scholarly journals Ανίχνευση C-αντιδρώσας πρωτεΐνης και D-διμερών σε μικροσυστοιχίες με φασματοσκοπία ανάκλασης λευκού φωτός

2015 ◽  
Author(s):  
Γεώργιος Κουκουβίνος
Keyword(s):  
Simultaneous Detection ◽  
Modern World ◽  
Cardiac Markers ◽  
Optimal Conditions ◽  
C Reactive Protein ◽  
Transparent Material ◽  
Reactive Protein ◽  
Time Flow ◽  
Optical Immunosensor

Cardiovascular diseases are one of the main death causes in the modern world. Forthis reason, the determination of the concentration in the serum of particular proteins,called cardiac markers, is vital both for determining the extent of the damage after anischemic incident as well as for predicting a cardiac disease. The aim of this workwas to develop a method to simultaneously detect two markers that can help inpredicting a heart attack, namely C-reactive protein (CRP) and D-Dimers usingantibodies microarrays in combination with an optical immunosensor based on whitelight reflectance spectroscopy and allows the detection of biomolecular reactions inreal time without the use of labels. In particular, detection is based on monitoringchanges in interference spectrum created upon reflection of white light on a siliconsurface covered with a suitable film of transparent material on which the analytespecificantibodies are immobilized. At first, suitable antibodies were selected throughthe development of non-competitive enzyme immunoassays for the two analytes inmicrotitration wells. After that, the immunochemical assays were transferred to theoptical sensor, wherein the thickness and composition of the transparent filmdeposited on the silicon surface as well as the method for the chemical modificationof the surface and immobilization of antibodies were optimized. Subsequently, all theparameters of immunochemical determinations with the sensor were optimizedincluding antibody concentrations, immunoreaction time, flow rate, etc. By applying the optimal conditions, calibration curves for both markers were obtained and therepeatability and reliability of the measurements performed with the developedimmunosensor were evaluated. Finally, discrete zones of the two specific antibodiesagainst CRP and D-Dimers were created by spotting them onto the same surfaceand the surfaces were used for the simultaneous detection of the two analytes inserum samples. The values determined for both analytes were in good agreementwith those determined for the same samples with reference methods, demonstratingthe reliability of the determinations performed with the developed immunosensor andits potential for application in the simultaneous determination of two markers inclinical samples.

C-REACTIVE PROTEIN IN THE SERUM IN NEPHROSIS AND ACUTE GLOMERULONEPHRITIS

PEDIATRICS ◽  
1960 ◽  
Vol 25 (1) ◽  
pp. 106-111
Author(s):  
Aree Valyasevi ◽  
Joseph M. Sloan ◽  
Lewis A. Barness
Keyword(s):  
Nephrotic Syndrome ◽  
Hormone Therapy ◽  
Erythrocyte Sedimentation Rate ◽  
Sedimentation Rate ◽  
Positive Test ◽  
Acute Glomerulonephritis ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Erythrocyte Sedimentation

C-reactive protein in the serum and the erythrocyte sedimentation rate were followed serially in 13 patients with acute nephritis and in 9 patients with the nephrotic syndrome. Although a positive C-reactive protein test was always associated with evidence of infection, obvious infection in a few instances was not accompanied by a positive test. Determination of C-reactive protein is valuable in determining the presence of infection in patients with acute nephritis and the nephrotic syndrome, especially in the latter where hormone therapy may mask many signs of infection.

Comparison of Three Commercial Assays for the Determination of Serum C-Reactive Protein

1999 ◽  
Vol 7 (2) ◽  
pp. 45-59
Author(s):  
TARIF ZAWAWI ◽  
IBRAHTM HASHIM ◽  
WALEED AL-YAFI ◽  
MOHAMMED ABDELLAAL
Keyword(s):  
C Reactive Protein ◽  
Reactive Protein

scholarly journals Stimulation of Erythrocyte Cell Membrane Scrambling by C-Reactive Protein

2017 ◽  
Vol 41 (2) ◽  
pp. 806-818 ◽  
Author(s):  
Majed Abed ◽  
Christian Thiel ◽  
Syeda T. Towhid ◽  
Kousi Alzoubi ◽  
Sabina Honisch ◽  
...  
Keyword(s):  
Plasma Concentration ◽  
Healthy Volunteers ◽  
Annexin V ◽  
Caspase Activation ◽  
C Reactive Protein ◽  
Forward Scatter ◽  
Reactive Protein ◽  
Phosphatidylserine Translocation ◽  
Binding Cell

Background: Eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine-translocation, is triggered by fever and inflammation. Signaling includes increased cytosolic Ca2+-activity ([Ca2+]i), caspase activation, and ceramide. Inflammation is associated with increased plasma concentration of C-reactive protein (CRP). The present study explored whether CRP triggers eryptosis. Methods: Phosphatidylserine abundance at the cell surface was estimated from annexin-V-binding, cell volume from forward scatter, [Ca2+]i from Fluo3-fluorescence, ceramide abundance and caspase-3-activity utilizing FITC-conjugated antibodies. Moreover, blood was drawn from patients with acute appendicitis (9♀,11♂) and healthy volunteers (10♀,10♂) for determination of CRP, blood count and phosphatidylserine. Results: A 48h CRP treatment significantly increased the percentage of annexin-V-binding cells (≥5µg/ml), [Ca2+]i (≥5µg/ml), ceramide (20µg/ml) and caspase-activity (20µg/ml). Annexin-V-binding was significantly blunted by caspase inhibitor zVAD (10µM). The percentage of phosphatidylserine-exposing erythrocytes in freshly drawn blood was significantly higher in appendicitis patients (1.83±0.21%) than healthy volunteers (0.81±0.09%), and significantly higher following a 24h incubation of erythrocytes from healthy volunteers to patient plasma than to plasma from healthy volunteers. The percentage of phosphatidylserine-exposing erythrocytes correlated with CRP plasma concentration. Conclusion: C-reactive protein triggers eryptosis, an effect at least partially due to increase of [Ca2+]i, increase of ceramide abundance and caspase activation.

scholarly journals Dynamics of inflammatory factors expression in ischemic brain tissue injury

2019 ◽  
Vol 11 (4) ◽  
Author(s):  
Tonko Marinović ◽  
Silvio Bašić ◽  
Dominik Romić ◽  
Branimir Nevajda ◽  
Lovorka Đerek ◽  
...  
Keyword(s):  
Ischemic Stroke ◽  
Tissue Injury ◽  
Interleukin 10 ◽  
Inflammatory Factors ◽  
Modern World ◽  
C Reactive Protein ◽  
High Association ◽  
Reactive Protein ◽  
Initial Onset

Ischemic stroke is one of the most common cause of mortality and disability in the modern world. Still, therapeutic options remains modest. Aim of the study was to present dynamics of inflammatory factors expression (C reactive protein, procalcitonin, interleukin 10) in patients after ischemic stroke. Our study included 101 patients divided in thrombolised and non-thrombolised groups. Inflammatory factors concentration in serum was determinate at admission, 24, 48 hours and seven days after the initial onset, while neurological assessment was measured at the admission, 24 hours, seven days and three months after the initial onset using National Institute of Health Stroke Scale and Rankin Scale. Certain pattern was observed in dynamics of inflammatory factors: intensive increase in first and second day after the stroke, followed by decrease till day seven in both groups. Additionally, thrombolised group showed significant neurological improvement. Although well investigated, the role of inflammatory factors in the ischemic stroke still stays controversial. High association of C reactive protein and interleukin 10 values suggest potential prognostic role in patients follow-up, while the role of procalcitonin values still remains unclear.

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