scholarly journals Development of IP and SCAR markers linked to the yellow seed color gene in Brassica juncea L.

2016 ◽  
Vol 66 (2) ◽  
pp. 175-180 ◽  
Author(s):  
Zhen Huang ◽  
Lu Liu ◽  
Hong Lu ◽  
Lina Lang ◽  
Na Zhao ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Seed Color ◽  
Scar Markers ◽  
Yellow Seed ◽  
Color Gene

scholarly journals Mapping and identification of yellow seed coat color genes in Brassica juncea L.

2020 ◽  
Author(s):  
Zhen Huang ◽  
Yang Wang ◽  
Hong Lu ◽  
Xiang Liu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Seed Coat Color ◽  
Flavonoid Pathway ◽  
Yellow Seed ◽  
Color Formation ◽  
Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

Inheritance of seed color and molecular markers linked to the seed color gene in Brassica juncea

2009 ◽  
Vol 25 (1) ◽  
pp. 57-65 ◽  
Author(s):  
Aixia Xu ◽  
Zhen Huang ◽  
Chaozhi Ma ◽  
Enshi Xiao ◽  
Guangwen Tian ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Brassica Juncea ◽  
Seed Color ◽  
Color Gene

INHERITANCE OF SEED COAT COLOR IN BRASSICA JUNCEA

1979 ◽  
Vol 59 (3) ◽  
pp. 635-637 ◽  
Author(s):  
C. L. VERA ◽  
D. L. WOODS ◽  
R. K. DOWNEY
Keyword(s):  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Dominant Allele ◽  
Seed Coat Color ◽  
Yellow Seed ◽  
Brown Color

The genetics of seed coat color inheritance in Brassica juncea (L.) Coss. were studied. It was concluded that this character is controlled by two duplicate pairs of genes (R1, R2) for brown color, either of which can produce brown seed color when a single dominant allele is present. Yellow seed results when all alleles at both loci are recessive.

Transmission of an alien telocentric addition chromosome in common wheat that confers blue seed color

Genome ◽  
1989 ◽  
Vol 32 (1) ◽  
pp. 30-34 ◽  
Author(s):  
E. D. P. Whelan
Keyword(s):  
Triticum Aestivum ◽  
Common Wheat ◽  
Triticum Aestivum L ◽  
Seed Color ◽  
Chromosome Behavior ◽  
Phenotypic Markers ◽  
Telocentric Chromosome ◽  
Cytogenetic Studies ◽  
Meiotic Analyses ◽  
Color Gene

Phenotypic markers of chromosomes are useful for determining chromosome behavior in cytogenetic studies. Transmission of an alien, telocentric addition from Agropyron tricophorum (Link) Richt. that confers purple aleurone pigment and blue seed color was evaluated in common wheat (Triticum aestivum L.). Twenty-five of 2570 seeds from bagged heads of eight ditelocentric-addition sister plants were almost white rather than blue. Seven of these 25 seeds and 4 of 336 blue seeds segregated 41.4%:58.6% blue:white. Meiotic analyses showed that all plants grown from these 11 seeds were spontaneously produced monotelocentric additions. Transmission of the alien telocentric chromosome through the egg and the pollen was estimated to be 19.5 and 14.3%, respectively, based on BC1F1 seed color. About 28% of F2 and F3 seeds were blue; of these 7.4% were ditelocentric additions. The frequency of blue seed in F2 progeny from spontaneous monotelocentric additions (41.4%) was significantly greater than that of monotelocentric additions from controlled crosses (28%). Penetrance of the blue seed color gene(s) associated with the alien telocentric chromosome was good. Misclassification of seed color for 1595 BC1F1 seeds was less than 3% based on BC1F2 progeny.Key words: Agropyron tricophorum.

Inheritance of hull pubescence and seed color in annual canarygrass

2003 ◽  
Vol 83 (3) ◽  
pp. 471-474 ◽  
Author(s):  
M. A. Matus-Cádiz ◽  
P. Hucl ◽  
A. Vandenberg
Keyword(s):  
Key Words ◽  
Growth Stage ◽  
Seed Color ◽  
Breeding Line ◽  
Food Crop ◽  
Yellow Seed ◽  
Segregation Ratios ◽  
Recessive Genes ◽  
Hybrid Cross ◽  
Phalaris Canariensis

The availability of glabrous-hulled annual canarygrass (Phalaris canariensis L.) cultivars with yellow seed color may pave the way for developing this species into a food crop. The objective of this research was to study the inheritance of hull pubescence and seed color in annual canarygrass. A gametocide was applied to plants at Zadoks Growth Stage 42 to induce male sterility. CDC Maria, a glabrous-hulled and brown-seeded cultivar, was crossed with six pubescent-hulled, brownseeded annual canarygrass accessions and with CY193, a pubescent-hulled and yellow-seeded breeding line. In mono-hybrid crosses, segregation ratios of F2 populations were not significantly different from the phenotypic ratios of 3 pubescent-hulled: 1 glabrous-hulled for hull pubescence and 3 brown seeded: 1 yellow seeded for seed color. In the di-hybrid cross, a phenotypic ratio of 9 pubescent-hulled/brown seeded: 3 pubescent-hulled/yellow seeded: 3 glabrous-hulled/brown seeded: 1 glabrous-hulled/yellow seeded was observed. Glabrous-hulled and yellow seeded traits are each controlled by single recessive genes that segregate independently in annual canarygrass. Key words: Phalaris canariensis, canaryseed, inheritance, hull pubescence, seed color

Independent assortment of seed color and hairy leaf genes in Brassica rapa L.

2014 ◽  
Vol 94 (4) ◽  
pp. 615-620 ◽  
Author(s):  
Mukhlesur Rahman
Keyword(s):  
Brassica Rapa ◽  
Genetic Study ◽  
Seed Color ◽  
Yellow Seed ◽  
Independent Assortment ◽  
Backcross Populations ◽  
Joint Segregation ◽  
Brown Color

Rahman, M. 2014. Independent assortment of seed color and hairy leaf genes in Brassica rapa L. Can. J. Plant Sci. 94: 615–620. A genetic study of seed color and hairy leaf in Brassica rapa was conducted in progeny originating from the brown-seeded, hairy leaf B. rapa subsp. chinensis line and the Bangladeshi B. rapa var. trilocularis line. A joint segregation of both traits was also examined in the F2 and backcross populations. Seed color segregated into brown, yellow–brown, and yellow, which suggests that digenic control of brown or yellow–brown color was dominant over yellow seed color. Hairy leaves were found to be under monogenic control, and hairy leaf was dominant over non-hairy leaf. The data show that genes controlling seed color and hairy leaf are inherited independently.

Comparison of linseed (Linum usitatissimum L.) genotypes with respect to the content of polyunsaturated fatty acids

2012 ◽  
Vol 66 (10) ◽  
Author(s):  
Marie Bjelková ◽  
Janka Nôžková ◽  
Katarína Fatrcová-Šramková ◽  
Eva Tejklová
Keyword(s):  
Fatty Acids ◽  
Linoleic Acid ◽  
Linear Dependence ◽  
Linum Usitatissimum ◽  
Oil Content ◽  
Seed Color ◽  
Alpha Linolenic Acid ◽  
Yellow Seed ◽  
Linum Usitatissimum L ◽  
Total Fat

AbstractThe aim of our work was to characterize linseed (Linum usitatissimum L.) genotypes divided into groups with high and low content of alpha-linolenic acid (ALA). Out of 32 linseed genotypes, 68.75 % represented high alpha-linolenic genotypes and 31.25 % were genotypes with low ALA content. Proportional representation of fatty acids was realized according to the norm (Czech Office for Standards, Metrology and Testing, 1994). Oil content was analyzed according to the internal methodology of Agritec Ltd., based on the norm (Czech Office for Standards, Metrology and Testing, 2011). The content of total fat ranged from 36.22 % to 46.35 %, that of ALA from 1.10 % to 65.20 %, and that of linoleic acid (LA) from 11.10 % to 75.00 % in the analyzed seed samples within all groups. The genotypes were divided also according to the seed color and a linear correlation between all three parameters within these groups was observed. Negative linear dependence was confirmed between parameters; ALA and LA content in the groups: high ALA brown seed (p < 0.0001; correlation coefficient (r) = −0.70), and high ALA yellow seed (p < 0.001; r = −0.36). Also, positive linear dependence between the total fat and the LA content in the groups: low ALA brown seed (p < 0.001; r = 0.34); low ALA yellow seed (p < 0.0001; r = 0.62), was found.

scholarly journals AFLP and SSR markers linked to the yellow seed colour gene in Brassica juncea L.

2011 ◽  
Vol 47 (No. 4) ◽  
pp. 149-155
Author(s):  
Z. Huang ◽  
Y. Zhang ◽  
H.Q. Li ◽  
L. Yang ◽  
Y.Y. Ban ◽  
...  
Keyword(s):  
Brassica Juncea ◽  
Ssr Markers ◽  
Single Gene ◽  
Recessive Allele ◽  
Erucic Acid Content ◽  
Sequence Information ◽  
Seed Colour ◽  
Yellow Seed ◽  
Yellow Mustard ◽  
Colour Gene

Yellow mustard, cultivated in northern Shaanxi of China, is a valuable germplasm of Brassica juncea with low erucic acid content. Its yellow seed colour is controlled by a recessive allele of a single gene, whose dominant allele conditions brown seed colour. To map the yellow seed colour allele, amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) technologies were used to identify markers linked to the recessive allele. The analysis was done on 386 F<sub>2</sub> plants, segregating for seed colour, from the cross Wuqi yellow mustard &times; Wugong mustard. The plants were selfed to determine their seed colour genotype. Twenty AFLP markers and eight SSR markers were identified from 256 AFLP primer combinations and 624 pairs of SSR primers, respectively. Blast analysis indicated that the sequences of four closely linked AFLP and SSR markers showed good collinearity with those of Arabidopsis chromosome 3, and the homologue of the yellow seed colour allele was located between At3g14190 and At3g32130. Sequence information of the region between the two genes of Arabidopsis could be used to develop more closely linked markers to narrow down the homologue of the yellow seed colour allele. These results would accelerate the procedure of yellow seed colour gene cloning and marker-assisted selection for yellow mustard.&nbsp;

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