scholarly journals A Novel Fluorescent Biosensor for Ultrasensitive Detection of miRNA-122 Based on Rolling Circle Amplification Coupled with Nicking Enzyme Signal Amplification

2017 ◽  
Vol 05 (04) ◽  
pp. 53-62
Author(s):  
煦 王
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle ◽  
Fluorescent Biosensor ◽  
Nicking Enzyme

A fluorescent biosensor for highly specific and ultrasensitive detection of adenosine triphosphate based on ligation-triggered branched rolling circle amplification

2019 ◽  
Vol 11 (36) ◽  
pp. 4629-4636
Author(s):  
Hongxin Jiang ◽  
Guimei Han ◽  
Yaping Xu ◽  
Junxing Li ◽  
Xiaowei Liu ◽  
...  
Keyword(s):  
Adenosine Triphosphate ◽  
Rolling Circle Amplification ◽  
Dna Ligase ◽  
Ultrasensitive Detection ◽  
Selective Detection ◽  
Rolling Circle ◽  
T4 Dna Ligase ◽  
Fluorescent Sensing ◽  
Fluorescent Biosensor

A fluorescent sensing strategy for ultrasensitive and highly selective detection of ATP was presented by taking advantage of the exponential amplification capability of BRCA and the extreme specificity of T4 DNA ligase toward ATP.

Base excision repair initiated rolling circle amplification-based fluorescent assay for screening uracil-DNA glycosylase activity using Endo IV-assisted cleavage of AP probes

The Analyst ◽  
2018 ◽  
Vol 143 (16) ◽  
pp. 3951-3958 ◽  
Author(s):  
Jingfeng Wang ◽  
Yu Wang ◽  
Su Liu ◽  
Haiwang Wang ◽  
Xue Zhang ◽  
...  
Keyword(s):  
Base Excision Repair ◽  
Signal Amplification ◽  
Excision Repair ◽  
Rolling Circle Amplification ◽  
Cost Effective ◽  
Ultrasensitive Detection ◽  
Fluorescent Assay ◽  
Uracil Dna Glycosylase ◽  
Rolling Circle ◽  
Base Excision

A simple, robust and cost effective biosensing platform for the ultrasensitive detection of UDG activity was established based on base excision repair-initiated primer generation for RCA with Endo IV-assisted signal amplification.

An aptamer-based chemiluminescence method for ultrasensitive detection of platelet-derived growth factor by cascade amplification combining rolling circle amplification with hydroxylamine-enlarged gold nanoparticles

2015 ◽  
Vol 7 (20) ◽  
pp. 8786-8792 ◽  
Author(s):  
Lu-Yan Yao ◽  
Xiao-Qian Yu ◽  
Yan-Jun Zhao ◽  
Ai-Ping Fan
Keyword(s):  
Gold Nanoparticles ◽  
Growth Factor ◽  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Platelet Derived Growth Factor ◽  
Ultrasensitive Detection ◽  
Chemiluminescence Method ◽  
Rolling Circle ◽  
Chemiluminescence Assay ◽  
Cascade Amplification

An ultrasensitive and selective chemiluminescence assay for PDGF-BB detection using the powerful signal amplification capability of RCA and hydroxylamine-amplified AuNPs.

scholarly journals A dual-signal amplification platform for sensitive fluorescence biosensing of leukemia-derived exosomes

Nanoscale ◽  
2018 ◽  
Vol 10 (43) ◽  
pp. 20289-20295 ◽  
Author(s):  
Lin Huang ◽  
Dian-Bing Wang ◽  
Netrapal Singh ◽  
Fang Yang ◽  
Ning Gu ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Rolling Circle ◽  
Nicking Endonuclease ◽  
Fluorescent Biosensor ◽  
Dual Signal Amplification

We report a rolling circle amplification and nicking endonuclease-assisted dual signal amplification fluorescent biosensor for exosome detection.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

Ultrasensitive detection of lung cancer-associated miRNAs by multiple primer-mediated rolling circle amplification coupled with a graphene oxide fluorescence-based (MPRCA-GO) sensor

The Analyst ◽  
2019 ◽  
Vol 144 (14) ◽  
pp. 4180-4187 ◽  
Author(s):  
Krissana Khoothiam ◽  
Kiatnida Treerattrakoon ◽  
Tawin Iempridee ◽  
Patraporn Luksirikul ◽  
Tararaj Dharakul ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Graphene Oxide ◽  
Cancer Diagnosis ◽  
Rolling Circle Amplification ◽  
Ultrasensitive Detection ◽  
Rolling Circle

MPRCA-GO sensor can detect low amount of miRNAs, which are biomarkers in cancer diagnosis.

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