scholarly journals A TIME-LAPSE STUDY OF EFFECTS OF ANTICELLULAR ANTIBODY ON MEMBRANE MOBILITY AND PHAGOCYTIC ACTIVITY OF HELA CELLS

1967 ◽  
Vol 33 (3) ◽  
pp. 709-712 ◽  
Author(s):  
Francis J. Carey ◽  
Olive S. Pettengill
Keyword(s):  
Hela Cells ◽  
Phagocytic Activity ◽  
Time Lapse

scholarly journals Phagocytic Activity of HeLa Cells after Thymidine Treatment

1983 ◽  
Vol 46 (4) ◽  
pp. 479-489 ◽  
Author(s):  
Miya KOBAYASHI ◽  
Takeshi HOSHINO
Keyword(s):  
Hela Cells ◽  
Phagocytic Activity

scholarly journals Fluctuation in radioresponse of HeLa cells during the cell cycle evaluated based on micronucleus frequency

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Hiroaki Shimono ◽  
Atsushi Kaida ◽  
Hisao Homma ◽  
Hitomi Nojima ◽  
Yusuke Onozato ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Hela Cells ◽  
Time Lapse ◽  
S Phase ◽  
G1 Phase ◽  
M Phase ◽  
G2 Phase ◽  
The Difference ◽  
Time Lapse Imaging ◽  
First Time

AbstractIn this study, we examined the fluctuation in radioresponse of HeLa cells during the cell cycle. For this purpose, we used HeLa cells expressing two types of fluorescent ubiquitination-based cell cycle indicators (Fucci), HeLa-Fucci (CA)2 and HeLa-Fucci (SA), and combined this approach with the micronucleus (MN) assay to assess radioresponse. The Fucci system distinguishes cell cycle phases based on the colour of fluorescence and cell morphology under live conditions. Time-lapse imaging allowed us to further identify sub-positions within the G1 and S phases at the time of irradiation by two independent means, and to quantitate the number of MNs by following each cell through M phase until the next G1 phase. Notably, we found that radioresponse was low in late G1 phase, but rapidly increased in early S phase. It then decreased until late S phase and increased in G2 phase. For the first time, we demonstrated the unique fluctuation of radioresponse by the MN assay during the cell cycle in HeLa cells. We discuss the difference between previous clonogenic experiments using M phase-synchronised cell populations and ours, as well as the clinical implications of the present findings.

scholarly journals Automated Analysis of Time-Lapse Imaging of Nuclear Translocation by Retrospective Strategy and Its Application to STAT1 in HeLa Cells

PLoS ONE ◽  
2011 ◽  
Vol 6 (11) ◽  
pp. e27454 ◽  
Author(s):  
Fujun Han ◽  
Peizhou Liang ◽  
Feifei Wang ◽  
Lingyun Zeng ◽  
Biliang Zhang
Keyword(s):  
Hela Cells ◽  
Nuclear Translocation ◽  
Automated Analysis ◽  
Time Lapse ◽  
Time Lapse Imaging

Locomotory invasion of human cervical epithelium and avian fibroblasts by HeLa cells in vitro

1982 ◽  
Vol 57 (1) ◽  
pp. 293-314 ◽  
Author(s):  
E.M. Stephenson
Keyword(s):  
Epithelial Cells ◽  
Hela Cells ◽  
Time Lapse ◽  
Tumour Cells ◽  
Contact Inhibition ◽  
Type I ◽  
Original Direction ◽  
Epithelial Sheet ◽  
Invasion Index

The locomotory invasive ability of HeLa cells was tested against: (a) embryonic chick heart fibroblasts (CHF); and (b) normal epithelial cells from human cervix (HCE) in explant confrontations. Data for analyses were obtained from replicate cultures fixed 24 h after junction and from 24-h time-lapse films. The mean invasion index for HeLa versus CHF did not indicate significant obstruction but analyses of hourly radial advance and orientation frequencies showed that obstruction eventually developed as postjunctional incubation time increased. Early contacts between HeLa and CHF demonstrated non-reciprocity of type I contact inhibition of locomotion by the tumour cells, which continued moving in their original direction to underlap contact-inhibited fibroblasts and eventually to occupy spaces vacated by them. When CHF population density increased and free space diminished, HeLa cells displayed directional and probably substrate-dependent contact inhibition. The high invasion index of HeLa versus HCE was largely due to occupation of previous HCE territory by tumour cells and only occasionally to actual infiltration of the epithelial sheet. After contact with HeLa, ruffling substrate-adherent marginal epithelial cells displayed contractile, type I contact inhibition of locomotion. After orientation changes, they gradually retreated. Against HCE, HeLa cells exhibited non-reciprocity of type I contact inhibition and continued radially forward, following the retreating epithelial margin. They did not move onto exposed upper surfaces of epithelial cells and did not underlap marginal cells firmly adherent to the substratum. Invasion of the epithelial sheet was seen only when initial access beneath a cell with a non-adherent margin was available. The contact relationships of isolated invading HeLa cells with their epithelial neighbours suggested successive non-reciprocal contact inhibition reactions.

scholarly journals Relation between cell activity and the distribution of cytoplasmic actin and myosin.

1981 ◽  
Vol 90 (1) ◽  
pp. 84-91 ◽  
Author(s):  
I M Herman ◽  
N J Crisona ◽  
T D Pollard
Keyword(s):  
Hela Cells ◽  
Experimental Approach ◽  
Cultured Cells ◽  
Cell Activity ◽  
Time Lapse ◽  
Entire Surface ◽  
Cytoplasmic Actin ◽  
Filament Bundles ◽  
Slow Moving ◽  
Diffuse Distribution

We documented the activity of cultured cells on time-lapse videotapes and then stained these identified cells with antibodies to actin and myosin. This experimental approach enabled us to directly correlate cellular activity with the distribution of cytoplasmic actin and myosin. When trypsinized HeLa cells spread onto a glass surface, the cortical cytoplasm was the most actively motile and random, bleb-like extensions (0.5-4.0 micrometer wide, 2-5 micrometer long) occurred over the entire surface until the cells started to spread. During spreading, ruffling membranes were found at the cell perimeter. The actin staining was found alone in the surface blebs and ruffles and together with myosin staining in the cortical cytoplasm at the bases of the blebs and ruffles. In well-spread, stationary HeLa cells most of the actin and myosin was found in stress fibers but there was also diffuse antiactin fluorescence in areas of motile cytoplasm such as leading lamellae and ruffling membranes. Similarly, all 22 of the rapidly translocating embryonic chick cells had only diffuse actin staining. Between these extremes were slow-moving HeLa cells, which had combinations of diffuse and fibrous antiactin and antimyosin staining. These results suggest that large actomyosin filament bundles are associated with nonmotile cytoplasm and that actively motile cytoplasm has a more diffuse distribution of these proteins.

scholarly journals Microglial Cell Morphology and Phagocytic Activity Are Critically Regulated by the Neurosteroid Allopregnanolone: A Possible Role in Neuroprotection

Cells ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 698
Author(s):  
Valérie Jolivel ◽  
Susana Brun ◽  
Fabien Binamé ◽  
Jérémie Benyounes ◽  
Omar Taleb ◽  
...  
Keyword(s):  
Neurological Disorders ◽  
Phagocytic Activity ◽  
Microglial Cell ◽  
Neurological Diseases ◽  
Time Lapse ◽  
Microglial Cells ◽  
Primary Microglia ◽  
Gaba A ◽  
Neuroprotective Strategies ◽  
Underlying Mechanisms

Microglial cells are key players in neural pathogenesis and microglial function regulation appears to be pivotal in controlling neuroinflammatory/neurological diseases. Here, we investigated the effects and mechanism of action of neurosteroid allopregnanolone (ALLO) on murine microglial BV-2 cells and primary microglia in order to determine ALLO-induced immunomodulatory potential and to provide new insights for the development of both natural and safe neuroprotective strategies targeting microglia. Indeed, ALLO-treatment is increasingly suggested as beneficial in various models of neurological disorders but the underlying mechanisms have not been elucidated. Therefore, the microglial cells were cultured with various serum concentrations to mimic the blood-brain-barrier rupture and to induce their activation. Proliferation, viability, RT-qPCR, phagocytosis, and morphology analyzes, as well as migration with time-lapse imaging and quantitative morphodynamic methods, were combined to investigate ALLO actions on microglia. BV-2 cells express subunits of GABA-A receptor that mediates ALLO activity. ALLO (10µM) induced microglial cell process extension and decreased migratory capacity. Interestingly, ALLO modulated the phagocytic activity of BV-2 cells and primary microglia. Our results, which show a direct effect of ALLO on microglial morphology and phagocytic function, suggest that the natural neurosteroid-based approach may contribute to developing effective strategies against neurological disorders that are evoked by microglia-related abnormalities.

Time-lapse studies on the effect of vincristine on HeLa cells

1981 ◽  
Vol 17 (3) ◽  
pp. 307-319 ◽  
Author(s):  
A.M. Lengsfeld ◽  
B. Schultze ◽  
W. Maurer
Keyword(s):  
Hela Cells ◽  
Time Lapse

Ultra-Violet Damage to Living HeLa Cells as Recorded by Time-Lapse Motion Picture Studies.

1957 ◽  
Vol 95 (3) ◽  
pp. 589-591 ◽  
Author(s):  
P. O. Montgomery ◽  
W. A. Bonner ◽  
F. F. Roberts
Keyword(s):  
Motion Picture ◽  
Hela Cells ◽  
Ultra Violet ◽  
Time Lapse
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