Isolation, Characterization, and Disruption of the Yeast Gene Encoding Cytosolic NADP-Specific Isocitrate Dehydrogenase

Biochemistry ◽  
1994 ◽  
Vol 33 (32) ◽  
pp. 9661-9667 ◽  
Author(s):  
Thomas M. Loftus ◽  
Linda V. Hall ◽  
Sondra L. Anderson ◽  
Lee McAlister-Henn
Keyword(s):  
Isocitrate Dehydrogenase ◽  
Yeast Gene ◽  
Gene Encoding

An essential yeast gene encoding a TTAGGG repeat-binding protein

1993 ◽  
Vol 13 (2) ◽  
pp. 1306-1314
Author(s):  
C Brigati ◽  
S Kurtz ◽  
D Balderes ◽  
G Vidali ◽  
D Shore
Keyword(s):  
Dna Binding ◽  
Binding Protein ◽  
Binding Activity ◽  
Insertion Mutation ◽  
Yeast Gene ◽  
Gene Encoding ◽  
Cloned Gene ◽  
Diploid Cells ◽  
Ttaggg Repeat

A yeast gene encoding a DNA-binding protein that recognizes the telomeric repeat sequence TTAGGG found in multicellular eukaryotes was identified by screening a lambda gt11 expression library with a radiolabeled TTAGGG multimer. This gene, which we refer to as TBF1 (TTAGGG repeat-binding factor 1), encodes a polypeptide with a predicted molecular mass of 63 kDa. The TBF1 protein, produced in vitro by transcription and translation of the cloned gene, binds to (TTAGGG)n probes and to a yeast telomeric junction sequence that contains two copies of the sequence TTAGGG separated by 5 bp. TBF1 appears to be identical to a previously described yeast TTAGGG-repeat binding activity called TBF alpha. TBF1 produced in vitro yields protein-DNA complexes with (TTAGGG)n probes that have mobilities on native polyacrylamide gels identical to those produced by partially purified TBF alpha from yeast cells. Furthermore, when extracts are prepared from a strain containing a TBF1 gene with an antigen tag, we find that the antigen copurifies with the predominant (TTAGGG)n-binding activity in the extracts. The DNA sequence of TBF1 was determined. The predicted protein sequence suggests that TBF1 may contain a nucleotide-binding domain, but no significant similarities to any other known proteins were identified, nor was an obvious DNA-binding motif apparent. Diploid cells heterozygous for a tbf1::URA3 insertion mutation are viable but upon sporulation give rise to tetrads with only two viable spores, both of which are Ura-, indicating that the TBF1 gene is essential for growth. Possible functions of TBF1 (TFB alpha) are discussed in light of these new results.

Yeast sequencing reports.APL1, a yeast gene encoding a putative permease for basic amino acids

Yeast ◽  
1994 ◽  
Vol 10 (5) ◽  
pp. 653-657 ◽  
Author(s):  
Hana Sychrova ◽  
Marie-Renee Chevallier
Keyword(s):  
Amino Acids ◽  
Yeast Gene ◽  
Gene Encoding ◽  
Basic Amino Acids

scholarly journals Isolation and analysis of the fission yeast gene encoding polymerase delta accessory protein PCNA.

1992 ◽  
Vol 11 (13) ◽  
pp. 5111-5120 ◽  
Author(s):  
N.H. Waseem ◽  
K. Labib ◽  
P. Nurse ◽  
D.P. Lane
Keyword(s):  
Fission Yeast ◽  
Accessory Protein ◽  
Yeast Gene ◽  
Gene Encoding

Chloroplast Development at Low Temperatures Requires a Homolog of DIM1, a Yeast Gene Encoding the 18S rRNA Dimethylase

1998 ◽  
Vol 10 (5) ◽  
pp. 699
Author(s):  
James G. Tokuhisa ◽  
Perumal Vijayan ◽  
Kenneth A. Feldmann ◽  
John A. Browse
Keyword(s):  
Low Temperatures ◽  
18S Rrna ◽  
Chloroplast Development ◽  
Yeast Gene ◽  
Gene Encoding

Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase

1994 ◽  
Vol 221 (2) ◽  
pp. 741-747 ◽  
Author(s):  
Michael HOFMANN ◽  
Eckhard BOLES ◽  
Friedrich K. ZIMMERMANN
Keyword(s):  
Yeast Gene ◽  
Gene Encoding

A yeast gene encoding a putative RNA helicase of the “DEAD”-box family

Yeast ◽  
1993 ◽  
Vol 9 (4) ◽  
pp. 429-432 ◽  
Author(s):  
Sabine Strahl-Bolsinger ◽  
Widmar Tanner
Keyword(s):  
Rna Helicase ◽  
Yeast Gene ◽  
Gene Encoding ◽  
Dead Box ◽  
The Dead

scholarly journals Mutations in RAD6, a yeast gene encoding a ubiquitin-conjugating enzyme, stimulate retrotransposition.

1990 ◽  
Vol 10 (3) ◽  
pp. 1017-1022 ◽  
Author(s):  
S Picologlou ◽  
N Brown ◽  
S W Liebman
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fold Increase ◽  
Yeast Gene ◽  
Gene Encoding ◽  
Repair Gene ◽  
Dna Repair Gene ◽  
Ubiquitin Conjugating Enzyme ◽  
First Time ◽  
Conjugating Enzyme

The Saccharomyces cerevisiae DNA repair gene RAD6 encodes a ubiquitin-conjugating enzyme which polyubiquitinates histones in vitro. Here we show that mutations in rad6 increase the frequency of transposition of the retrotransposon Ty into the CAN1 and URA3 loci. Using isogenic RAD6 and rad6 strains, we measured a more than 100-fold increase in the spontaneous rate of retrotransposition due to rad6, although there was no increase in the Ty message level. This is the first time that a mutation in a host gene has been shown to result in an increased rate of retrotransposition.

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