RNase E Is Required for Induction of the Glutamate-Dependent Acid Resistance System inEscherichia coli

2007 ◽  
Vol 71 (1) ◽  
pp. 158-164 ◽  
Author(s):  
Ayako TAKADA ◽  
Genryou UMITSUKI ◽  
Kazuo NAGAI ◽  
Masaaki WACHI
Keyword(s):  
Acid Resistance ◽  
Inescherichia Coli ◽  
Rnase E ◽  
Resistance System

scholarly journals Antagonistic Role of H-NS and GadX in the Regulation of the Glutamate Decarboxylase-dependent Acid Resistance System inEscherichia coli

2005 ◽  
Vol 280 (22) ◽  
pp. 21498-21505 ◽  
Author(s):  
Mara Giangrossi ◽  
Stefano Zattoni ◽  
Angela Tramonti ◽  
Daniela De Biase ◽  
Maurizio Falconi
Keyword(s):  
Glutamate Decarboxylase ◽  
Acid Resistance ◽  
Inescherichia Coli ◽  
Resistance System

scholarly journals SspA is required for acid resistance in stationary phase by downregulation of H-NS inEscherichia coli

2005 ◽  
Vol 56 (3) ◽  
pp. 719-734 ◽  
Author(s):  
Anne-Marie Hansen ◽  
Yu Qiu ◽  
Norman Yeh ◽  
Frederick R. Blattner ◽  
Tim Durfee ◽  
...  
Keyword(s):  
Stationary Phase ◽  
Acid Resistance ◽  
Inescherichia Coli

scholarly journals Characterization of mutations in the PAS domain of the EvgS sensor kinase selected by laboratory evolution for acid resistance inEscherichia coli

2014 ◽  
Vol 93 (5) ◽  
pp. 911-927 ◽  
Author(s):  
Matthew D. Johnson ◽  
James Bell ◽  
Kim Clarke ◽  
Rachel Chandler ◽  
Prachi Pathak ◽  
...  
Keyword(s):  
Acid Resistance ◽  
Sensor Kinase ◽  
Pas Domain ◽  
Inescherichia Coli ◽  
Laboratory Evolution

scholarly journals Transcriptional Expression of Escherichia coli Glutamate-Dependent Acid Resistance Genes gadA and gadBC in an hns rpoS Mutant

2003 ◽  
Vol 185 (15) ◽  
pp. 4644-4647 ◽  
Author(s):  
Scott R. Waterman ◽  
P. L. C. Small
Keyword(s):  
Escherichia Coli ◽  
Acid Resistance ◽  
Sigma Factors ◽  
Transcriptional Expression ◽  
E Coli ◽  
Ph Values ◽  
Rpos Mutant ◽  
Resistance System ◽  
Acidic Environments ◽  
K 12

ABSTRACT Resistance to being killed by acidic environments with pH values lower than 3 is an important feature of both pathogenic and nonpathogenic Escherichia coli. The most potent E. coli acid resistance system utilizes two isoforms of glutamate decarboxylase encoded by gadA and gadB and a putative glutamate:γ-aminobutyric acid antiporter encoded by gadC. The gad system is controlled by two repressors (H-NS and CRP), one activator (GadX), one repressor-activator (GadW), and two sigma factors (σS and σ70). In contrast to results of previous reports, we demonstrate that gad transcription can be detected in an hns rpoS mutant strain of E. coli K-12, indicating that gad promoters can be initiated by σ70 in the absence of H-NS.

scholarly journals Biochemical and spectroscopic properties ofBrucella microtiglutamate decarboxylase, a key component of the glutamate-dependent acid resistance system

FEBS Open Bio ◽  
2015 ◽  
Vol 5 (1) ◽  
pp. 209-218 ◽  
Author(s):  
Gaia Grassini ◽  
Eugenia Pennacchietti ◽  
Francesca Cappadocio ◽  
Alessandra Occhialini ◽  
Daniela De Biase
Keyword(s):  
Acid Resistance ◽  
Spectroscopic Properties ◽  
Resistance System

scholarly journals Overexpression of an antisense RNA, ArrS, increases the acid resistance of Escherichia coli

Microbiology ◽  
2014 ◽  
Vol 160 (5) ◽  
pp. 954-961 ◽  
Author(s):  
Toshiko Aiso ◽  
Shigeru Kamiya ◽  
Hideo Yonezawa ◽  
Shinobu Gamou
Keyword(s):  
Escherichia Coli ◽  
Antisense Rna ◽  
Acid Resistance ◽  
Stationary Growth Phase ◽  
Rnase Iii ◽  
Stationary Growth ◽  
E Coli ◽  
Resistance System ◽  
Acidic Conditions

The antisense RNA ArrS is complementary to a sequence in the 5′ untranslated region of the gadE T3 mRNA, the largest transcript of gadE, which encodes a transcriptional activator of the glutamate-dependent acid resistance system in Escherichia coli. Expression of arrS is strongly induced during the stationary growth phase, particularly under acidic conditions, and transcription is dependent on σS and GadE. The aim of the present study was to clarify the role of ArrS in controlling gadE expression by overexpressing arrS in E. coli. The results showed a marked increase in the survival of arrS-overexpressing cells at 2 h after a shift to pH 2.5. This was accompanied by increased expression of gadA, gadBC and gadE. The level of gadE T3 mRNA decreased markedly in response to arrS overexpression, and was accompanied by a marked increase in gadE mRNA T2. T2 mRNA had a monophosphorylated 5′ terminus, which is usually found in cleaved mRNAs, and no T2 mRNA was observed in an RNase III-deficient cell strain. In addition, T2 mRNA was not generated by a P3-deleted gadE–luc translational fusion. These results suggest strongly that T2 mRNA is generated via the processing of T3 mRNA. Moreover, the T2 mRNA, which was abundant in arrS-overexpressing cells, was more stable than T3 mRNA in non-overexpressing cells. These results suggest that overexpression of ArrS positively regulates gadE expression in a post-transcriptional manner.

scholarly journals Activators of the Glutamate-Dependent Acid Resistance System Alleviate Deleterious Effects of YidC Depletion in Escherichia coli

2011 ◽  
Vol 193 (6) ◽  
pp. 1308-1316 ◽  
Author(s):  
Z. Yu ◽  
M. Bekker ◽  
A. Tramonti ◽  
G. M. Cook ◽  
P. van Ulsen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Acid Resistance ◽  
Resistance System
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