scholarly journals Insect Resistance in Transgenic Chrysanthemum [Dendranthema ×grandiflorum (Ramat.) Kitamura] by the Introduction of a Modified δ-endotoxin Gene of Bacillus thuringiensis

2003 ◽  
Vol 53 (4) ◽  
pp. 359-367 ◽  
Author(s):  
Harue Shinoyama ◽  
Atsushi Mochizuki ◽  
Masayasu Komano ◽  
Yukio Nomura ◽  
Teruyuki Nagai
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Resistance ◽  
Dendranthema Grandiflorum ◽  
Transgenic Chrysanthemum

scholarly journals Evolution of Insect Resistance to Bacillus thuringiensis-Transformed Plants

Science ◽  
1996 ◽  
Vol 273 (5280) ◽  
pp. 1412-1413 ◽  
Author(s):  
A. R. Ives; ◽  
D. N. Alstad ◽  
D. A. Andow
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Resistance ◽  
Transformed Plants

Mechanism of insect resistance to the microbial insecticide Bacillus thuringiensis

Science ◽  
1990 ◽  
Vol 247 (4938) ◽  
pp. 72-74 ◽  
Author(s):  
J Van Rie ◽  
W. McGaughey ◽  
D. Johnson ◽  
B. Barnett ◽  
H Van Mellaert
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Resistance ◽  
Microbial Insecticide

scholarly journals An ABC Transporter Mutation Is Correlated with Insect Resistance to Bacillus thuringiensis Cry1Ac Toxin

PLoS Genetics ◽  
2010 ◽  
Vol 6 (12) ◽  
pp. e1001248 ◽  
Author(s):  
Linda J. Gahan ◽  
Yannick Pauchet ◽  
Heiko Vogel ◽  
David G. Heckel
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Resistance ◽  
Abc Transporter ◽  
Cry1ac Toxin

scholarly journals Research and development of genetically engineered soybean using insect-resistance genes derived from Bacillus thuringiensis

2020 ◽  
Vol 18 (1) ◽  
pp. 1-21
Author(s):  
Le Thi Thu Hien ◽  
Pham Le Bich Hang ◽  
Nguyen Tuong Van ◽  
Le Thi Minh Thanh ◽  
Dao Thi Hang ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Resistance ◽  
Resistance Genes ◽  
Insect Pests ◽  
Economic Value ◽  
Herbicide Tolerance ◽  
Genetically Engineered ◽  
Pests And Diseases ◽  
Good Ability ◽  
The World

Soybean (Glycine max) is one of the crops which have high economic value and serve for food, feed and process of many countries around the world. However, there are many factors affecting the productivity of soybean, of which insect pests and diseases are the most harmful agents. Therefore, an application of biotechnology to transfer insect resistance genes derived from a species of bacteria Bacillus thuringiensis can contribute to increase soybean yield and significantly reducing pesticide use. Currently, there are many insecticidal proteins detected from B. thuringiensis such as Cry, Cyt and Vip with a broad and specific spectrum belonged to several orders Lepidoptera, Diptera, Coleoptera, Homopera, and Nematoda. Numerous studies have been implemented over the world to transfer genes encoding these proteins in combination or modified forms to increase their toxicity. Several events of genetically engineered soybean with stacked traits of insect resistance and herbicide tolerance are commercialized and approved to be cultured in many countries such as MON 87701 × MON 89788 or DAS-81419-2. In Vietnam, studies on genetically engineered soybean with insect resistance trait has been carried out. Moreover, the exploitation, screening and selection of high biodiversity and indigenous B. thuringiensis strains which habors specific genes capable of killing targeted insects and serve as materials for plant transformation are great scientific meaning and potential practical application. This will be an important source of materials to create many soybean cultivars with good ability of insect resistance in order to meet specific needs.

scholarly journals MAPK-activated transcription factor PxJun suppresses PxABCB1 expression and confers resistance to Bacillus thuringiensis Cry1Ac toxin in Plutella xylostella (L.)

2021 ◽  
Author(s):  
Jianying Qin ◽  
Le Guo ◽  
Fan Ye ◽  
Shi Kang ◽  
Dan Sun ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Transcriptional Regulation ◽  
Bacillus Thuringiensis ◽  
Insect Resistance ◽  
Plutella Xylostella ◽  
Molecular Basis ◽  
Luciferase Reporter ◽  
Regulation Mechanism ◽  
Cry Toxins ◽  
Regulation Mechanisms

Deciphering the molecular mechanisms underlying insect resistance to Cry toxins produced by Bacillus thuringiensis (Bt) is pivotal for the sustainable utilization of Bt biopesticides and transgenic Bt crops. Previously, we identified that MAPK-mediated reduced expression of the PxABCB1 gene is associated with Bt Cry1Ac resistance in the diamondback moth, Plutella xylostella (L.). However, the underlying transcriptional regulation mechanism remains enigmatic. Herein, the PxABCB1 promoter in Cry1Ac-susceptible and Cry1Ac-resistant P. xylostella strains was cloned and analyzed and found to contain a putative Jun binding site (JBS). A dual-luciferase reporter assay and yeast one-hybrid assay (Y1H) demonstrated that the transcription factor PxJun repressed PxABCB1 expression by interacting with this JBS. The expression levels of PxJun were increased in the midguts of all resistant strains compared to the susceptible strain. Silencing of PxJun expression significantly elevated PxABCB1 expression and Cry1Ac susceptibility in the resistant NIL-R strain, and silencing of PxMAP4K4 expression decreased PxJun expression and also increased PxABCB1 expression. These results indicate that MAPK-activated PxJun suppresses PxABCB1 expression to confer Cry1Ac resistance in P. xylostella, deepening our understanding of the transcriptional regulation of midgut Cry receptor genes and the molecular basis of insect resistance to Bt Cry toxins. Importance The transcriptional regulation mechanisms underlying reduced expression of Bt toxin receptor genes in Bt-resistant insects remain elusive. This study unveils that a transcription factor PxJun activated by the MAPK signaling pathway represses PxABCB1 expression and confers Cry1Ac resistance in P. xylostella. Our results provide new insights into the transcriptional regulation mechanisms of midgut Cry receptor genes and deepen our understanding of the molecular basis of insect resistance to Bt Cry toxins. To our knowledge, this study identified the first transcription factor that can be involved in the transcriptional regulation mechanisms of midgut Cry receptor genes in Bt-resistant insects.

scholarly journals EVOLUTIONARY TRADE-OFFS OF INSECT RESISTANCE TO BACILLUS THURINGIENSIS CROPS: FITNESS COST AFFECTING PATERNITY

Evolution ◽  
2005 ◽  
Vol 59 (4) ◽  
pp. 915-920 ◽  
Author(s):  
Dawn M. Higginson ◽  
Shai Morin ◽  
Megan E. Nyboer ◽  
Robert W. Biggs ◽  
Bruce E. Tabashnik ◽  
...  
Keyword(s):  
Bacillus Thuringiensis ◽  
Insect Resistance ◽  
Fitness Cost ◽  
Trade Offs
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