An aptamer-based chemiluminescence method for ultrasensitive detection of platelet-derived growth factor by cascade amplification combining rolling circle amplification with hydroxylamine-enlarged gold nanoparticles

2015 ◽  
Vol 7 (20) ◽  
pp. 8786-8792 ◽  
Author(s):  
Lu-Yan Yao ◽  
Xiao-Qian Yu ◽  
Yan-Jun Zhao ◽  
Ai-Ping Fan
Keyword(s):  
Gold Nanoparticles ◽  
Growth Factor ◽  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Platelet Derived Growth Factor ◽  
Ultrasensitive Detection ◽  
Chemiluminescence Method ◽  
Rolling Circle ◽  
Chemiluminescence Assay ◽  
Cascade Amplification

An ultrasensitive and selective chemiluminescence assay for PDGF-BB detection using the powerful signal amplification capability of RCA and hydroxylamine-amplified AuNPs.

A cascade amplification strategy based on rolling circle amplification and hydroxylamine amplified gold nanoparticles enables chemiluminescence detection of adenosine triphosphate

The Analyst ◽  
2014 ◽  
Vol 139 (15) ◽  
pp. 3796-3803 ◽  
Author(s):  
Ping Wang ◽  
Tonghuan Zhang ◽  
Taoyi Yang ◽  
Nan Jin ◽  
Yanjun Zhao ◽  
...  
Keyword(s):  
Gold Nanoparticles ◽  
Adenosine Triphosphate ◽  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Enzymatic Reaction ◽  
Chemiluminescence Detection ◽  
Rolling Circle ◽  
Amplification Strategy ◽  
Cascade Amplification

A chemiluminescent biosensor for ATP was developed by taking advantage of the ATP-dependent enzymatic reaction, the powerful signal amplification capability of rolling circle amplification, and hydroxylamine-amplified gold nanoparticles.

scholarly journals Aptamer and rolling circle amplification-involved sandwich assay for platelet-derived growth factor-BB with absorbance analysis

2015 ◽  
Vol 7 (5) ◽  
pp. 1855-1859 ◽  
Author(s):  
Li Lv ◽  
Limin Guo ◽  
Qiang Zhao
Keyword(s):  
Growth Factor ◽  
Horseradish Peroxidase ◽  
Rolling Circle Amplification ◽  
Platelet Derived Growth Factor ◽  
Sandwich Assay ◽  
Rolling Circle ◽  
Amplification Reaction

An aptamer-based absorbance assay for PDGF-BB was developed by combining rolling circle amplification reaction and catalysis with horseradish peroxidase.

Base excision repair initiated rolling circle amplification-based fluorescent assay for screening uracil-DNA glycosylase activity using Endo IV-assisted cleavage of AP probes

The Analyst ◽  
2018 ◽  
Vol 143 (16) ◽  
pp. 3951-3958 ◽  
Author(s):  
Jingfeng Wang ◽  
Yu Wang ◽  
Su Liu ◽  
Haiwang Wang ◽  
Xue Zhang ◽  
...  
Keyword(s):  
Base Excision Repair ◽  
Signal Amplification ◽  
Excision Repair ◽  
Rolling Circle Amplification ◽  
Cost Effective ◽  
Ultrasensitive Detection ◽  
Fluorescent Assay ◽  
Uracil Dna Glycosylase ◽  
Rolling Circle ◽  
Base Excision

A simple, robust and cost effective biosensing platform for the ultrasensitive detection of UDG activity was established based on base excision repair-initiated primer generation for RCA with Endo IV-assisted signal amplification.

scholarly journals MiRNA Detection Using a Rolling Circle Amplification and RNA-Cutting Allosteric Deoxyribozyme Dual Signal Amplification Strategy

Biosensors ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 222
Author(s):  
Chenxin Fang ◽  
Ping Ouyang ◽  
Yuxing Yang ◽  
Yang Qing ◽  
Jialun Han ◽  
...  
Keyword(s):  
Signal Amplification ◽  
Rolling Circle Amplification ◽  
Padlock Probe ◽  
Rolling Circle ◽  
Sensing Platform ◽  
Mirna Detection ◽  
Substrate Cleavage ◽  
Amplification Strategy ◽  
Circular Template ◽  
Dual Signal Amplification

A microRNA (miRNA) detection platform composed of a rolling circle amplification (RCA) system and an allosteric deoxyribozyme system is proposed, which can detect miRNA-21 rapidly and efficiently. Padlock probe hybridization with the target miRNA is achieved through complementary base pairing and the padlock probe forms a closed circular template under the action of ligase; this circular template results in RCA. In the presence of DNA polymerase, RCA proceeds and a long chain with numerous repeating units is formed. In the presence of single-stranded DNA (H1 and H2), multi-component nucleic acid enzymes (MNAzymes) are formed that have the ability to cleave substrates. Finally, substrates containing fluorescent and quenching groups and magnesium ions are added to the system to activate the MNAzyme and the substrate cleavage reaction, thus achieving fluorescence intensity amplification. The RCA–MNAzyme system has dual signal amplification and presents a sensing platform that demonstrates broad prospects in the analysis and detection of nucleic acids.

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