scholarly journals Identification of the Male Parents of Half-sib Progeny from Japanese Black Pine (Pinus thunbergii Parl.) Clonal Seed Orchard using RAPD Markers

2002 ◽  
Vol 52 (2) ◽  
pp. 71-77 ◽  
Author(s):  
Susumu Goto ◽  
Fumihiko Miyahara ◽  
Yuji Ide
2002 ◽  
Vol 32 (6) ◽  
pp. 983-988 ◽  
Author(s):  
S Goto ◽  
F Miyahara ◽  
Y Ide

Overall male reproductive success was investigated in a Japanese black pine (Pinus thunbergii Parl.) clonal seed orchard consisting of 16 nematode-resistant clones, using random amplified polymorphic DNA (RAPD) markers. We genotyped all 16 of the orchard clones at selected RAPD loci by analyzing diploid needles and 10 haploid megagametophytes from each of them. In addition, the male parent of open-pollinated seedlings was identified by comparing their phenotypes with the genotypes of the orchard clones. In this way the male parents of 559 seedlings of 648 seedlings of progenies from 14 parental (orchard) clones were determined. The level of external pollen contamination in the seed orchard was estimated to be 2.2% for the total population. The effective mean selfing rate observed was 1.5%. Male reproductive success among clones varied widely, from 0.8 to 24.4%, for the total population. Our data suggest that the significant deviation in male reproductive success of individual clones from panmictic ratios may be a problem for the genetic quality in seed orchard crops. To improve the parental balance of seed orchard crops, the use of artificial management techniques such as gibberellin A4/7 treatment, supplemental mass pollination, and rearrangement of clonal ramets is recommended in seed orchards.


2000 ◽  
Vol 100 (3-4) ◽  
pp. 391-395 ◽  
Author(s):  
T. Kondo ◽  
K. Terada ◽  
E. Hayashi ◽  
N. Kuramoto ◽  
M. Okamura ◽  
...  

2015 ◽  
Vol 69 (4) ◽  
pp. 366-368
Author(s):  
Naoki Negishi ◽  
Nobuaki Urata ◽  
Katsuhiko Nakahama ◽  
Akiyoshi Kawaoka

2020 ◽  
Vol 25 (2) ◽  
pp. 76-82 ◽  
Author(s):  
Saki Fujita ◽  
Hanami Watanabe ◽  
Vitas Marozas ◽  
Yutaka Tamai ◽  
Fuyuki Satoh ◽  
...  

2014 ◽  
Vol 30 (4) ◽  
pp. 383-392
Author(s):  
Yukiyoshi Teramoto ◽  
Etsuro Shimokawa ◽  
Tsugio Ezaki ◽  
Kun-Woo Chun ◽  
Suk-Woo Kim ◽  
...  

Sign in / Sign up

Export Citation Format

Share Document