Cloning, Sequencing, and Expression of a Gene Encoding the Monomeric Isocitrate Dehydrogenase of the Nitrogen-fixing Bacterium,Azotobacter vinelandii

2002 ◽  
Vol 66 (3) ◽  
pp. 489-500 ◽  
Author(s):  
Takehiko SAHARA ◽  
Yasuhiro TAKADA ◽  
Yoji TAKEUCHI ◽  
Naoto YAMAOKA ◽  
Noriyuki FUKUNAGA
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Azotobacter Vinelandii ◽  
Nitrogen Fixing ◽  
Gene Encoding ◽  
Sequencing And Expression

Characterization of the iron superoxide dismutase gene of Azotobacter vinelandii: sodB may be essential for viability

2001 ◽  
Vol 47 (1) ◽  
pp. 63-71 ◽  
Author(s):  
Barbara A Qurollo ◽  
Paul E Bishop ◽  
Hosni M Hassan
Keyword(s):  
Stationary Phase ◽  
Azotobacter Vinelandii ◽  
Cosmid Library ◽  
Insertion Mutation ◽  
Upstream Sequence ◽  
Nitrogen Fixing ◽  
Coding Region ◽  
Gene Encoding ◽  
Growth And Survival ◽  
Superoxide Dismutase Gene

Azotobacter vinelandii contains two superoxide dismutases (SODs), a cytoplasmic iron-containing enzyme (FeSOD), and a periplasmic copper/zinc-containing enzyme (CuZnSOD). In this study, the FeSOD was found to be constitutive, while the activity of CuZnSOD increased as the culture entered the stationary phase. Total SOD (units/mg protein) in stationary phase cells grown under nitrogen-fixing conditions was not significantly different from those grown under non-nitrogen-fixing conditions. The gene encoding FeSOD (sodB) was isolated from an A. vinelandii cosmid library. A 1-kb fragment containing the coding region and 400 base pairs of upstream sequence was cloned and sequenced. The nucleotide sequence and the deduced amino acid sequence had a high degree of homology with other bacterial FeSODs, particularly with P. aeruginosa. Attempts to construct a sodB mutant by recombination of a sodB::kan insertion mutation into the multicopy chromosome of A. vinelandii were unsuccessful even in the presence of SOD mimics or nutritional supplements. These results suggest that FeSOD may be essential for the growth and survival of A. vinelandii, and that the periplasmic CuZnSOD cannot replace the function of FeSOD.

Isocitrate dehydrogenase from Azotobacter vinelandii. Order of substrate addition and product release

Biochemistry ◽  
1972 ◽  
Vol 11 (25) ◽  
pp. 4766-4778 ◽  
Author(s):  
Jeffrey S. Wicken ◽  
Albert E. Chung ◽  
James S. Franzen
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Azotobacter Vinelandii ◽  
Product Release ◽  
Substrate Addition

Optimal conditions for induction of competence in nitrogen-fixing Azotobacter vinelandii

1982 ◽  
Vol 28 (4) ◽  
pp. 389-397 ◽  
Author(s):  
William J. Page
Keyword(s):  
Dissolved Oxygen ◽  
Sodium Acetate ◽  
Azotobacter Vinelandii ◽  
Nitrogenase Activity ◽  
Cell Mass ◽  
Apparent Competition ◽  
Competence Development ◽  
Optimal Conditions ◽  
Nitrogen Fixing ◽  
Ph Range

Competence development in nitrogen-fixing Azotobacter vinelandii cells was optimal at pH 7.2–7.4 which necessitated additional buffering of the iron-limited nitrogen-free competence medium or the addition of a suitable organic acid salt, e.g., sodium acetate. An autolysin was active in this pH range and competent cells were more susceptible to autolysis than the general cell population. Competence development also required restricted aeration of the culture, and only those cultures that attained zero dissolved oxygen became competent. Restricted aeration served to protect the iron-limited cell nitrogenase from oxygen inactivation thus allowing the culture to reach zero dissolved oxygen. The inclusion of additional sources of reductant, e.g., malate, in buffered competence medium resulted in increased respiration and protection of nitrogenase, increased cell mass, and poly-β-hydroxybutyrate synthesis, but decreased competence. A possible explanation for the apparent competition between competence development and nitrogenase activity is discussed.

Isolation, Characterization, and Disruption of the Yeast Gene Encoding Cytosolic NADP-Specific Isocitrate Dehydrogenase

Biochemistry ◽  
1994 ◽  
Vol 33 (32) ◽  
pp. 9661-9667 ◽  
Author(s):  
Thomas M. Loftus ◽  
Linda V. Hall ◽  
Sondra L. Anderson ◽  
Lee McAlister-Henn
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Yeast Gene ◽  
Gene Encoding

scholarly journals The nitrogen-fixing symbiotic bacteriumMesorhizobium lotihas and expresses the gene encoding pyridoxine 4-oxidase involved in the degradation of vitamin B6

2004 ◽  
Vol 234 (2) ◽  
pp. 225-230 ◽  
Author(s):  
Baiqiang Yuan ◽  
Yu Yoshikane ◽  
Nana Yokochi ◽  
Kouhei Ohnishi ◽  
Toshiharu Yagi
Keyword(s):  
Vitamin B6 ◽  
Nitrogen Fixing ◽  
Gene Encoding
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