scholarly journals Identification of SSR markers closely linked to the yellow seed coat color gene in heading Chinese cabbage (Brassica rapaL. ssp.pekinensis)

Biology Open ◽  
2017 ◽  
Vol 6 (2) ◽  
pp. 278-282 ◽  
Author(s):  
Yanjing Ren ◽  
Junqing Wu ◽  
Jing Zhao ◽  
Lingyu Hao ◽  
Lugang Zhang
Keyword(s):  
Ssr Markers ◽  
Chinese Cabbage ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Coat Color ◽  
Yellow Seed ◽  
Heading Chinese Cabbage ◽  
Yellow Seed Coat ◽  
Coat Color Gene ◽  
Color Gene

scholarly journals Mapping and identification of yellow seed coat color genes in Brassica juncea L.

2020 ◽  
Author(s):  
Zhen Huang ◽  
Yang Wang ◽  
Hong Lu ◽  
Xiang Liu ◽  
Lu Liu ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Seed Coat Color ◽  
Flavonoid Pathway ◽  
Yellow Seed ◽  
Color Formation ◽  
Yellow Seed Coat

Abstract BackgroundYellow seed breeding is an effective method to improve the oil content in rapeseed. Yellow seed coat color formation is influenced by various factors, and no clear mechanisms are known. In this study, Bulked segregant RNA-Seq (BSR-Seq) of BC9 population of Wuqi mustard (yellow seed) and Wugong mustard (brown seed) was used to identity the candidate genes controlling the yellow seed color in Brassica juncea L.ResultsYellow seed coat color gene was mapped to chromosome A09, and differentially expressed genes (DEGs) between brown and yellow bulks enriched in the flavonoid pathway. A significant correlation between the expression of BjF3H and BjTT5 and the content of the seed coat color related indexes was identified. Two intron polymorphism (IP) markers linked to the target gene were developed around BjF3H. Therefore, BjF3H was considered as the candidate gene. The BjF3H coding sequences (CDS) of Wuqi mustard and Wugong mustard are 1071-1077bp, encoding protein of 356-358 amino acids. One amino acid change (254, F/V) was identified in the conserved domain. This mutation site was detected in four Brassica rapa (B. rapa) and six Brassica juncea (B. juncea) lines, but not in Brassica napus (B. napus).ConclusionsThe results indicated BjF3H is a candidate gene that related to yellow seed coat color formation in Brassica juncea and provided a comprehensive understanding of the yellow seed coat color mechanism.

scholarly journals Fine-Mapping And Identification of A Candidate Gene Controlling Seed Coat Color In Melon (Cucumis Melo L. Var. Chinensis Pangalo)

2021 ◽  
Author(s):  
Zhicheng Hu ◽  
Xueyin Shi ◽  
Xuemiao Chen ◽  
Jing Zheng ◽  
Aiai Zhang ◽  
...  
Keyword(s):  
Candidate Gene ◽  
Seed Coat ◽  
Coat Color ◽  
Bulked Segregant Analysis ◽  
Dominant Gene ◽  
Seed Coat Color ◽  
Gene Encoding ◽  
Yellow Seed ◽  
Homeobox Protein ◽  
Yellow Seed Coat

Abstract Seed coat color is related to flavonoid content which is closely related to seed dormancy. According to the genetic analysis of a six-generation population derived from two parents (IC2508 with a yellow seed coat and IC2518 with a brown seed coat), we discovered that the yellow seed coat trait in melon was controlled by a single dominant gene, named CmBS-1. Bulked segregant analysis sequencing (BSA-Seq) revealed that the gene was located at 11,860,000–15,890,000 bp (4.03 Mb) on Chr 6. The F2 population was genotyped using insertion-deletions (InDels), from which cleaved amplified polymorphic sequence (dCAPS) markers were derived to construct a genetic map. The gene was then fine-mapped to a 233.98 kb region containing 12 genes. Based on gene sequence analysis with two parents, we found that the MELO3C019554 gene encoding a homeobox protein (PHD transcription factor) had a nonsynonymous single nucleotide polymorphism (SNP) mutation in the coding sequence (CDS), and the SNP mutation resulted in the conversion of an amino acid (A→T) at residue 534. In addition, MELO3C019554 exhibited lower relative expression levels in the yellow seed coat than in the brown seed coat. Furthermore, we found that MELO3C019554 was related to 12 flavonoid metabolites. Thus, we predicted that MELO3C019554 is a candidate gene controlling seed coat color in melon. The study lays a foundation for further cloning projects and functional analysis of this gene, as well as marker-assisted selection breeding.

scholarly journals Comparative transcriptome and flavonoids components analysis reveal the structural genes responsible for the yellow seed coat color of Brassica rapa L.

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e10770
Author(s):  
Yanjing Ren ◽  
Ning Zhang ◽  
Ru Li ◽  
Xiaomin Ma ◽  
Lugang Zhang
Keyword(s):  
Brassica Rapa ◽  
Seed Coat ◽  
Biosynthetic Pathway ◽  
Coat Color ◽  
Seed Coat Color ◽  
Rna Seq ◽  
Yellow Seed ◽  
Color Formation ◽  
Flavonoid Biosynthetic Pathway ◽  
Yellow Seed Coat

Background Seed coat color is an important horticultural trait in Brassica crops, which is divided into two categories: brown/black and yellow. Seeds with yellow seed coat color have higher oil quality, higher protein content and lower fiber content. Yellow seed coat color is therefore considered a desirable trait in hybrid breeding of Brassica rapa, Brassica juncea and Brassica napus. Methods Comprehensive analysis of the abundance transcripts for seed coat color at three development stages by RNA-sequencing (RNA-seq) and corresponding flavonoids compounds by liquid chromatography-tandem mass spectrometry (LC-MS/MS) were carried out in B. rapa. Results We identified 41,286 unigenes with 4,989 differentially expressed genes between brown seeds (B147) and yellow seeds (B80) at the same development stage. Kyoto Encyclopedia of Genes and Genomes enrichment analysis identified 19 unigenes associated with the phenylpropanoid, flavonoid, flavone and flavonol biosynthetic pathways as involved in seed coat color formation. Interestingly, expression levels of early biosynthetic genes (BrCHS, BrCHI, BrF3H, BrF3’H and BrFLS) in the flavonoid biosynthetic pathway were down-regulated while late biosynthetic genes (BrDFR, BrLDOX and BrBAN) were hardly or not expressed in seeds of B80. At the same time, BrTT8 and BrMYB5 were down-regulated in B80. Results of LC-MS also showed that epicatechin was not detected in seeds of B80. We validated the accuracy of our RNA-seq data by RT-qPCR of nine critical genes. Epicatechin was not detected in seeds of B80 by LC-MS/MS. Conclusions The expression levels of flavonoid biosynthetic pathway genes and the relative content of flavonoid biosynthetic pathway metabolites clearly explained yellow seed color formation in B. rapa. This study provides a foundation for further research on the molecular mechanism of seed coat color formation.

scholarly journals Fine Mapping and Whole-Genome Resequencing Identify the Seed Coat Color Gene in Brassica rapa

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0166464 ◽  
Author(s):  
Yanhua Wang ◽  
Lu Xiao ◽  
Shaomin Guo ◽  
Fengyun An ◽  
Dezhi Du
Keyword(s):  
Brassica Rapa ◽  
Fine Mapping ◽  
Seed Coat ◽  
Coat Color ◽  
Whole Genome ◽  
Seed Coat Color ◽  
Genome Resequencing ◽  
Whole Genome Resequencing ◽  
Coat Color Gene ◽  
Color Gene

Generation and mapping of SCAR and CAPS markers linked to the seed coat color gene in Brassica napus using a genome-walking technique

Genome ◽  
2007 ◽  
Vol 50 (7) ◽  
pp. 611-618 ◽  
Author(s):  
Shushu Xiao ◽  
Jinsong Xu ◽  
Yuan Li ◽  
Lei Zhang ◽  
Shijun Shi ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Seed Coat ◽  
Coat Color ◽  
Aflp Markers ◽  
Seed Color ◽  
Seed Coat Color ◽  
A Genome ◽  
Reference Map ◽  
Coat Color Gene ◽  
Color Gene

The yellow seed coat trait in No. 2127-17, a resynthesized purely yellow Brassica napus line, is controlled by a single partially dominant gene, Y. A double-haploid population derived from the F1 of No. 2127-17 × ‘ZY821’ was used to map the seed coat color phenotype. A combination of AFLP analysis and bulked segregant analysis identified 18 AFLP markers linked to the seed coat color trait. The 18 AFLP markers were mapped to a chromosomal region of 37.0 cM with an average of 2.0 cM between adjacent markers. Two markers, AFLP-K and AFLP-H, bracketed the Y locus in an interval of 1.0 cM, such that each was 0.5 cM away from the Y locus. Two other markers, AFLP-A and AFLP-B, co-segregated with the seed color gene. For ease of use in breeding programs, these 4 most tightly linked AFLP markers were converted into reliable PCR-based markers. SCAR-K, which was derived from AFLP-K, was assigned to linkage group 9 (N9) of a B. napus reference map consisting of 150 commonly used SSR (simple sequence repeat) markers. Furthermore, 2 SSR markers (Na14-E08 and Na10-B07) linked to SCAR-K on the reference map were reversely mapped to the linkage map constructed in this study, and also showed linkage to the Y locus. These linked markers would be useful for the transfer of the dominant allele Y from No. 2127-17 to elite cultivars using a marker-assisted selection strategy and would accelerate the cloning of the seed coat color gene.

Verification of Soybean Seed Coat Colour Specific Markers Reveals I Loci Specific Markers Capable for Distinguishing Genotypes Differing in Seed Coated Colour

2020 ◽  
Author(s):  
R. B. Shingare ◽  
V. P. Chimote ◽  
M. P. Deshmukh ◽  
T. J. Bhor ◽  
A. A. Kale
Keyword(s):  
Seed Coat ◽  
Coat Color ◽  
Soybean Seed ◽  
Coat Colour ◽  
Specific Primers ◽  
Seed Coat Colour ◽  
Yellow Seed ◽  
Black Seed ◽  
Soybean Seed Coat ◽  
Yellow Seed Coat

Background: In soybean yellow seed coat is preferred in the market, however, colored ones are currently gaining attention because of their medicinal and nutritive values; besides. Hence it is essential to breed varieties with desired seed coat colour. Methods: Twelve genotypes with six each having yellow and black seed coats were screened with fourteen primers linked to seed coat colour governing loci. Result: Out of them twelve primers showed polymorphism. Monomorphism was observed with both T loci specific and two of the three R loci specific primers. However I locus specific primers i.e. SM303, SM305 and TR showed polymorphism shared by their seed coat color. SM303 amplified a 180 bp sized band in yellow seed coated genotypes and a 130 bp band in black seed coated genotypes. SM305 amplified dual bands with a 200bp band being monomorphic and an additional band (192-216 bp range) present in only yellow seed coated genotypes, of which a 208 bp band was shared by four yellow seed coated genotypes. Cold induced seed coat discoloration specific TR primer generated bands of different size ranges in yellow seed coated (336-344 bp) and black seed coated genotypes (300-320), of which a 340 bp band was shared by four yellow seed coated genotypes.

Inheritance of seed coat color of Ethiopian mustard (Brassica carinata A. Braun)

2010 ◽  
Vol 90 (3) ◽  
pp. 279-281 ◽  
Author(s):  
M Rahman ◽  
M Tahir
Keyword(s):  
Seed Coat ◽  
Coat Color ◽  
Brassica Carinata ◽  
Seed Coat Color ◽  
Yellow Light ◽  
Ethiopian Mustard ◽  
Light Yellow ◽  
Inheritance Model ◽  
Pure Lines ◽  
Color Gene

The inheritance of seed coat color was investigated in Brassica carinata in F1, F2, F3 and backcross progenies of crosses between brown- and yellow-seeded pure lines. Seed coat color in B. carinata was not influenced by xenia effect. Segregation pattern followed a mono-genic incomplete dominance inheritance model The occurrence of yellow/light yellow-brown seed trait in B. carinata may be due to an interaction between brown seed coat color gene and dominant repressor (Rp) genes. Key words: Brassica carinata, inheritance, seed coat color, dominant repressor

INHERITANCE OF SEED COAT COLOR IN BRASSICA JUNCEA

1979 ◽  
Vol 59 (3) ◽  
pp. 635-637 ◽  
Author(s):  
C. L. VERA ◽  
D. L. WOODS ◽  
R. K. DOWNEY
Keyword(s):  
Brassica Juncea ◽  
Seed Coat ◽  
Coat Color ◽  
Seed Color ◽  
Dominant Allele ◽  
Seed Coat Color ◽  
Yellow Seed ◽  
Brown Color

The genetics of seed coat color inheritance in Brassica juncea (L.) Coss. were studied. It was concluded that this character is controlled by two duplicate pairs of genes (R1, R2) for brown color, either of which can produce brown seed color when a single dominant allele is present. Yellow seed results when all alleles at both loci are recessive.

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