scholarly journals Time-Lapse Photographic Studies of Pedigrees of X-Irradiated HeLa Cells

1973 ◽  
Vol 14 (3) ◽  
pp. 248-257 ◽  
Author(s):  
H. SASAKI
Keyword(s):  
Hela Cells ◽  
Time Lapse ◽  
Photographic Studies

scholarly journals Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hiroaki Shimono ◽  
Atsushi Kaida ◽  
Hisao Homma ◽  
Hitomi Nojima ◽  
Yusuke Onozato ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Time Lapse ◽  
S Phase ◽  
G1 Phase ◽  
M Phase ◽  
G2 Phase ◽  
The Difference ◽  
Time Lapse Imaging ◽  
First Time

AbstractIn this study, we examined the fluctuation in radioresponse of HeLa cells during the cell cycle. For this purpose, we used HeLa cells expressing two types of fluorescent ubiquitination-based cell cycle indicators (Fucci), HeLa-Fucci (CA)2 and HeLa-Fucci (SA), and combined this approach with the micronucleus (MN) assay to assess radioresponse. The Fucci system distinguishes cell cycle phases based on the colour of fluorescence and cell morphology under live conditions. Time-lapse imaging allowed us to further identify sub-positions within the G1 and S phases at the time of irradiation by two independent means, and to quantitate the number of MNs by following each cell through M phase until the next G1 phase. Notably, we found that radioresponse was low in late G1 phase, but rapidly increased in early S phase. It then decreased until late S phase and increased in G2 phase. For the first time, we demonstrated the unique fluctuation of radioresponse by the MN assay during the cell cycle in HeLa cells. We discuss the difference between previous clonogenic experiments using M phase-synchronised cell populations and ours, as well as the clinical implications of the present findings.

scholarly journals Automated Analysis of Time-Lapse Imaging of Nuclear Translocation by Retrospective Strategy and Its Application to STAT1 in HeLa Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e27454 ◽  
Author(s):  
Fujun Han ◽  
Peizhou Liang ◽  
Feifei Wang ◽  
Lingyun Zeng ◽  
Biliang Zhang
Keyword(s):  
Hela Cells ◽  
Nuclear Translocation ◽  
Automated Analysis ◽  
Time Lapse ◽  
Time Lapse Imaging

Locomotory invasion of human cervical epithelium and avian fibroblasts by HeLa cells in vitro

1982 ◽  
Vol 57 (1) ◽  
pp. 293-314 ◽  
Author(s):  
E.M. Stephenson
Keyword(s):  
Epithelial Cells ◽  
Hela Cells ◽  
Time Lapse ◽  
Tumour Cells ◽  
Contact Inhibition ◽  
Type I ◽  
Original Direction ◽  
Epithelial Sheet ◽  
Invasion Index

The locomotory invasive ability of HeLa cells was tested against: (a) embryonic chick heart fibroblasts (CHF); and (b) normal epithelial cells from human cervix (HCE) in explant confrontations. Data for analyses were obtained from replicate cultures fixed 24 h after junction and from 24-h time-lapse films. The mean invasion index for HeLa versus CHF did not indicate significant obstruction but analyses of hourly radial advance and orientation frequencies showed that obstruction eventually developed as postjunctional incubation time increased. Early contacts between HeLa and CHF demonstrated non-reciprocity of type I contact inhibition of locomotion by the tumour cells, which continued moving in their original direction to underlap contact-inhibited fibroblasts and eventually to occupy spaces vacated by them. When CHF population density increased and free space diminished, HeLa cells displayed directional and probably substrate-dependent contact inhibition. The high invasion index of HeLa versus HCE was largely due to occupation of previous HCE territory by tumour cells and only occasionally to actual infiltration of the epithelial sheet. After contact with HeLa, ruffling substrate-adherent marginal epithelial cells displayed contractile, type I contact inhibition of locomotion. After orientation changes, they gradually retreated. Against HCE, HeLa cells exhibited non-reciprocity of type I contact inhibition and continued radially forward, following the retreating epithelial margin. They did not move onto exposed upper surfaces of epithelial cells and did not underlap marginal cells firmly adherent to the substratum. Invasion of the epithelial sheet was seen only when initial access beneath a cell with a non-adherent margin was available. The contact relationships of isolated invading HeLa cells with their epithelial neighbours suggested successive non-reciprocal contact inhibition reactions.

scholarly journals Relation between cell activity and the distribution of cytoplasmic actin and myosin.

1981 ◽  
Vol 90 (1) ◽  
pp. 84-91 ◽  
Author(s):  
I M Herman ◽  
N J Crisona ◽  
T D Pollard
Keyword(s):  
Hela Cells ◽  
Experimental Approach ◽  
Cultured Cells ◽  
Cell Activity ◽  
Time Lapse ◽  
Entire Surface ◽  
Cytoplasmic Actin ◽  
Filament Bundles ◽  
Slow Moving ◽  
Diffuse Distribution

We documented the activity of cultured cells on time-lapse videotapes and then stained these identified cells with antibodies to actin and myosin. This experimental approach enabled us to directly correlate cellular activity with the distribution of cytoplasmic actin and myosin. When trypsinized HeLa cells spread onto a glass surface, the cortical cytoplasm was the most actively motile and random, bleb-like extensions (0.5-4.0 micrometer wide, 2-5 micrometer long) occurred over the entire surface until the cells started to spread. During spreading, ruffling membranes were found at the cell perimeter. The actin staining was found alone in the surface blebs and ruffles and together with myosin staining in the cortical cytoplasm at the bases of the blebs and ruffles. In well-spread, stationary HeLa cells most of the actin and myosin was found in stress fibers but there was also diffuse antiactin fluorescence in areas of motile cytoplasm such as leading lamellae and ruffling membranes. Similarly, all 22 of the rapidly translocating embryonic chick cells had only diffuse actin staining. Between these extremes were slow-moving HeLa cells, which had combinations of diffuse and fibrous antiactin and antimyosin staining. These results suggest that large actomyosin filament bundles are associated with nonmotile cytoplasm and that actively motile cytoplasm has a more diffuse distribution of these proteins.

Time-lapse studies on the effect of vincristine on HeLa cells

1981 ◽  
Vol 17 (3) ◽  
pp. 307-319 ◽  
Author(s):  
A.M. Lengsfeld ◽  
B. Schultze ◽  
W. Maurer
Keyword(s):  
Hela Cells ◽  
Time Lapse

Ultra-Violet Damage to Living HeLa Cells as Recorded by Time-Lapse Motion Picture Studies.

1957 ◽  
Vol 95 (3) ◽  
pp. 589-591 ◽  
Author(s):  
P. O. Montgomery ◽  
W. A. Bonner ◽  
F. F. Roberts
Keyword(s):  
Motion Picture ◽  
Hela Cells ◽  
Ultra Violet ◽  
Time Lapse

scholarly journals Visualization of TGN to Endosome Trafficking through Fluorescently Labeled MPR and AP-1 in Living Cells

2003 ◽  
Vol 14 (1) ◽  
pp. 142-155 ◽  
Author(s):  
Satoshi Waguri ◽  
Frédérique Dewitte ◽  
Roland Le Borgne ◽  
Yves Rouillé ◽  
Yasuo Uchiyama ◽  
...  
Keyword(s):  
Hela Cells ◽  
Fluorescent Protein ◽  
Chimeric Protein ◽  
Time Lapse ◽  
Living Cells ◽  
Endocytic Pathway ◽  
Cell Periphery ◽  
Endosome Trafficking ◽  
Early Endosomes ◽  
Green Fluorescent

We have stably expressed in HeLa cells a chimeric protein made of the green fluorescent protein (GFP) fused to the transmembrane and cytoplasmic domains of the mannose 6-phosphate/insulin like growth factor II receptor in order to study its dynamics in living cells. At steady state, the bulk of this chimeric protein (GFP-CI-MPR) localizes to the trans-Golgi network (TGN), but significant amounts are also detected in peripheral, tubulo-vesicular structures and early endosomes as well as at the plasma membrane. Time-lapse videomicroscopy shows that the GFP-CI-MPR is ubiquitously detected in tubular elements that detach from the TGN and move toward the cell periphery, sometimes breaking into smaller tubular fragments. The formation of the TGN-derived tubules is temperature dependent, requires the presence of intact microtubule and actin networks, and is regulated by the ARF-1 GTPase. The TGN-derived tubules fuse with peripheral, tubulo-vesicular structures also containing the GFP-CI-MPR. These structures are highly dynamic, fusing with each other as well as with early endosomes. Time-lapse videomicroscopy performed on HeLa cells coexpressing the CFP-CI-MPR and the AP-1 complex whose γ-subunit was fused to YFP shows that AP-1 is present not only on the TGN and peripheral CFP-CI-MPR containing structures but also on TGN-derived tubules containing the CFP-CI-MPR. The data support the notion that tubular elements can mediate MPR transport from the TGN to a peripheral, tubulo-vesicular network dynamically connected with the endocytic pathway and that the AP-1 coat may facilitate MPR sorting in the TGN and endosomes.

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