scholarly journals Ultrasonographic, cosmetic, clinical and histological study of the healing process in the canine skin following surgical incision and closure using various techniques

2018 ◽  
Author(s):  
Δημήτριος Μπαλωμένος
Keyword(s):  
Histological Study ◽  
Healing Process ◽  
Surgical Incision ◽  
Poliglecaprone 25

Σκοπός της διατριβής ήταν η σύγκριση τεχνικών σύγκλισης τραύματος και η αξιολόγησή τους με τη βοήθεια κλινικής, υπερηχοτομογραφικής (50 MHz) και ιστοπαθολογικής εξέτασης, καθώς και η διερεύνηση της χρήσης της υπερηχοτομογραφίας στη διαγνωστική του δέρματος. Οι ακόλουθες τεχνικές χρησιμοποιήθηκαν για τη σύγκλιση τομών 12 εκατοστών στην εξωτερική επιφάνεια των μηρών 10 Beagle: (α) συρραπτικά δέρματος, (β) n-butyl cyanoacrylate κόλλα ιστών, γ. συνεχής ενδοδερμική ραφή με ενταφιασμό κόμπων (ράμμα 4-0 poliglecaprone-25) και (δ) συνεχής ενδοδερμική ραφή με καθήλωση με χρήση κουμπιών (ράμμα polypropylene 4-0). ΄Εγινε αισθητική, κλινική, υπερηχοτομογραφική και ιστολογική αξιολόγηση των τομών, σε τακτά χρονικά διαστήματα έως ένα έτος μετά τη σύγκλιση. Η αισθητική αξιολόγηση έγινε από δύο κριτές. Η κλινική αξιολόγηση περιελάμβανε την βαθμολόγηση του οιδήματος, ερυθήματος, εύρους ουλής, φλεγμονής ή αποστηματοποίησης, εξιδρώματος, κόμεδων, υπερχρωμίας, απώλειας συρραπτικών, διάσπασης τραύματος και σχηματισμού σταυροειδών ουλωδών εντυπωμάτων. Η υπερηχοτομογραφική αξιολόγηση περιελάμβανε μέτρηση της περιοχής επούλωσης σε τέσσερα διαφορετικά σημεία της τομής. Με την ιστολογική αξιολόγηση εκτιμήθηκε τυχόν νέκρωση, έλλειμμα επιδερμίδας, οίδημα, φλεγμονή, παρουσία υλικού συρραφής, ιστική αντίδραση πέριξ του υλικού συρραφής, πάχος επιδερμίδας, εύρος ουλής, παρουσία ινοβλαστών, παραγωγή κολλαγόνου και αγγειογένεση στην περιοχή επούλωσης. Τα συμπεράσματα της έρευνα είναι:Με όλες τις τεχνικές, η αισθητική αξιολόγηση βελτιωνόταν έως τον δεύτερο μήνα, μετά όμως η βελτίωση ήταν ελάχιστηΑπό την 9η μετεγχειρητική ημέρα και μετά, η κόλλα είχε τη χειρότερη αισθητική αξιολόγηση.Η ενδοδερμική ραφή με κουμπιά είχε την καλύτερη αισθητική εικόνα έως τον έκτο μήνα, στη συνέχεια όμως η ενδοδερμική με ενταφιασμό του κόμπου είχε καλύτερη εικόνα.Η ουλή είχε μεγαλύτερο εύρος με την κόλλα, η δε διαφορά ήταν στατιστικώς σημαντική σε σχέση με τις ενδοδερμικές στο τέλος του πειράματος.Η συνολική κλινική αξιολόγηση ήταν συνεχώς καλύτερη με τις ενδοδερμικές, με την ενδοδερμική με ενταφιασμό των κόμπων να υπερτερεί στο τέλος του πειράματος.Η υπερηχοτομογραφία δέρματος μπορεί να χρησιμοποιηθεί για την αξιολόγηση της επούλωσης δέρματος κατά πρώτο σκοπό, καθώς τα υπερηχοτομογραφικά ευρήματα αντικατόπτριζαν με ακρίβεια τα κλινικά και ιστολογικά ευρήματα.Τα ιστολογικά ευρήματα έδειξαν ότι η χρήση κόλλας προκαλεί έντονη πάχυνση της επιδερμίδας τον πρώτο μήνα της επούλωσης, ενώ, παρότι η εφαρμογή κόλλας έγινε με ιδιαίτερη προσοχή, μικρές ποσότητες κόλλας διείσδυσαν στο τραύμα, όπου παρέμειναν μέχρι το τέλος του πειράματος, προκαλώντας μικρές εστίες φλεγμονής.Η συνολική αξιολόγηση των τεχνικών έδειξε ότι οι ενδοδερμικές τεχνικές είχαν την καλύτερη εξέλιξη, με την ενδοδερμική ραφή με ενταφιασμό των κόμπων να υπερέχει, ενώ η χρήση κόλλας είχε την λιγότερο ικανοποιητική εικόνα. Η χρήση συρραπτικών είχε πολύ καλή εξέλιξη, ενώ ταυτόχρονα ήταν η γρηγορότερη και ευκολότερη τεχνική κατά την εφαρμογή, και μπορεί να εφαρμόζεται όταν υπάρχει πίεση χρόνου.

scholarly journals A Clinical and Histological Study about the Socket Preservation in a Patient under Oral Bisphosphonates Treatment: A Case Report

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 262
Author(s):  
Antonello Falco ◽  
Francesco Bataccia ◽  
Lorenzo Vittorini Orgeas ◽  
Federico Perfetti ◽  
Mariangela Basile ◽  
...  
Keyword(s):  
Case Report ◽  
Histological Study ◽  
Healing Process ◽  
Signs And Symptoms ◽  
Bisphosphonate Therapy ◽  
Graft Material ◽  
Oral Bisphosphonates ◽  
Socket Preservation ◽  
Oral Bisphosphonate ◽  
Histological Data

The aim of the present study is to assess the clinical and histological healing of a post-extractive alveolus following the procedure for socket preservation, in a patient receiving oral bisphosphonates for more than 6 years. After the extraction, enzymatically-deantigenated horse bone granules and an equine pericardium membrane were used to preserve the tooth socket. The patient was placed on a monthly follow-up in order to monitor the healing process. A 3 mm trephine bur was used to drill the bone for implant site preparation and to collect the bone sample. No signs and symptoms related to osteonecrosis of the jaws were reported. Histological data showed that, after 5 months, the mean percentages of trabecular bone, bone marrow and residual bone graft were respectively 45.74 ± 0.09%, 48.09 ± 0.08%, and 6.16 ± 0.01%. The residual graft material appeared to be osteointegrated and none of the particles appeared to be encapsulated. The present case report supports the guidelines that assume that patients undergoing oral bisphosphonate therapy can be eligible for surgical therapy. More clinical studies with larger sample sizes are needed to support this clinical evidence.

scholarly journals Histological study of tendon healing in malnourished Wistar rats treated with ultrasound therapy

2006 ◽  
Vol 21 (suppl 4) ◽  
pp. 13-17 ◽  
Author(s):  
Paulo de Tarso Camillo de Carvalho ◽  
Iandara Schettert Silva ◽  
Filipe Abdalla dos Reis ◽  
Ana Carulina Guimarães Belchior ◽  
Ricardo Dutra Aydos ◽  
...  
Keyword(s):  
Histological Study ◽  
Healing Process ◽  
Statistical Treatment ◽  
Tendon Healing ◽  
Tendon Injuries ◽  
Control Group ◽  
Test Results ◽  
Leukocyte Counts ◽  
Student’S T ◽  
Student’S T Test

PURPOSE: To investigate the effects of therapeutic ultrasound on the healing of tendon injuries in malnourished rats. METHODS: After the intended nutritional states had been attained, the animals (N=36) were distributed into three groups, named: G1(N=12), control group; G2(N=12), malnourished rats treated with 3 MHz ultrasound at an intensity of 0.5 W/cm²; and G3 (N=12), normal animals treated with 3 MHz at 0.5 W/cm². The injuries were induced by means of an operation to expose the heel tendon and crush it using Allis forceps. RESULTS: The data obtained relating to leukocyte counts, fibroblasts, vessel neoformation, fibrosis and collagen were subjected to statistical treatment using analysis of variance (ANOVA) and Student's t test. Results of p < 0.05 were obtained for fibrosis and collagen. CONCLUSION: The ultrasound was shown to be effective in repairing the tendon, even in malnourished animals whose healing process was retarded.

Negative-pressure wound therapy for high-risk surgical incision wounds using an ultra-portable device: 11 cases

2019 ◽  
Vol 7 (2) ◽  
pp. e000751
Author(s):  
Sofia Garcia-Pertierra ◽  
Nuria Vizcaino Reves ◽  
Araceli Calvo Aguado
Keyword(s):  
High Risk ◽  
Negative Pressure ◽  
Negative Pressure Wound Therapy ◽  
Healing Process ◽  
Case Series ◽  
Portable Device ◽  
Wound Therapy ◽  
Surgical Incision ◽  
Early Ambulation ◽  
Healing Wounds

Negative-pressure wound therapy (NPWT) involves the application of subatmospheric pressure to a wound to help in the healing process. The objective of this case series was to evaluate the clinical experience using ultra-portable NPWT devices on high-risk closed incisions after surgical management of complex non-healing wounds. Data were available for eight dogs and three cats. Wound aetiologies varied from traumatic, neoplastic and foreign body. Application and maintenance of the portable device were technically easy in most cases, finding some difficulties when applied to curved body surfaces. All patients’ outcome was highly rewarding. This case series suggests that portable NPWT helps in achieving full recovery, increases comfort allowing early ambulation and feeding, and reduces hospitalisation time. Their reduced size is suitable for smaller animals, enhancing their therapeutic use in veterinary medicine.

scholarly journals The Response of the Pulp-Dentine Complex, PDL, and Bone to Three Calcium Silicate-Based Cements: A Histological Study in an Animal Rat Model

2020 ◽  
Vol 2020 ◽  
pp. 1-13 ◽  
Author(s):  
Ranjdar Mahmood Talabani ◽  
Balkees Taha Garib ◽  
Reza Masaeli
Keyword(s):  
Inflammatory Response ◽  
Calcium Silicate ◽  
Histological Study ◽  
Healing Process ◽  
Mineral Trioxide Aggregate ◽  
Experimental Period ◽  
Tissue Reaction ◽  
Cortical Plate ◽  
Control Group ◽  
Albino Rats

Objective. The aim of this study was to histologically examine the tissue reaction of three different calcium silicate cements in the closure of perforations in rat incisor teeth. Material and Methods. An experimental lateral root perforation with pulp exposure was performed in 32 lower incisors of 16 male Wistar albino rats. They were randomly assigned into three test groups (each including eight teeth) that were filled either by Biodentine (BD) or MicroMega mineral trioxide aggregate (MM-MTA) or EndoSequence root repair material putty (ESRRM putty), besides eight unperforated incisors from the other four rats (control group). The inflammatory response and healing process were evaluated histologically and scored after one and four weeks. Differences among groups were tested by Kruskal–Wallis tests at P≤0.05. Results. In the first week, BD produced more inflammatory response in the pulpal (score 3) than other materials (score 2). Only ESRRM putty showed odontoblast-like cells in 50%, 25% dentine-like deposit, 25% evidence of bone deposition in the drilling site (score 2), and minimum periodontal ligament (PDL) necrosis and disorganization (25%, score 2). After one month, all groups had healthy pulpal tissue, but 25% of ESRRM putty retained score 1 inflammatory response, and 50% of the BD case had an incomplete palisading odontoblast layer (score 3). A thick and regular dentine bridge deposition was seen in the ESRRM putty group in comparison with MM-MTA and BD cases. The cortical plate healing in all ESRRM putty samples was complete (score 3), while an incomplete closure was seen in MM-MTA and BD groups (score 2). Both the MM-MTA and ESRRM putty groups had fully organized PDL (score 2), while in 50% of BD cases, a necrotizing area and disorganized PDL with inflammatory cells infiltration were still present. Statistically significant differences in the scores of any histologic parameters among the three tested materials were observed neither in the 1st nor in the 4th weeks of the experimental period. Conclusion. Better tissue compatibility and repair of pulpal and periodontal tissue have been detected after lateral perforation in the root of rat incisors when treated with ESRRM putty than MM-MTA and BD. However, the difference was not significant.

Histological Study of Resection Arthroplasty with and without Tendon Ball Interposition in Dogs

1999 ◽  
Vol 24 (3) ◽  
pp. 342-346 ◽  
Author(s):  
T. SARDENBERG ◽  
S. S. MÜLLER ◽  
H. R. PEREIRA ◽  
K. I. R. COELHO
Keyword(s):  
Wrist Joint ◽  
Histological Study ◽  
Healing Process ◽  
Repair Process ◽  
Resection Arthroplasty ◽  
Bone Resection ◽  
Group A ◽  
Group B ◽  
Extensor Carpi Radialis ◽  
Ischaemic Necrosis

We studied an experimental model of resection arthroplasty with or without tendon ball interposition in the wrist of dogs. Animals were divided into two groups. Animals in group A were treated by resection of the os carpi radiale with interposition of a ball made from the tendon of the extensor carpi radialis and the group B underwent bone resection alone. Animals were assessed 1, 6, 12 and 24 weeks after operation. In all of them the wrist joint was stable and had good mobility, allowing walking supported by the operated limb. In both groups biological material filled the cavity created by bone resection. A progressive repair process resulted in fibroplasia with areas of fibrocartilaginous metaplasia. The tendon ball showed complete ischaemic necrosis at the end of the first week, which delayed the healing process.

scholarly journals A Controlled Trial of Polyglytone 6211 versus Poliglecaprone 25 for Use in Intradermal Suturing in Dogs

Animals ◽  
2021 ◽  
Vol 11 (11) ◽  
pp. 3094
Author(s):  
Pagona Gouletsou ◽  
Nikitas Prassinos ◽  
Lysimachos Papazoglou ◽  
Polychronis Kostoulas ◽  
Apostolos Galatos
Keyword(s):  
Comparative Evaluation ◽  
Controlled Trial ◽  
Healing Process ◽  
Histological Evaluation ◽  
Beagle Dogs ◽  
Suture Materials ◽  
Experimental Animals ◽  
Clinical Appearance ◽  
Cosmetic Appearance ◽  
Poliglecaprone 25

The objective of this work was the comparative evaluation of the healing process after employing 4/0 poliglecaprone 25 and 4/0 polyglytone 6211 in a continuous intradermal suture pattern. Ten Beagle dogs were used, in which skin incisions were created surgically and subsequently were sutured by means of continuous intradermal pattern using polyglytone 6211 or poliglecaprone 25 suture. Cosmetic, clinical, and histologic scores were evaluated. The cosmetic appearance of the wounds was blindly evaluated on days 7, 14, 28, 180, 360, 730, and 1095. On the same days, tissue biopsy was performed for histological evaluation. Clinical evaluation was performed initially daily, then weekly, monthly, and finally yearly, till day 1095. The clinical appearance of the intradermal pattern with both sutures was initially very good, deteriorated in the second post-operative month and thereafter improved. The cosmetic, clinical, and histological differences between the two suture materials were minimal and statistically insignificant. Polyglytone 6211 is sufficient for use in intradermal suturing in dogs. However, its earlier absorption compared to poliglecaprone 25 did not have any beneficial effect on cutaneous wound healing and scar appearance in the experimental animals.

scholarly journals Effect of the magnetotherapy in the regeneration after tenotomy in animals magnetotherapy in the regeneration

2020 ◽  
pp. 1-8
Author(s):  
Adrian Guilhermo Carrillo Grimaldo ◽  
Rodrigo Marcel Valentim da Silva ◽  
Débora Antunes Pousa Bezerra ◽  
Luíza Batista Lima ◽  
Artur Dantas Freire ◽  
...  
Keyword(s):  
Electromagnetic Field ◽  
Achilles Tendon ◽  
Chronic Inflammation ◽  
Controlled Trial ◽  
Histological Study ◽  
Healing Process ◽  
Repair Process ◽  
Elastic Fibers ◽  
Control Group ◽  
Post Injury

Background: The Achilles tendon is the largest and most resistant tendon in the human body, being one of the most common areas of overload injury among athletes. The rupture occurs more frequently in football athletes, running and jumping, because these activities cause a great stress on the tendon during eccentric muscle contraction of the sural triceps. Among the different physiotherapy resources used to repair tendon injuries, there is magnetotherapy, which is a feature that uses a pulsatile electromagnetic field, a suggested inducer of the acceleration of regeneration. Objective: To investigate the effects of magnetotherapy on the organization of collagen and elastic fibers and inflammatory process induced in different days and phases of the healing process of Achilles tendon injury in rats. Method: This study is a randomized controlled trial. Thirty female Wistar rats were randomly divided into 5 groups (each with n = 6), submitted to tenotomy by transverse shear in the middle third of the right Achilles tendon, except for group 1, which was control group without injury, sacrificed on day 14. Group 2, control with injury, sacrificed on day 7; group 3 was treated and received 5 pulsed electromagnetic field (PEMF) applications from 24h post injury and sacrificed on day 7; group 4, control with injury and sacrificed on day 14; and group 5 received 12 PEMF applications from 24h post injury and sacrificed on day 14. The parameters used were 20 mT, 50Hz and 30 minutes of application in each session, using the Magnetherp 330 digital device (Meditea). At the end of treatment, a histological study was performed to evaluate the amount of collagen and elastic cells and fibers and to compare the degree of organization of collagen fibers. Results: G - 3, compared to its control G - 2 showed lower acute inflammatory response; G - 5 did not show acute and chronic inflammation and possessed an accelerated process of repair without fibrosis, unlike its control. Conclusion: The magnetotherapy decreased the signs of inflammation in the acute phase, accelerated the repair process with the onset of fibrosis in 7 days; and at 14 days the tendon tissue was healed, with organizing fibrosis, without acute or chronic inflammation.

scholarly journals Perioperative hypothermia and incidence of surgical wound infection: a bibliographic study

2014 ◽  
Vol 12 (4) ◽  
pp. 513-517 ◽  
Author(s):  
Aline Batista da Silva ◽  
Aparecida de Cassia Giani Peniche
Keyword(s):  
Selection Criteria ◽  
Healing Process ◽  
Perioperative Period ◽  
Review Article ◽  
Surgical Wound Infection ◽  
Health Science ◽  
Surgical Wound ◽  
Surgical Incision ◽  
Perioperative Hypothermia ◽  
Bibliographic Study

The purpose of this review article was to understand and analyze the scientific production related to the occurrence of perioperative hypothermia and the incidence of infection on the surgical site. For this purpose, a search was conducted in the databases LILACS, MEDLINE, PubMed, CINAHL and Cochrane, using the health science descriptors DECS, from 2004 to 2009. A total of 91 articles were found. After eliminating duplicate items and using selection criteria for inclusion, six manuscripts remained for analysis. The studies were classified as retrospective, prospective, case studies, and clinical trials. After analysis, the majority of studies showed that hypothermia must be prevented during the perioperative period to reduce complications in the healing process of the surgical incision. Therefore, unadverted hypothermia directly influences in surgical site healing, increasing the incidence of infection in the surgical wound.

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