Expression and Purification of Cytokine Receptor Homology Domain of Human Granulocyte-Colony Stimulating Factor Receptor inEscherichia coli

1998 ◽  
Vol 62 (9) ◽  
pp. 1809-1811 ◽  
Author(s):  
Ryoichi TANAKA ◽  
Hiroko TOKUNAGA ◽  
Shinichi HARA ◽  
Tsutomu ARAKAWA ◽  
Masao TOKUNAGA
Keyword(s):  
Cytokine Receptor ◽  
Inescherichia Coli ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Expression And Purification ◽  
Stimulating Factor

scholarly journals Effects of granulocyte colony-stimulating factor on plasma cytokine and cytokine receptor levels and on the in vivo host response to endotoxin in healthy men

Blood ◽  
1996 ◽  
Vol 87 (3) ◽  
pp. 900-905 ◽  
Author(s):  
T Pollmacher ◽  
C Korth ◽  
J Mullington ◽  
W Schreiber ◽  
J Sauer ◽  
...  
Keyword(s):  
Plasma Levels ◽  
Host Response ◽  
Cytokine Receptor ◽  
Tnf Alpha ◽  
Control Group ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Healthy Humans ◽  
Stimulating Factor

We investigated the effects of granulocyte colony-stimulating factor (G- CSF) on cytokine and cytokine receptor plasma levels and on the in vivo host response to Salmonella abortus equi endotoxin in healthy males. Twenty volunteers received 0.8 ng/kg endotoxin and saline intravenously 1 week apart in randomized order. Twelve hours before both experiments, 10 of these subjects were pretreated with 300 micrograms G-CSF subcutaneously. G-CSF itself increased granulocyte and monocyte counts and the plasma levels of tumor necrosis factor-alpha (TNF-alpha), soluble TNF receptors (sTNF-R) p55, and p75 and interleukin-1 receptor antagonist (IL-1ra). G-CSF did not influence plasma IL-1 beta and IL-6 levels. In the G-CSF-pretreated subjects endotoxin-induced surges in rectal temperature and in the plasma levels of TNF-alpha plasma levels were about 50% increased, and surges in the plasma levels of both sTNF- Rs and IL-1ra were about twice as high as in the control group. Endotoxin-induced increases in IL-6, cortisol, and heart rate were not modified by G-CSF pretreatment. Endotoxin administration induced a transient 50% reduction in leukocyte counts in the G-CSF-pretreated subjects that was not seen in the control group. We conclude that a single stand dose of G-CSF increases the plasma levels of cytokines and cytokine receptors and considerably modifies the host response of healthy humans to a low dose of endotoxin.

Expression and purification of canine granulocyte colony-stimulating factor (cG-CSF)

2009 ◽  
Vol 130 (3-4) ◽  
pp. 221-225 ◽  
Author(s):  
Akira Yamamoto ◽  
Akira Iwata ◽  
Toshiki Saito ◽  
Fumiko Watanabe ◽  
Susumu Ueda
Keyword(s):  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Expression And Purification ◽  
Stimulating Factor
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