The Antimicrobial and Tissue Healing Efficacy of the Atmospheric Pressure Cold Plasma on Grade III Infected Pressure Ulcer: Randomized Controlled In Vivo Experiment

2020 ◽  
Author(s):  
Ayşe Akbiyik ◽  
Dilek Sari ◽  
Utku Kürşat Ercan ◽  
Yiğit Uyanikgil ◽  
Hüseyin Taşli ◽  
...  
Keyword(s):  
Pressure Ulcer ◽  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Randomized Controlled ◽  
In Vivo Experiment ◽  
Tissue Healing ◽  
Grade Iii

scholarly journals Plasma Medicine: Applications of Cold Atmospheric Pressure Plasma in Dermatology

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Thoralf Bernhardt ◽  
Marie Luise Semmler ◽  
Mirijam Schäfer ◽  
Sander Bekeschus ◽  
Steffen Emmert ◽  
...  
Keyword(s):  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Chronic Wounds ◽  
Atmospheric Pressure Plasma ◽  
Plasma Jet ◽  
Great Promise ◽  
Plasma Medicine ◽  
Positive Effects ◽  
Pressure Plasma

The ability to produce cold plasma at atmospheric pressure conditions was the basis for the rapid growth of plasma-related application areas in biomedicine. Plasma comprises a multitude of active components such as charged particles, electric current, UV radiation, and reactive gas species which can act synergistically. Anti-itch, antimicrobial, anti-inflammatory, tissue-stimulating, blood flow-enhancing, and proapoptotic effects were demonstrated in in vivo and in vitro experiments, and until now, no resistance of pathogens against plasma treatment was observed. The combination of the different active agents and their broad range of positive effects on various diseases, especially easily accessible skin diseases, renders plasma quite attractive for applications in medicine. For medical applications, two different types of cold plasma appear suitable: indirect (plasma jet) and direct (dielectric barrier discharge—DBD) plasma sources. The DBD device PlasmaDerm® VU-2010 (CINOGY Technologies GmbH), the atmospheric pressure plasma jet (APPJ) kINPen® MED (INP Greifswald/neoplas tools GmbH), and the SteriPlas (Adtec Ltd., London, United Kingdom) are CE-certified as a medical product to treat chronic wounds in humans and showed efficacy and a good tolerability. Recently, the use of plasma in cancer research and oncology is of particular interest. Plasma has been shown to induce proapoptotic effects more efficiently in tumor cells compared with the benign counterparts, leads to cellular senescence, and—as shown in vivo—reduces skin tumors. To this end, a world-wide first Leibniz professorship for plasmabiotechnology in dermatology has been introduced to establish a scientific network for the investigation of the efficacy and safety of cold atmospheric plasma in dermatooncology. Hence, plasma medicine especially in dermatology holds great promise.

High voltage pulsed current in pressure ulcer healing: results of a randomized, controlled trial – preliminary study

Leczenie Ran ◽  
2013 ◽  
Vol 10 (1) ◽  
pp. 13-22 ◽  
Author(s):  
Anna Polak ◽  
Anna Walczak ◽  
Jakub Taradaj ◽  
Marzena Dzikiewicz ◽  
Aldona Augustak ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Pressure Ulcer ◽  
High Voltage ◽  
Ulcer Healing ◽  
Controlled Trial ◽  
Pulsed Current ◽  
Randomized Controlled ◽  
Preliminary Study

A randomized controlled trial comparing autologous radiolabeled in vivo platelet (PLT) recoveries and survivals of 7-day-stored PLT-rich plasma and buffy coat PLTs from the same subjects

Transfusion ◽  
2011 ◽  
Vol 51 (6) ◽  
pp. 1241-1248 ◽  
Author(s):  
Larry J. Dumont ◽  
Deborah F. Dumont ◽  
Zoe M. Unger ◽  
Alan Siegel ◽  
Zbigniew M. Szczepiorkowski ◽  
...  
Keyword(s):  
Randomized Controlled Trial ◽  
Controlled Trial ◽  
Buffy Coat ◽  
Randomized Controlled

scholarly journals Electrical Supply Circuit for a Cold Plasma Source at Atmospheric Pressure Based on a Voltage Multiplier

Polymers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 2132
Author(s):  
Ovidiu S. Stoican
Keyword(s):  
Atmospheric Pressure ◽  
Cold Plasma ◽  
Plasma Source ◽  
Electrical Circuit ◽  
Jet Formation ◽  
Voltage Multiplier ◽  
Voltage Output ◽  
Intermediate Output ◽  
Electrical Supply ◽  
Lower Voltage

A cold plasma source operating at atmospheric pressure powered by a voltage multiplier is reported. In addition to its usual high voltage output, there is an intermediate output of lower voltage and higher current capability. A discharge current is drawn from both outputs. The ratio of the current supplied by each output depends on the operating state, namely, before or after the plasma jet formation. The electrical circuit is equivalent to two dc sources connected in parallel, used to initiate and sustain the electrical discharge. The plasma source is aimed to study the effect of cold plasma on the surface of various liquid or solid materials, including polymers.

Cold Plasma Treatment Promotes Full-thickness Healing of Skin Wounds in Murine Models

2021 ◽  
pp. 153473462110021
Author(s):  
Joon M. Jung ◽  
Hae K. Yoon ◽  
Chang J. Jung ◽  
Soo Y. Jo ◽  
Sang G. Hwang ◽  
...  
Keyword(s):  
Wound Healing ◽  
Plasma Treatment ◽  
Cold Plasma ◽  
Smooth Muscle Actin ◽  
Treated Group ◽  
Control Group ◽  
Alpha Smooth Muscle Actin ◽  
Skin Wound Healing

Cold plasma can be beneficial for promoting skin wound healing and has a high potential of being effectively used in treating various wounds. Our aim was to verify the effect of cold plasma in accelerating wound healing and investigate its underlying mechanism in vitro and in vivo. For the in vivo experiments, 2 full-thickness dermal wounds were created in each mouse (n = 30). While one wound was exposed to 2 daily plasma treatments for 3 min, the other wound served as a control. The wounds were evaluated by imaging and histological analyses at 4, 7, and 11 days post the wound infliction process. Immunohistochemical studies were also performed at the same time points. In vitro proliferation and scratch assay using HaCaT keratinocytes and fibroblasts were performed. The expression levels of wound healing–related genes were analyzed by real-time polymerase chain reaction and western blot analysis. On day 7, the wound healing rates were 53.94% and 63.58% for the control group and the plasma-treated group, respectively. On day 11, these rates were 76.05% and 93.44% for the control and plasma-treated groups, respectively, and the difference between them was significant ( P = .039). Histological analysis demonstrated that plasma treatment promotes the formation of epidermal keratin and granular layers. Immunohistochemical studies also revealed that collagen 1, collagen 3, and alpha-smooth muscle actin appeared more abundantly in the plasma-treated group than in the control group. In vitro, the proliferation of keratinocytes was promoted by plasma exposure. Scratch assay showed that fibroblast exposure to plasma increased their migration. The expression levels of collagen 1, collagen 3, and alpha-smooth muscle actin were elevated upon plasma treatment. In conclusion, cold plasma can accelerate skin wound healing and is well tolerated.

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