scholarly journals Modification of Radiosensitivity of Ehrlich Ascites Carcinoma Cells in vivo. Part 1, Some Basic Experiments on the Role of Lethally Irradiated Tumor Cells

1970 ◽  
Vol 11 (2) ◽  
pp. 61-69
Author(s):  
Muneyasu URANO ◽  
Ichiro SHIRAKURA ◽  
Norimoto TANAKA
Keyword(s):  
Tumor Cells ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Ehrlich Ascites ◽  
Basic Experiments

THE ENZYMIC FORMATION OF 6-(METHYLMERCAPTO)PURINE RIBONUCLEOSIDE 5′-PHOSPHATE

1966 ◽  
Vol 44 (2) ◽  
pp. 229-245 ◽  
Author(s):  
Ian C. Caldwell ◽  
J. Frank Henderson ◽  
A. R. P. Paterson
Keyword(s):  
Tumor Cells ◽  
Blood Cells ◽  
Intraperitoneal Injection ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Ehrlich Ascites ◽  
Mouse Tissues ◽  
Enzymatic Methods

6-(Methylmercapto)purine ribonucleoside (Me6MPR) is efficiently phosphorylated in mouse tissues and in Ehrlich ascites carcinoma cells in vivo; tumor cells in vitro and cell-free extracts of the tumor also phosphorylate this analogue ribonucleoside. The product of this reaction has been identified by chemical and enzymatic methods and by its chromatographic behaviour as Me6MPR 5′-phosphate. The evidence presented in this report indicates that no other major metabolites of Me6MPR are formed.The phosphorylation of Me6MPR by cell-free tumor extracts requires ATP and Mn2+ (or Mg2+), and evidence is presented that the reaction is probably mediated by adenosine kinase.Me-14C-6MPR is rapidly taken up by most mouse tissues following its intraperitoneal injection. Forty minutes after injection of the labeled drug, the highest levels of radioactivity were found in intestine, liver, blood cells, lung, and spleen, in descending order; virtually no radioactivity was found in brain tissue or in blood plasma.

Trichosanthes dioica seed lectin inhibits Ehrlich ascites carcinoma cells growth in vivo in mice by inducing G 0 /G 1 cell cycle arrest

2021 ◽  
Author(s):  
Shaikh Shohidul Islam ◽  
Md. Rezaul Karim ◽  
A. K. M. Asaduzzaman ◽  
A. H. M. Khurshid Alam ◽  
Zahid Hayat Mahmud ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cell Cycle Arrest ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Cycle Arrest ◽  
Ehrlich Ascites ◽  
Trichosanthes Dioica

THE ROLE OF THIOL GROUPS IN THE DEVELOPMENT OF RADIATION DAMAGE IN THYMOCYTES AND EHRLICH ASCITES CARCINOMA CELLS

1964 ◽  
Vol 42 (12) ◽  
pp. 1717-1727 ◽  
Author(s):  
J. F. Scaife
Keyword(s):  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Irradiation Damage ◽  
Thiol Groups ◽  
Protein Thiol ◽  
Ehrlich Ascites ◽  
X Irradiation ◽  
The Difference

The effect of 800–1000 rads of X-irradiation on the thiol content of thymocytes and Ehrlich ascites carcinoma cells has been compared. Four hours after irradiation there was a decrease in the non-protein thiol (NP.SH) content of thymus and thymocytes but no change in ascites cells. In both cells the main NP.SH compound was glutathione. There was no significant effect of irradiation on the protein thiol (P.SH) content of thymus or ascites cells, but there was a slight decrease in P.SH in thymocytes after 4 hours incubation. Isolated thymus nuclei showed an immediate small decrease in P.SH content following 800 rads in vitro. Nuclei isolated from rat thymus 1 hour after 1000 rads in vivo showed an increase in the SH content of the globulin fraction and a decrease in the SH content of the nucleohistones. The total SH content of thymocytes and ascites cells was reduced by slow diffusion of H2O2into the cell suspension, but no effect of prior irradiation on this decrease of SH was found. Inhibition of catalase in vivo and in vitro did not produce any of the morphological signs of irradiation damage in thymocytes. There was no effect of irradiation on the copper content of thymus, thymocytes, or ascites cells. The ratio of NP.SH/P.SH is higher in thymocytes than in ascites cells, but, allowing for the difference in cell size, the overall total thiol concentration was the same. Anoxia produced only a small increase in NP.SH content in both cells and a small and doubtful increase in P.SH. It is concluded that, if thiol groups are involved in cell sensitivity to radiation, only a small fraction of the total SH groups are involved at critical sites.

Iron N-(2-hydroxy acetophenone) glycinate (FeNG), a non-toxic glutathione depletor circumvents doxorubicin resistance in Ehrlich ascites carcinoma cells in vivo

BioMetals ◽  
2011 ◽  
Vol 25 (1) ◽  
pp. 149-163 ◽  
Author(s):  
Avishek Ganguly ◽  
Paramita Chakraborty ◽  
Kaushik Banerjee ◽  
Shilpak Chatterjee ◽  
Soumya Basu ◽  
...  
Keyword(s):  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Doxorubicin Resistance ◽  
Ehrlich Ascites

Adaptation of ehrlich ascites carcinoma cells to energy deprivation in vivo can be associated with heat shock protein accumulation

1995 ◽  
Vol 165 (1) ◽  
pp. 1-6 ◽  
Author(s):  
A. E. Kabakov ◽  
A. O. Molotkov ◽  
K. R. Budagova ◽  
Yu M. Makarova ◽  
A. F. Mosin ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Protein Accumulation ◽  
Ehrlich Ascites ◽  
Energy Deprivation

ARGININE UPTAKE AND ARGINASE ACTIVITY IN EHRLICH ASCITES CARCINOMA CELLS

1959 ◽  
Vol 37 (4) ◽  
pp. 589-598 ◽  
Author(s):  
R. M. Johnstone
Keyword(s):  
Amino Acids ◽  
Tumor Cells ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Arginase Activity ◽  
Anaerobic Glycolysis ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Arginine Uptake ◽  
Ehrlich Ascites

Anaerobic glycolysis in Ehrlich ascites tumor cells exposed to amino acids leads to an increased uptake of a number of the amino acids to levels comparable with those obtained under aerobic conditions. The arginine uptake in the cell is not increased by glucose. Anaerobically the arginase activity is inhibited when glucose is present. The inhibition appears to be the result of an increased retention in the cell of the ornithine produced by arginase activity.

ARGININE UPTAKE AND ARGINASE ACTIVITY IN EHRLICH ASCITES CARCINOMA CELLS

1959 ◽  
Vol 37 (1) ◽  
pp. 589-598 ◽  
Author(s):  
R. M. Johnstone
Keyword(s):  
Amino Acids ◽  
Tumor Cells ◽  
Ehrlich Ascites Carcinoma ◽  
Carcinoma Cells ◽  
Arginase Activity ◽  
Anaerobic Glycolysis ◽  
Ehrlich Ascites Tumor Cells ◽  
Ascites Tumor ◽  
Arginine Uptake ◽  
Ehrlich Ascites

Anaerobic glycolysis in Ehrlich ascites tumor cells exposed to amino acids leads to an increased uptake of a number of the amino acids to levels comparable with those obtained under aerobic conditions. The arginine uptake in the cell is not increased by glucose. Anaerobically the arginase activity is inhibited when glucose is present. The inhibition appears to be the result of an increased retention in the cell of the ornithine produced by arginase activity.

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