scholarly journals HISTOCHEMICAL DEMONSTRATION OF FRUCTOSE 1, 6-DIPHOSPHATASE IN RAT LIVER USING NITRO-BLUE TETRAZOLIUM

1974 ◽  
Vol 7 (3) ◽  
pp. 184-190 ◽  
Author(s):  
HAJIME AOE
Keyword(s):  
Rat Liver ◽  
Histochemical Demonstration ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium

scholarly journals Interactions of some acceptors with superoxide anion radicals formed by the NADPH-specific flavoprotein in rat liver microsomal fractions

1976 ◽  
Vol 154 (2) ◽  
pp. 307-310 ◽  
Author(s):  
V Mishin ◽  
A Pokrovsky ◽  
V V Lyakhovich
Keyword(s):  
Rat Liver ◽  
Superoxide Anion ◽  
Microsomal Fraction ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Liver Microsomal Fraction ◽  
Dependent Pathway ◽  
Tetrazolium Reduction ◽  
Superoxide Anion Radicals ◽  
Anion Radicals

In rat liver microsomal fractions oxidation of adrenaline was effected by superoxide anion radicals (O2-), whereas cytochrome c, 2,6-dichlorophenol-indophenol and ferricyanide accepted electrons from NADPH-specific flavoprotein only directly. Nitro Blue Tetrazolium was reduced both by O2- and by the direct acceptance of electrons. Elevation of pH and addition of menadione shift the Nitro Blue Tetrazolium reduction towards the O2--dependent pathway. From the values of the kinetic constants for interaction of adrenaline and Nitro Blue Tetrazolium with NADPH-specific flavoprotein, the rates of generation of O2- in rat liver microsomal fraction were determined.

Fructosamine Test-Plus, a modified fructosamine assay evaluated

1991 ◽  
Vol 37 (4) ◽  
pp. 552-556 ◽  
Author(s):  
John Baker ◽  
Patricia Metcalf ◽  
Robert Scragg ◽  
Roger Johnson
Keyword(s):  
Diabetes Mellitus ◽  
Protein Concentration ◽  
Screening Method ◽  
Health Screening ◽  
Disease Prevalence ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Screening Survey

Abstract We have evaluated Fructosamine Test-Plus, a commercial fructosamine assay based on the reduction of nitro-blue tetrazolium dye in alkaline buffer (Clin Chem 1985;31:1550-4), modified by including a detergent and uricase in the reagent, by changing the concentrations of buffer and dye, and by changing the approach to primary calibration. Specimens were from 2321 participants in a health screening survey in a local workforce. Compared with the original fructosamine method, the Fructosamine Test-Plus method was less affected by protein concentration in the sample and less subject to interference from hyperlipidemia. The changes have also extended the linearity of the assay in the pathological range. However, as a screening method for diabetes mellitus in a population with a disease prevalence of 2.28%, the performance of Fructosamine Test-Plus was similar to the original assay.

ИЗМЕНЕНИЯ ИММУНОЛОГИЧЕСКОГО ОТВЕТА В РЕАКЦИИ НСТ С ПЕРИТОНЕАЛЬНЫМИ МАКРОФАГАМИ ПОД ДЕЙСТВИЕМ ШИРОКОПОЛОСНОГО ЭЛЕКТРОМАГНИТНОГО ИЗЛУЧЕНИЯ В ЭКСПЕРИМЕНТЕ

Innova ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 55-57
Author(s):  
Андрей Сергеевич Руцкой ◽  
◽  
Вячеслав Александрович Липатов ◽  
Александр Анатольевич Панов ◽  
Илья Игоревич Шляпцев ◽  
...  
Keyword(s):  
Immune System ◽  
Enzymatic Activity ◽  
Electromagnetic Fields ◽  
Peritoneal Macrophages ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Living Organisms ◽  
Different Characteristics

The article presents the results of research on the study of the effect of broadband electromagnetic fields on a macroorganism in an experiment. Electromagnetic fields with different characteristics can affect the systems of living organisms in different ways, including the immune system, which is sensitive to electromagnetic influences. Broadband electromagnetic fields have an immunostimulating effect, as evidenced by the increase in the enzymatic activity of peritoneal macrophages in reaction with nitro blue tetrazolium.

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