HISTOCHEMICAL STUDY ON EFFECT OF FREEZING AND THAWING ON TETRAZOLIUM REDUCIBILITY OF THE HAIR CELLS OF THE INNER EAR IN GUINEA PIGS

The last decade has witnessed the identification of several families affected by hereditary non-syndromic hearing loss (NSHL) caused by mutations in the SMPX gene and the loss of function has been suggested as the underlying mechanism. In the attempt to confirm this hypothesis we generated an Smpx-deficient zebrafish model, pointing out its crucial role in proper inner ear development. Indeed, a marked decrease in the number of kinocilia together with structural alterations of the stereocilia and the kinocilium itself in the hair cells of the inner ear were observed. We also report the impairment of the mechanotransduction by the hair cells, making SMPX a potential key player in the construction of the machinery necessary for sound detection. This wealth of evidence provides the first possible explanation for hearing loss in SMPX-mutated patients. Additionally, we observed a clear muscular phenotype consisting of the defective organization and functioning of muscle fibers, strongly suggesting a potential role for the protein in the development of muscle fibers. This piece of evidence highlights the need for more in-depth analyses in search for possible correlations between SMPX mutations and muscular disorders in humans, thus potentially turning this non-syndromic hearing loss-associated gene into the genetic cause of dysfunctions characterized by more than one symptom, making SMPX a novel syndromic gene.

Download Full-text

Identification and characterization of amphibian SLC26A5 using RNA-Seq

BMC Genomics ◽

10.1186/s12864-021-07798-6 ◽

2021 ◽

Vol 22 (1) ◽

Author(s):

Zhongying Wang ◽

Qixuan Wang ◽

Hao Wu ◽

Zhiwu Huang

Keyword(s):

Amino Acid ◽

Inner Ear ◽

Hair Cells ◽

Rana Catesbeiana ◽

Site Directed Mutagenesis ◽

Amino Acid Residues ◽

Rna Seq ◽

American Bullfrog ◽

Frog Species

Abstract Background Prestin (SLC26A5) is responsible for acute sensitivity and frequency selectivity in the vertebrate auditory system. Limited knowledge of prestin is from experiments using site-directed mutagenesis or domain-swapping techniques after the amino acid residues were identified by comparing the sequence of prestin to those of its paralogs and orthologs. Frog prestin is the only representative in amphibian lineage and the studies of it were quite rare with only one species identified. Results Here we report a new coding sequence of SLC26A5 for a frog species, Rana catesbeiana (the American bullfrog). In our study, the SLC26A5 gene of Rana has been mapped, sequenced and cloned successively using RNA-Seq. We measured the nonlinear capacitance (NLC) of prestin both in the hair cells of Rana’s inner ear and HEK293T cells transfected with this new coding gene. HEK293T cells expressing Rana prestin showed electrophysiological features similar to that of hair cells from its inner ear. Comparative studies of zebrafish, chick, Rana and an ancient frog species showed that chick and zebrafish prestin lacked NLC. Ancient frog’s prestin was functionally different from Rana. Conclusions We mapped and sequenced the SLC26A5 of the Rana catesbeiana from its inner ear cDNA using RNA-Seq. The Rana SLC26A5 cDNA was 2292 bp long, encoding a polypeptide of 763 amino acid residues, with 40% identity to mammals. This new coding gene could encode a functionally active protein conferring NLC to both frog HCs and the mammalian cell line. While comparing to its orthologs, the amphibian prestin has been evolutionarily changing its function and becomes more advanced than avian and teleost prestin.

Download Full-text

Hes1andHes5Activities Are Required for the Normal Development of the Hair Cells in the Mammalian Inner Ear

Journal of Neuroscience ◽

10.1523/jneurosci.21-13-04712.2001 ◽

2001 ◽

Vol 21 (13) ◽

pp. 4712-4720 ◽

Cited By ~ 196

Author(s):

Azel Zine ◽

Alexandre Aubert ◽

Jiping Qiu ◽

Stavros Therianos ◽

Francois Guillemot ◽

...

Keyword(s):

Inner Ear ◽

Hair Cells ◽

Normal Development

Download Full-text

Immunology of the inner ear: Response of the inner ear to antigen challenge

Otolaryngology ◽

10.1177/019459988309100105 ◽

1983 ◽

Vol 91 (1) ◽

pp. 18-23 ◽

Cited By ~ 106

Author(s):

Jeffrey P. Harris

Keyword(s):

Inner Ear ◽

Blood Brain Barrier ◽

Guinea Pigs ◽

Keyhole Limpet Hemocyanin ◽

Host Immunity ◽

Brain Barrier ◽

Antigen Challenge ◽

Antibody Titers ◽

Relationship Of ◽

The Relationship

The relationship of the inner ear to host immunity and the immunoresponsiveness of the inner ear to antigen challenge were investigated. A radioimmunoassay was used to quantitate antibody titers to keyhole-limpet hemocyanin generated in the serum, perilymph, and CSF of guinea pigs following systemic or inner ear immunizations. The results of these experiments demonstrate (1) the blood-labyrinth barrier is analogous to the blood-brain barrier with respect to immunoglobulin equilibrium, (2) the inner ear is capable of responding to antigen challenge, and (3) the inner ear is an effective route for systemic immunization.

Download Full-text

Nonlinearities in Threshold-Level Detection by Inner Ear Hair Cells

Biophysical Journal ◽

10.1016/j.bpj.2011.11.3568 ◽

2012 ◽

Vol 102 (3) ◽

pp. 655a

Author(s):

Yuttana Roongthumskul ◽

Albert Kao ◽

Sebastiaan W.F. Meenderink ◽

Dolores Bozovic

Keyword(s):

Inner Ear ◽

Hair Cells ◽

Threshold Level

Download Full-text

Hes1 is a negative regulator of inner ear hair cell differentiation

Development ◽

10.1242/dev.127.21.4551 ◽

2000 ◽

Vol 127 (21) ◽

pp. 4551-4560 ◽

Cited By ~ 5

Author(s):

J.L. Zheng ◽

J. Shou ◽

F. Guillemot ◽

R. Kageyama ◽

W.Q. Gao

Keyword(s):

Cell Differentiation ◽

Hair Cell ◽

Inner Ear ◽

Cell Fate ◽

Hair Cells ◽

Outer Hair Cells ◽

Negative Regulator ◽

Pcr Analysis ◽

Loss Of Function ◽

Hair Cell Differentiation

Hair cell fate determination in the inner ear has been shown to be controlled by specific genes. Recent loss-of-function and gain-of-function experiments have demonstrated that Math1, a mouse homolog of the Drosophila gene atonal, is essential for the production of hair cells. To identify genes that may interact with Math1 and inhibit hair cell differentiation, we have focused on Hes1, a mammalian hairy and enhancer of split homolog, which is a negative regulator of neurogenesis. We report here that targeted deletion of Hes1 leads to formation of supernumerary hair cells in the cochlea and utricle of the inner ear. RT-PCR analysis shows that Hes1 is expressed in inner ear during hair cell differentiation and its expression is maintained in adulthood. In situ hybridization with late embryonic inner ear tissue reveals that Hes1 is expressed in supporting cells, but not hair cells, of the vestibular sensory epithelium. In the cochlea, Hes1 is selectively expressed in the greater epithelial ridge and lesser epithelial ridge regions which are adjacent to inner and outer hair cells. Co-transfection experiments in postnatal rat explant cultures show that overexpression of Hes1 prevents hair cell differentiation induced by Math1. Therefore Hes1 can negatively regulate hair cell differentiation by antagonizing Math1. These results suggest that a balance between Math1 and negative regulators such as Hes1 is crucial for the production of an appropriate number of inner ear hair cells.

Download Full-text