scholarly journals Effects of Batatasin III and Its Analogs on Gibberellic Acid-Dependent α-Amylase Induction in Embryoless Barley Seeds and on Cress Growth

1998 ◽  
Vol 62 (8) ◽  
pp. 1619-1620 ◽  
Author(s):  
Hiroshi ASAHINA ◽  
Hiromichi YOSHIKAWA ◽  
Yoshihiro SHUTO
1982 ◽  
Vol 9 (6) ◽  
pp. 623 ◽  
Author(s):  
RA Gibson ◽  
LG Paleg

We have examined a number of parameters relating to the gibberellic acid (GA3) requirement for the induction process which leads to the formation of �-amylase in wheat aleurone tissue. (1) Over a wide range of hormone concentrations, aleurone tissue was found to produce less �-amylase after hormone withdrawal by washing at a time soon after GA*3 application. (2) The approximate length of this washing-sensitive period was found to vary inversely to the applied GA*3 concentration. (3) Following this early period, a time was found when the response could not be lowered by this same washing procedure which had earlier lowered the response. (4) Separate experiments determined that these results could not be accounted for by variation in the lag/latent period, since the lag period was about the same for a wide range of GA*3 concentrations. To help explain these results we have suggested the term 'site saturation time' which is separate from but intimately involved with the lagllatent period. This term is defined as the time required for the saturation and activation of all sites which ultimately lead to the production of �-amylase. It is suggested that site saturation time must be taken into account when the early events of �-amylase induction are examined.


1974 ◽  
Vol 1 (3) ◽  
pp. 343 ◽  
Author(s):  
JV Jacobsen ◽  
JA Zwar

RNA containing polyadenylic acid sequences [poly(A)-RNA] was detected in and isolated from barley aleurone layers. Gibberellic acid (GA3) stimulated the synthesis of this RNA by about 70% over a 16-h period during which amylase induction occurred. The poly(A)-RNA was polydisperse on polyacrylamide gels and the effect of GA3 was to enhance synthesis of poly(A)-RNA species with a wide range of molecular weights. GA3 had no major effects on the synthesis of rRNA, 5 S RNA and tRNA species, and since it also had no effect on levels of total RNA in aleurone, it probably had no effect on RNA breakdown. Furthermore, rRNA synthesis could be essentially stopped with 5-fluorouracil with no effect on amylase synthesis, indicating that synthesis of new ribosomes was unnecessary for normal amylase induction. These results indicate that for amylase synthesis to occur in response to GA3, regulation of synthesis of mRNA but not that of the major species would appear to be necessary.


1994 ◽  
Vol 62 (4) ◽  
pp. 867-875 ◽  
Author(s):  
Hiroyuki Itamura ◽  
Tadaaki Fukushima ◽  
Toshio Kitamura ◽  
Hisashi Harada ◽  
Satoshi Taira ◽  
...  

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