Expression of cDNA for recombinant human granulocyte colony-stimulating factor inEscherichia coli and characterization of the protein

1998 ◽  
Vol 10 (4) ◽  
pp. 256-259 ◽  
Author(s):  
Shu Zhang ◽  
Qinong Ye
Keyword(s):  
Inescherichia Coli ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Stimulating Factor

scholarly journals Characterization of recombinant-derived granulocyte-colony stimulating factor (G-CSF)

1988 ◽  
Vol 256 (1) ◽  
pp. 213-218 ◽  
Author(s):  
P Wingfield ◽  
R Benedict ◽  
G Turcatti ◽  
B Allet ◽  
J J Mermod ◽  
...  
Keyword(s):  
Coli Strain ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Wild Type ◽  
Disulphide Bonds ◽  
Disulphide Bond Formation ◽  
Stimulating Factor ◽  
Factor G

Human granulocyte colony-stimulating factor (G-CSF), and a mutant having a Ser for Cys substitution at residue 18 were produced in Escherichia coli strain W3110. About 60 mg of pure protein was obtained from 50 g of wet cells with a recovery of about 20%. The proteins were characterized physically and chemically, including determination of disulphide bonds, which were found to exist between residues 37-43 and 65-75. Cys-18 is not involved in disulphide bond formation and was substituted by Ser with no effects on gross protein conformation or biological activity. Both the wild-type and the mutant recombinant-derived proteins, although not glycosylated, possess colony-stimulating activities. In a bioassay using the murine myelomonocytic leukaemic cell line WEH1 3B D+, activities were obtained which were similar to those of natural G-CSF and of a glycosylated recombinant-derived human G-CSF produced in monkey cells.

Construction, Purification, and Characterization of a Homodimeric Granulocyte Colony-Stimulating Factor

2017 ◽  
Vol 59 (9-10) ◽  
pp. 374-384 ◽  
Author(s):  
Gitana Mickiene ◽  
Indre Dalgediene ◽  
Zilvinas Dapkunas ◽  
Gintautas Zvirblis ◽  
Henrikas Pesliakas ◽  
...  
Keyword(s):  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Purification And Characterization ◽  
Stimulating Factor

Characterization of the receptor binding determinants of granulocyte colony stimulating factor

1997 ◽  
Vol 6 (8) ◽  
pp. 1787-1787
Author(s):  
Dennis C. Young ◽  
Qi-Lin Cheng ◽  
Jinzhao Hou ◽  
David J. Matthews ◽  
Hangjun Zhan
Keyword(s):  
Receptor Binding ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Binding Determinants ◽  
Stimulating Factor
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