Mixed-Species Biofilm Formation by Direct Cell-Cell Contact between Brewing Yeasts and Lactic Acid Bacteria

2010 ◽  
Vol 74 (11) ◽  
pp. 2316-2319 ◽  
Author(s):  
Soichi FURUKAWA ◽  
Kanako YOSHIDA ◽  
Hirokazu OGIHARA ◽  
Makari YAMASAKI ◽  
Yasushi MORINAGA
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biofilm Formation ◽  
Cell Contact ◽  
Mixed Species ◽  
Direct Cell ◽  
Brewing Yeasts ◽  
Cell Cell

scholarly journals Mixed-Species Biofilm Formation by Lactic Acid Bacteria and Rice Wine Yeasts

2007 ◽  
Vol 73 (14) ◽  
pp. 4673-4676 ◽  
Author(s):  
Taketo Kawarai ◽  
Soichi Furukawa ◽  
Hirokazu Ogihara ◽  
Makari Yamasaki
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biofilm Formation ◽  
Lactobacillus Casei ◽  
Yeast Cells ◽  
Rice Wine ◽  
Wine Yeasts ◽  
Mixed Species ◽  
Distinct Morphology

ABSTRACT We found that species combinations such as Lactobacillus casei subsp. rhamnosus IFO3831 and Saccharomyces cerevisiae Kyokai-10 can form a mixed-species biofilm in coculture. Moreover, the Kyokai-10 yeast strain can form a biofilm in monoculture in the presence of conditioned medium (CM) from L. casei IFO3831. The active substance(s) in bacterial CM is heat sensitive and has a molecular mass of between 3 and 5 kDa. In biofilms from cocultures or CM monocultures, yeast cells had a distinct morphology, with many hill-like protrusions on the cell surface.

scholarly journals Mixed-Species Biofilm Formation by Lactic Acid Bacteria and Rice Wine Yeasts

2008 ◽  
Vol 74 (24) ◽  
pp. 7835-7835
Author(s):  
Taketo Kawarai ◽  
Soichi Furukawa ◽  
Hirokazu Ogihara ◽  
Makari Yamasaki
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Biofilm Formation ◽  
Rice Wine ◽  
Wine Yeasts ◽  
Mixed Species

Abstract 15392: Inflammation and Ischemic Heart Failure: Monocyte-Mediated Cardiac Fibroblast Activation and Matrix Remodeling Through a Direct Cell-Cell Contact Mechanism

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Holly E Mewhort ◽  
Brodie D Lipon ◽  
Daniyil A Svystonyuk ◽  
David G Guzzardi ◽  
Paul W Fedak
Keyword(s):  
Peripheral Blood ◽  
Cardiac Fibroblast ◽  
Cell Contact ◽  
Β1 Integrin ◽  
Blood Monocytes ◽  
Peripheral Blood Monocytes ◽  
Fibroblast Activation ◽  
Direct Cell ◽  
Tgf Β1 ◽  
Cell Cell

BACKGROUND: Following myocardial infarction (MI), activated cardiac myofibroblasts facilitate extracellular matrix (ECM) remodeling to prevent mechanical complications. However, prolonged myofibroblast activity leads to dysregulation of the ECM, maladaptive remodeling, fibrosis and heart failure (HF). Chronic inflammation is believed to drive persistent myofibroblast activity, however, the mechanisms are unclear. In this study, we explored the effects of peripheral blood monocytes on human cardiac fibroblast activation in a 3D ECM microenvironment. METHODS/RESULTS: Human cardiac fibroblasts isolated from surgical human heart biopsies were seeded into 3D collagen matrices. Peripheral blood monocytes isolated from healthy human donors were co-cultured with fibroblasts. Monocytes increased fibroblast activation measured by collagen ECM contraction (17.9±11.1% increase; p<0.01) and resulted in local ECM remodeling observed by confocal microscopy. Under co-culture conditions that prevent cell-cell contact but allow interaction via paracrine factors, monocytes had minimal effects on fibroblast activation (6.4±7.0 vs.17.9±11.1% increase, respectively; p<0.01). Multiplex analysis of the co-culture media revealed an increase in the paracrine factors Transforming Growth Factor-beta 1 (TGF-β1) and Matrix Metalloproteinase 9 when monocytes and fibroblasts were cultured under cell-cell contact conditions (162.2±11.7pg/mL and 17.5±0.5ng/mL, respectively, vs. 21.8±5.7pg/mL and 4.9 ±0.4ng/mL; p<0.001). TGF-β1 blockade abolished monocyte induced cardiac fibroblast activation, as did β1-integrin. These data suggest direct cell-cell interaction between monocytes and cardiac fibroblasts through β1-integrin results in TGF-β1 release facilitating fibroblast activation and matrix remodeling. CONCLUSION: For the first time, we demonstrate that peripheral blood monocytes stimulate human cardiac fibroblast activation through a mechanism involving TGF-β1 release as a consequence of direct cell-cell interaction through β1-integrin. These data implicate inflammation as a driver of cardiac fibrosis post-MI, highlighting potential novel therapeutic targets for the treatment of ischemic HF.

Suppression of bacteriocin production in mixed-species cultures of lactic acid bacteria

Food Control ◽  
2013 ◽  
Vol 30 (2) ◽  
pp. 474-479 ◽  
Author(s):  
Jesús Domínguez-Manzano ◽  
Rufino Jiménez-Díaz
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Bacteriocin Production ◽  
Mixed Species

Effect of mixed species alcoholic fermentation on growth and malolactic activity of lactic acid bacteria

2019 ◽  
Vol 103 (18) ◽  
pp. 7687-7702 ◽  
Author(s):  
Vasileios Englezos ◽  
David Castrillo Cachón ◽  
Kalliopi Rantsiou ◽  
Pilar Blanco ◽  
Maurizio Petrozziello ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Lactic Acid Bacteria ◽  
Alcoholic Fermentation ◽  
Mixed Species

The Importance of Inter-Species Cell-Cell Co-Aggregation betweenLactobacillus plantarumML11-11 andSaccharomyces cerevisiaeBY4741 in Mixed-Species Biofilm Formation

2011 ◽  
Vol 75 (8) ◽  
pp. 1430-1434 ◽  
Author(s):  
Soichi FURUKAWA ◽  
Natsumi NOJIMA ◽  
Kanako YOSHIDA ◽  
Satoru HIRAYAMA ◽  
Hirokazu OGIHARA ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Mixed Species ◽  
Cell Cell

scholarly journals Mutual Enhancement of Differentiation of Osteoblasts and Osteocytes Occurs Through Direct Cell-Cell Contact

2014 ◽  
pp. n/a-n/a ◽  
Author(s):  
Koji Fujita ◽  
Qian Xing ◽  
Sundeep Khosla ◽  
David G. Monroe
Keyword(s):  
Cell Contact ◽  
Direct Cell ◽  
Cell Cell
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