scholarly journals In Vivo Experiment on the Metabolism of Cesium in Human Blood with Reference to Rubidium and Potassium

1966 ◽  
Vol 7 (1) ◽  
pp. 29-46 ◽  
Author(s):  
Noboru YAMAGATA ◽  
Kiyoshi IWASHIMA ◽  
Teruo NAGAI ◽  
Kazuo WATARI ◽  
Takeshi A. IINUMA
Keyword(s):  
Human Blood ◽  
In Vivo Experiment

scholarly journals An in vivo model allowing continuous observation of human vascular formation in the same animal over time

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Yohei Tsukada ◽  
Fumitaka Muramatsu ◽  
Yumiko Hayashi ◽  
Chiaki Inagaki ◽  
Hang Su ◽  
...  
Keyword(s):  
Blood Vessel ◽  
Blood Vessels ◽  
Human Blood ◽  
Molecular Mechanisms ◽  
Human Tumor ◽  
Continuous Observation ◽  
In Vivo Models ◽  
Cranial Window ◽  
Pathological Conditions

AbstractAngiogenesis contributes to numerous pathological conditions. Understanding the molecular mechanisms of angiogenesis will offer new therapeutic opportunities. Several experimental in vivo models that better represent the pathological conditions have been generated for this purpose in mice, but it is difficult to translate results from mouse to human blood vessels. To understand human vascular biology and translate findings into human research, we need human blood vessel models to replicate human vascular physiology. Here, we show that human tumor tissue transplantation into a cranial window enables engraftment of human blood vessels in mice. An in vivo imaging technique using two-photon microscopy allows continuous observation of human blood vessels until at least 49 days after tumor transplantation. These human blood vessels make connections with mouse blood vessels as shown by the finding that lectin injected into the mouse tail vein reaches the human blood vessels. Finally, this model revealed that formation and/or maintenance of human blood vessels depends on VEGFR2 signaling. This approach represents a useful tool to study molecular mechanisms of human blood vessel formation and to test effects of drugs that target human blood vessels in vivo to show proof of concept in a preclinical model.

The Dependence of the Mutagenic Effect on the Dose of X-Ray Irradiation in an In Vivo Experiment on Female (CBA×C57Bl/6)F1 Mice

2018 ◽  
Vol 166 (1) ◽  
pp. 43-45 ◽  
Author(s):  
L. P. Sycheva ◽  
R. A. Shchegoleva ◽  
N. I. Lisina ◽  
A. V. Gordeev ◽  
L. M. Rozhdestvenskii
Keyword(s):  
Mutagenic Effect ◽  
X Ray ◽  
In Vivo Experiment

scholarly journals The Effects of miR-195-5p/MMP14 on Proliferation and Invasion of Cervical Carcinoma Cells Through TNF Signaling Pathway Based on Bioinformatics Analysis of Microarray Profiling

2018 ◽  
Vol 50 (4) ◽  
pp. 1398-1413 ◽  
Author(s):  
Min Li ◽  
Chun-Xia Ren ◽  
Jian-Mei Zhang ◽  
Xiao-Yan Xin ◽  
Teng Hua ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Signaling Pathway ◽  
Microarray Analysis ◽  
Cervical Carcinoma ◽  
Carcinoma Cells ◽  
Gene Set Enrichment Analysis ◽  
Qrt Pcr ◽  
In Vivo Experiment ◽  
Proliferation And Invasion

Background/Aims: This study is aimed at identification of miR-195-5p/MMP14 expression in cervical cancer (CC) and their roles on cell proliferation and invasion profile of CC cells through TNF signaling pathway in CC. Methods: Microarray analysis, gene set enrichment analysis (GSEA) and DAVID were used to analyze differentially expressed miRNAs, mRNAs and signaling pathways. MiR-195-5p and MMP14 expression levels in CC cell were determined by qRT-PCR. Western blot was employed to measure MMP14 and TNF signaling pathway-relating protein level. Luciferase reporter system was used to confirm the targeting relationship between MMP14 and miR-195-5p. Cell proliferation and invasion was respectively deeded by CCK8, transwell. In vivo experiment was carried out to study the impact of MMP14 and miR-195-5p on CC development in mice. Results: The microarray analysis and the results of qRT-PCR determined that miR-195-5p was under-expressed and MMP14 was over-expressed in CC cells. GSEA and DAVID analysis showed that TNF signaling pathway was regulated by miR-195-5p/MMP14 and activated in cervical carcinoma cells. The miR-195-5p and MMP14 have a negative regulation relation. In vivo experiment found that down-regulated MMP14 and up-regulated miR-195-5p suppressed the tumor development. Conclusion: Our results suggest that MMP14 is a direct target of miR-195-5p, and down-regulated MMP14 and up-regulated miR-195-5p suppressed proliferation and invasion of CC cells by inhibiting TNF signaling pathway.

scholarly journals No cell is an island: circulating T cell:monocyte complexes are markers of immune perturbations

2018 ◽  
Author(s):  
Julie G. Burel ◽  
Mikhail Pomaznoy ◽  
Cecilia S. Lindestam Arlehamn ◽  
Daniela Weiskopf ◽  
Ricardo da Silva Antunes ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
T Cell ◽  
Immune Responses ◽  
Human Blood ◽  
Significant Proportion ◽  
Complex Frequency ◽  
The Common ◽  
First Time ◽  
High Resolution Microscopy

AbstractOur results highlight for the first time that a significant proportion of cell doublets in flow cytometry, previously believed to be the result of technical artefacts and thus ignored in data acquisition and analysis, are the result of true biological interaction between immune cells. In particular, we show that cell:cell doublets pairing a T cell and a monocyte can be directly isolated from human blood, and high resolution microscopy shows polarized distribution of LFA1/ICAM1 in many doublets, suggesting in vivo formation. Intriguingly, T cell:monocyte complex frequency and phenotype fluctuate with the onset of immune perturbations such as infection or immunization, reflecting expected polarization of immune responses. Overall these data suggest that cell doublets reflecting T cell-monocyte in vivo immune interactions can be detected in human blood and that the common approach in flow cytometry to avoid studying cell:cell complexes should be revisited.

Investigation of the effect of blackcurrant alcohol-based mors in the composition of an alcoholic beverage on the degree of intoxication of laboratory animals with ethyl alcohol

2021 ◽  
pp. 29-31
Author(s):  
Анна Георгиевна Калинина ◽  
Ирина Михайловна Абрамова ◽  
Наталья Евгеньевна Головачёва ◽  
Светлана Семеновна Морозова ◽  
Любовь Павловна Галлямова
Keyword(s):  
Ethyl Alcohol ◽  
Alcoholic Beverage ◽  
Laboratory Animals ◽  
Negative Consequences ◽  
Chronic Intoxication ◽  
In Vivo Experiment

Результаты исследования в опыте in vivo подтвердили снижение негативных последствий при хронической интоксикации животных для образцов настойки сладкой крепостью 20 %, содержащей черносмородиновый спиртованный морс, по сравнению с раствором этилового спирта аналогичной крепости. The results of the study in the in vivo experiment confirmed a reduction in the negative consequences of chronic intoxication of animals for samples of tincture with a sweet strength of 20 %, containing blackcurrant alcoholic mors, compared with a solution of ethyl alcohol of a similar strength.

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