scholarly journals Identification of Zerumbone inZingiber zerumbetSmith as a Potent Inhibitor of 12-O-Tetradecanoylphorbol-13-acetate-induced Epstein-Barr Virus Activation

1999 ◽  
Vol 63 (10) ◽  
pp. 1811-1812 ◽  
Author(s):  
Akira MURAKAMI ◽  
Miyuki TAKAHASHI ◽  
Suratwadee JIWAJINDA ◽  
Koichi KOSHIMIZU ◽  
Hajime OHIGASHI
Keyword(s):  
Epstein Barr Virus ◽  
Potent Inhibitor ◽  
Barr Virus ◽  
Epstein Barr

1′-Acetoxychavicol Acetate as a Potent Inhibitor of Tumor Promoter-induced Epstein-Barr Virus Activation fromLanguas gaianga, a Traditional Thai Condiment

1993 ◽  
Vol 57 (8) ◽  
pp. 1344-1345 ◽  
Author(s):  
Akira Kondo ◽  
Hajime Ohigashi ◽  
Akira Murakami ◽  
Jiwajinda Suratwadee ◽  
Koichi Koshimizu
Keyword(s):  
Epstein Barr Virus ◽  
Potent Inhibitor ◽  
Tumor Promoter ◽  
Barr Virus ◽  
Epstein Barr

scholarly journals The Epstein-Barr Virus LF2 Protein Inhibits Viral Replication

2008 ◽  
Vol 82 (17) ◽  
pp. 8509-8519 ◽  
Author(s):  
Michael A. Calderwood ◽  
Amy M. Holthaus ◽  
Eric Johannsen
Keyword(s):  
Transcriptional Activation ◽  
Epstein Barr Virus ◽  
Reference Strain ◽  
Latent Infection ◽  
Potent Inhibitor ◽  
Lytic Replication ◽  
Future Studies ◽  
Barr Virus ◽  
Infected Cells ◽  
Epstein Barr

ABSTRACT The switch from Epstein-Barr virus (EBV) latent infection to lytic replication is governed by two transcriptional regulators, Zta and Rta. We previously reported that the EBV protein encoded by the LF2 gene binds to Rta and can inhibit Rta activity in reporter gene assays. We now report that LF2 associates with Rta in the context of EBV-infected cells induced for lytic replication. LF2 inhibition of Rta occurs in both epithelial and B cells, and this downregulation is promoter specific: LF2 decreases Rta activation of the BALF2, BMLF1, and BMRF1 promoters by 60 to 90% but does not significantly decrease Rta activation of its own promoter (Rp). LF2 decreases Rta activation by at least two mechanisms: decreased DNA binding and interference with transcriptional activation by the Rta acidic activation domain. Coexpression of LF2 also specifically induces modification of Rta by the small ubiquitin-like modifiers SUMO2 and SUMO3. We further demonstrate that LF2 overexpression blocks lytic activation in EBV-infected cells induced with Rta or Zta. Our results demonstrate that LF2, a gene deleted from the EBV reference strain B95-8, encodes a potent inhibitor of EBV replication, and they suggest that future studies of EBV replication need to account for the potential effects of LF2 on Rta activity.

scholarly journals Synthesis of Methylenecyclobutane Analogues of Nucleosides with Axial Chirality and Their Phosphoralaninates: A New Pronucleotide Effective against Epstein-Barr Virus

2002 ◽  
Vol 13 (4) ◽  
pp. 251-262 ◽  
Author(s):  
Ruifang Wang ◽  
Earl R Kern ◽  
Jiri Zemlicka
Keyword(s):  
Cell Culture ◽  
Adenosine Deaminase ◽  
Epstein Barr Virus ◽  
Potent Inhibitor ◽  
Antiviral Agents ◽  
Porcine Liver ◽  
Daudi Cell ◽  
Barr Virus ◽  
Liver Esterase ◽  
Epstein Barr

Methylenecyclobutane analogues of 2′-deoxyadenosine, 2′-deoxyguanosine and 2′- deoxycytidine, and the corresponding phosphoralaninate pronucleotides comprising adenine and guanine bases, were synthesized as potential antiviral agents. Phosphoralaninate of adenine methylenecyclobutane was a potent inhibitor of replication of Epstein-Barr virus (EBV) in Daudi cell culture. Phosphoralaninate of guanine analogue was inactive but both pronucleotides were substrates for porcine liver esterase. Adenine methylenecyclobutane analogue was deaminated by adenosine deaminase.

A Quantitative Ultrastructural Study of Peripheral Blood Lymphocytes Containing Parallel Tubular Arrays in Epstein-Barr Virus and Cytomegalo-Virus Mononucleosis

1981 ◽  
Vol 39 ◽  
pp. 454-455
Author(s):  
C. M. Payne ◽  
P. M. Tennican
Keyword(s):  
Peripheral Blood ◽  
Lymphocyte Count ◽  
Epstein Barr Virus ◽  
Absolute Lymphocyte Count ◽  
Peripheral Blood Lymphocytes ◽  
Clinical State ◽  
Barr Virus ◽  
The Absolute ◽  
Epstein Barr ◽  
Tubular Arrays

In the normal peripheral circulation there exists a sub-population of lymphocytes which is ultrastructurally distinct. This lymphocyte is identified under the electron microscope by the presence of cytoplasmic microtubular-like inclusions called parallel tubular arrays (PTA) (Figure 1), and contains Fc-receptors for cytophilic antibody. In this study, lymphocytes containing PTA (PTA-lymphocytes) were quantitated from serial peripheral blood specimens obtained from two patients with Epstein -Barr Virus mononucleosis and two patients with cytomegalovirus mononucleosis. This data was then correlated with the clinical state of the patient.It was determined that both the percentage and absolute number of PTA- lymphocytes was highest during the acute phase of the illness. In follow-up specimens, three of the four patients' absolute lymphocyte count fell to within normal limits before the absolute PTA-lymphocyte count.In one patient who was followed for almost a year, the absolute PTA- lymphocyte count was consistently elevated (Figure 2). The estimation of absolute PTA-lymphocyte counts was determined to be valid after a morphometric analysis of the cellular areas occupied by PTA during the acute and convalescent phases of the disease revealed no statistical differences.

Studies of Epstein-Barr Virus Antigens Using Immunoperoxidase and Immunofluorescence Techniques

1975 ◽  
Vol 33 ◽  
pp. 342-343
Author(s):  
R. Stephens ◽  
K. Traul ◽  
D. Woolf ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
If Technique ◽  
Infected Cells ◽  
Virus Antigens ◽  
Epstein Barr ◽  
Virus Capsid Antigen ◽  
Antigen Reactivity

A number of antigens have been found associated with persistent EBV infections of lymphoblastoid cells. Identification and localization of these antigens were principally by immunofluorescence (IF) techniques using sera from patients with nasopharyngeal carcinoma (NPC), Burkitt lymphoma (BL), and infectious mononucleosis (IM). Our study was mainly with three of the EBV related antigens, a) virus capsid antigen (VCA), b) membrane antigen (MA), and c) early antigens (EA) using immunoperoxidase (IP) techniques with electron microscopy (EM) to elucidate the sites of reactivity with EBV and EBV infected cells.Prior to labeling with horseradish peroxidase (HRP), sera from NPC, IM, and BL cases were characterized for various reactivities by the indirect IF technique. Modifications of the direct IP procedure described by Shabo and the indirect IP procedure of Leduc were made to enhance penetration of the cells and preservation of antigen reactivity.

Quantitative analysis of circulating cell-free Epstein-Barr virus (EBV) DNA levels in patients with EBV-associated lymphoid malignancies

2000 ◽  
Vol 111 (1) ◽  
pp. 239-246 ◽  
Author(s):  
Kenny I. K. Lei ◽  
Lisa Y.S. Chan ◽  
Wing Y. Chan ◽  
Philip J. Johnson ◽  
Y. M. Dennis Lo
Keyword(s):  
Quantitative Analysis ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Lymphoid Malignancies ◽  
Ebv Dna ◽  
Epstein Barr

Chronic bullous disease of childhood following Epstein-Barr virus seroconversion: a case report

1996 ◽  
Vol 21 (2) ◽  
pp. 123-126
Author(s):  
U. BALDARI ◽  
A. ASCARI RACCAGNI ◽  
B. CELLI ◽  
M. GIOVANNA RIGHINI
Keyword(s):  
Case Report ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
Bullous Disease ◽  
Epstein Barr
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