scholarly journals Antagonistic Role of H-NS and GadX in the Regulation of the Glutamate Decarboxylase-dependent Acid Resistance System inEscherichia coli

2005 ◽  
Vol 280 (22) ◽  
pp. 21498-21505 ◽  
Author(s):  
Mara Giangrossi ◽  
Stefano Zattoni ◽  
Angela Tramonti ◽  
Daniela De Biase ◽  
Maurizio Falconi
Keyword(s):  
Glutamate Decarboxylase ◽  
Acid Resistance ◽  
Inescherichia Coli ◽  
Resistance System

Role of the Glutamate Decarboxylase Acid Resistance System in the Survival of Listeria monocytogenes LO28 in Low pH Foods

2001 ◽  
Vol 64 (9) ◽  
pp. 1362-1368 ◽  
Author(s):  
PAUL D. COTTER ◽  
KAREN O'REILLY ◽  
COLIN HILL
Keyword(s):  
Listeria Monocytogenes ◽  
Glutamate Decarboxylase ◽  
Monosodium Glutamate ◽  
Foodborne Pathogen ◽  
Skim Milk ◽  
Acid Resistance ◽  
Low Ph ◽  
Enhanced Sensitivity ◽  
Resistance System

The glutamate decarboxylase (GAD) acid resistance system of the foodborne pathogen Listeria monocytogenes plays a major role in its survival at low pH. It was found that survival of the wild-type strain, LO28, in acidified reconstituted skim milk, diluted to reduce free glutamate levels, improves in response to supplementation with monosodium glutamate. A mutant, in which the two listerial GAD homologs have been deleted (and in which there is no discernible GAD activity), did not respond to glutamate supplementation and displayed greatly enhanced sensitivity in a number of low pH foods, even when levels of free glutamate were as low as 0.22 mM. We thus show that the GAD system plays a major role in the survival of L. monocytogenes in acidic foods even when levels of free glutamate are low.

RNase E Is Required for Induction of the Glutamate-Dependent Acid Resistance System inEscherichia coli

2007 ◽  
Vol 71 (1) ◽  
pp. 158-164 ◽  
Author(s):  
Ayako TAKADA ◽  
Genryou UMITSUKI ◽  
Kazuo NAGAI ◽  
Masaaki WACHI
Keyword(s):  
Acid Resistance ◽  
Inescherichia Coli ◽  
Rnase E ◽  
Resistance System

scholarly journals Overexpression of an antisense RNA, ArrS, increases the acid resistance of Escherichia coli

Microbiology ◽  
2014 ◽  
Vol 160 (5) ◽  
pp. 954-961 ◽  
Author(s):  
Toshiko Aiso ◽  
Shigeru Kamiya ◽  
Hideo Yonezawa ◽  
Shinobu Gamou
Keyword(s):  
Escherichia Coli ◽  
Antisense Rna ◽  
Acid Resistance ◽  
Stationary Growth Phase ◽  
Rnase Iii ◽  
Stationary Growth ◽  
E Coli ◽  
Resistance System ◽  
Acidic Conditions

The antisense RNA ArrS is complementary to a sequence in the 5′ untranslated region of the gadE T3 mRNA, the largest transcript of gadE, which encodes a transcriptional activator of the glutamate-dependent acid resistance system in Escherichia coli. Expression of arrS is strongly induced during the stationary growth phase, particularly under acidic conditions, and transcription is dependent on σS and GadE. The aim of the present study was to clarify the role of ArrS in controlling gadE expression by overexpressing arrS in E. coli. The results showed a marked increase in the survival of arrS-overexpressing cells at 2 h after a shift to pH 2.5. This was accompanied by increased expression of gadA, gadBC and gadE. The level of gadE T3 mRNA decreased markedly in response to arrS overexpression, and was accompanied by a marked increase in gadE mRNA T2. T2 mRNA had a monophosphorylated 5′ terminus, which is usually found in cleaved mRNAs, and no T2 mRNA was observed in an RNase III-deficient cell strain. In addition, T2 mRNA was not generated by a P3-deleted gadE–luc translational fusion. These results suggest strongly that T2 mRNA is generated via the processing of T3 mRNA. Moreover, the T2 mRNA, which was abundant in arrS-overexpressing cells, was more stable than T3 mRNA in non-overexpressing cells. These results suggest that overexpression of ArrS positively regulates gadE expression in a post-transcriptional manner.

scholarly journals The glutamate-dependent acid resistance system in Escherichia coli: essential and dual role of the His–Asp phosphorelay RcsCDB/AF

Microbiology ◽  
2007 ◽  
Vol 153 (1) ◽  
pp. 238-246 ◽  
Author(s):  
Marie-Pierre Castanié-Cornet ◽  
Hélène Treffandier ◽  
Anne Francez-Charlot ◽  
Claude Gutierrez ◽  
Kaymeuang Cam
Keyword(s):  
Escherichia Coli ◽  
Acid Resistance ◽  
Dual Role ◽  
Resistance System

scholarly journals Important Role of the Amino Acid Attached to tRNA in Formylation and in Initiation of Protein Synthesis inEscherichia coli

1996 ◽  
Vol 271 (2) ◽  
pp. 1022-1028 ◽  
Author(s):  
Shihong Li ◽  
N. Vinay Kumar ◽  
Umesh Varshney ◽  
Uttam L. RajBhandary
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Inescherichia Coli

scholarly journals The role of the omega subunit of RNA polymerase in expression of therelAgene inEscherichia coli

2007 ◽  
Vol 267 (1) ◽  
pp. 51-55 ◽  
Author(s):  
Dipankar Chatterji ◽  
Yoshito Ogawa ◽  
Tomohiro Shimada ◽  
Akira Ishihama
Keyword(s):  
Rna Polymerase ◽  
Inescherichia Coli

scholarly journals Protein aggregation inEscherichia coli: role of proteases

2002 ◽  
Vol 207 (1) ◽  
pp. 9-12 ◽  
Author(s):  
Ran Rosen ◽  
Dvora Biran ◽  
Eyal Gur ◽  
Dörte Becher ◽  
Michael Hecker ◽  
...  
Keyword(s):  
Protein Aggregation ◽  
Inescherichia Coli
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