live Cells ◽  
Physiological Status ◽  
Comamonas Testosteroni ◽  
Double Staining ◽  
Permeabilized Cells ◽  
Degrading Bacterium ◽  
Double Staining Method

ABSTRACT The viability of the polychlorinated biphenyl-degrading bacterium Comamonas testosteroni TK102 was assessed by flow cytometry (FCM) with the fluorogenic ester Calcein-AM (CAM) and the nucleic acid dye propidium iodide (PI). CAM stained live cells, whereas PI stained dead cells. When double staining with CAM and PI was performed, three physiological states, i.e., live (calcein positive, PI negative), dead (calcein negative, PI positive), and permeabilized (calcein positive, PI positive), were detected. To evaluate the reliability of this double-staining method, suspensions of live and dead cells were mixed in various proportions and analyzed by FCM. The proportion of dead cells measured by FCM directly correlated with the proportion of dead cells in the sample (y = 0.9872 x + 0.18; R 2 = 0.9971). In addition, the proportion of live cells measured by FCM inversely correlated with the proportion of dead cells in the sample (y = −0.9776 x + 98.36; R 2 = 0.9962). The proportion of permeabilized cells was consistently less than 2%. These results indicate that FCM in combination with CAM and PI staining is rapid (≤1 h) and distinguishes correctly among live, dead, and permeabilized cells.

scholarly journals Flow Cytometry Analysis of Changes in the DNA Content of the Polychlorinated Biphenyl Degrader Comamonas testosteroni TK102: Effect of Metabolites on Cell-Cell Separation

2002 ◽  
Vol 68 (10) ◽  
pp. 5104-5112 ◽  
Author(s):  
Yoshinori Hiraoka ◽  
Tohru Yamada ◽  
Keiko Tone ◽  
Yutaka Futaesaku ◽  
Kazuhide Kimbara
Keyword(s):  
Flow Cytometry ◽  
Dna Content ◽  
Cell Separation ◽  
Polychlorinated Biphenyl ◽  
Gene Replacement ◽  
Comamonas Testosteroni ◽  
Single Chromosome ◽  
Degrading Bacterium ◽  
Intermediate Metabolites ◽  
Cell Cell

ABSTRACT Flow cytometry was used to monitor changes in the DNA content of the polychlorinated biphenyl (PCB)-degrading bacterium Comamonas testosteroni TK102 during growth in the presence or absence of PCBs. In culture medium without PCBs, the majority of stationary-phase cells contained a single chromosome. In the presence of PCBs, the percentage of cells containing two chromosomes increased from 12% to approximately 50%. In contrast, addition of PCBs did not change the DNA contents of three species that are unable to degrade PCBs. In addition, highly chlorinated PCBs that are not degraded by TK102 did not result in a change in the DNA content. These results suggest that PCBs did not affect the DNA content of the cells directly; rather, the intermediate metabolites resulting from the degradation of PCBs caused the increase in DNA content. To study the effect of intermediate metabolites on the DNA content of the cells, four bph genes, bphA1, bphB, bphC, and bphD, were disrupted by gene replacement. The resulting mutant strains accumulated intermediate metabolites when they were grown in the presence of PCBs or biphenyl (BP). When the bphB gene was disrupted, the percentage of cells containing two chromosomes increased in cultures grown with PCBs or BP. When grown with BP, cultures of this mutant accumulated two intermediate metabolites, 2-hydroxybiphenyl (2-OHBP) and 3-OHBP. Addition of 2- or 3-OHBP to a wild-type TK102 and non-PCB-degrading species culture also resulted in an increase in the percentage of cells containing two chromosomes. Electron microscopy revealed that cell-cell separation was inhibited in this culture. This is the first report that hydroxy-BPs can inhibit bacterial cell separation while allowing continued DNA replication.

A Gram-positive Polychlorinated Biphenyl-degrading Bacterium,Rhodococcus erythropolisStrain TA421, Isolated from a Termite Ecosystem

1994 ◽  
Vol 58 (11) ◽  
pp. 2111-2113 ◽  
Author(s):  
Seon-Yong Chung ◽  
Michihisa Maeda ◽  
Eun Song ◽  
Koki Horikoshij ◽  
Toshiaki Kudo
Keyword(s):  
Polychlorinated Biphenyl ◽  
Gram Positive ◽  
Degrading Bacterium

scholarly journals Growth Substrate- and Phase-Specific Expression of Biphenyl, Benzoate, and C1 Metabolic Pathways in Burkholderia xenovorans LB400

2005 ◽  
Vol 187 (23) ◽  
pp. 7996-8005 ◽  
Author(s):  
V. J. Denef ◽  
M. A. Patrauchan ◽  
C. Florizone ◽  
J. Park ◽  
T. V. Tsoi ◽  
...  
Keyword(s):  
Polychlorinated Biphenyl ◽  
Transition Phase ◽  
Growth Substrate ◽  
Specific Expression ◽  
General Stress Response ◽  
Burkholderia Xenovorans ◽  
Degrading Bacterium ◽  
Burkholderia Xenovorans Lb400 ◽  
Regulated Expression ◽  
Order Of Magnitude

ABSTRACT Recent microarray experiments suggested that Burkholderia xenovorans LB400, a potent polychlorinated biphenyl (PCB)-degrading bacterium, utilizes up to three apparently redundant benzoate pathways and a C1 metabolic pathway during biphenyl and benzoate metabolism. To better characterize the roles of these pathways, we performed quantitative proteome profiling of cells grown on succinate, benzoate, or biphenyl and harvested during either mid-logarithmic growth or the transition between the logarithmic and stationary growth phases. The Bph enzymes, catabolizing biphenyl, were ∼16-fold more abundant in biphenyl- versus succinate-grown cells. Moreover, the upper and lower bph pathways were independently regulated. Expression of each benzoate pathway depended on growth substrate and phase. Proteins specifying catabolism via benzoate dihydroxylation and catechol ortho-cleavage (ben-cat pathway) were approximately an order of magnitude more abundant in benzoate- versus biphenyl-grown cells at the same growth phase. The chromosomal copy of the benzoyl-coenzyme A (CoA) (box C) pathway was also expressed during growth on biphenyl: BoxC proteins were approximately twice as abundant as Ben and Cat proteins under these conditions. By contrast, proteins of the megaplasmid copy of the benzoyl-CoA (box M) pathway were only detected in transition-phase benzoate-grown cells. Other proteins detected at increased levels in benzoate- and biphenyl-grown cells included general stress response proteins potentially induced by reactive oxygen species formed during aerobic aromatic catabolism. Finally, C1 metabolic enzymes were present in biphenyl-grown cells during transition phase. This study provides insights into the physiological roles and integration of apparently redundant catabolic pathways in large-genome bacteria and establishes a basis for investigating the PCB-degrading abilities of this strain.

Sign in / Sign up

Export Citation Format

Share Document