scholarly journals OBSERVATIONS ON THE SUBCELLULAR LOCALIZATION OF OXIDATIVE ENZYMES WITH NITRO BLUE TETRAZOLIUM

1963 ◽  
Vol 11 (1) ◽  
pp. 62-70 ◽  
Author(s):  
JEAN WALTER HITZEMAN
Keyword(s):  
Subcellular Localization ◽  
Nitro Blue Tetrazolium ◽  
Oxidative Enzymes ◽  
Blue Tetrazolium

Fructosamine Test-Plus, a modified fructosamine assay evaluated

1991 ◽  
Vol 37 (4) ◽  
pp. 552-556 ◽  
Author(s):  
John Baker ◽  
Patricia Metcalf ◽  
Robert Scragg ◽  
Roger Johnson
Keyword(s):  
Diabetes Mellitus ◽  
Protein Concentration ◽  
Screening Method ◽  
Health Screening ◽  
Disease Prevalence ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Screening Survey

Abstract We have evaluated Fructosamine Test-Plus, a commercial fructosamine assay based on the reduction of nitro-blue tetrazolium dye in alkaline buffer (Clin Chem 1985;31:1550-4), modified by including a detergent and uricase in the reagent, by changing the concentrations of buffer and dye, and by changing the approach to primary calibration. Specimens were from 2321 participants in a health screening survey in a local workforce. Compared with the original fructosamine method, the Fructosamine Test-Plus method was less affected by protein concentration in the sample and less subject to interference from hyperlipidemia. The changes have also extended the linearity of the assay in the pathological range. However, as a screening method for diabetes mellitus in a population with a disease prevalence of 2.28%, the performance of Fructosamine Test-Plus was similar to the original assay.

ИЗМЕНЕНИЯ ИММУНОЛОГИЧЕСКОГО ОТВЕТА В РЕАКЦИИ НСТ С ПЕРИТОНЕАЛЬНЫМИ МАКРОФАГАМИ ПОД ДЕЙСТВИЕМ ШИРОКОПОЛОСНОГО ЭЛЕКТРОМАГНИТНОГО ИЗЛУЧЕНИЯ В ЭКСПЕРИМЕНТЕ

Innova ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 55-57
Author(s):  
Андрей Сергеевич Руцкой ◽  
◽  
Вячеслав Александрович Липатов ◽  
Александр Анатольевич Панов ◽  
Илья Игоревич Шляпцев ◽  
...  
Keyword(s):  
Immune System ◽  
Enzymatic Activity ◽  
Electromagnetic Fields ◽  
Peritoneal Macrophages ◽  
Nitro Blue Tetrazolium ◽  
Blue Tetrazolium ◽  
Living Organisms ◽  
Different Characteristics

The article presents the results of research on the study of the effect of broadband electromagnetic fields on a macroorganism in an experiment. Electromagnetic fields with different characteristics can affect the systems of living organisms in different ways, including the immune system, which is sensitive to electromagnetic influences. Broadband electromagnetic fields have an immunostimulating effect, as evidenced by the increase in the enzymatic activity of peritoneal macrophages in reaction with nitro blue tetrazolium.

Radiation-induced coloration of nitro blue tetrazolium gel dosimeter for low dose applications

2017 ◽  
Vol 100 ◽  
pp. 18-26 ◽  
Author(s):  
A.A. Abdel-Fattah ◽  
W.B. Beshir ◽  
H.M. Hassan ◽  
Y.S. Soliman
Keyword(s):  
Low Dose ◽  
Nitro Blue Tetrazolium ◽  
Gel Dosimeter ◽  
Blue Tetrazolium ◽  
Radiation Induced

Transformation of neutrophils as indicator of irreversibility in hemorrhagic shock

1989 ◽  
Vol 257 (3) ◽  
pp. H846-H852 ◽  
Author(s):  
J. Barroso-Aranda ◽  
G. W. Schmid-Schonbein
Keyword(s):  
Survival Rate ◽  
Arterial Pressure ◽  
Hemorrhagic Shock ◽  
Ischemic Injury ◽  
Nitro Blue Tetrazolium ◽  
Shock Model ◽  
Experimental Procedure ◽  
Spontaneous Reduction ◽  
Blue Tetrazolium ◽  
Low Probability

Recent evidence shows that the presence of circulating neutrophils (PMNs) influences the lethal course and extent of ischemic injury associated with hemorrhagic shock. The objective of this study was to use the Wiggers shock model to investigate the correlation between the appearance of activated circulating PMNs as measured by the spontaneous reduction of nitro blue tetrazolium [(NBT)-positive PMNs] and survival rate. Rats were maintained at 35-mmHg arterial pressure for 90 min and then observed over 24 h for survival. This protocol resulted in 50% mortality. In all animals in which levels of circulating NBT-positive PMNs before bleeding were greater than 225 cells/mm3, the experimental procedure was lethal. Animals that initially had low numbers of NBT-positive PMNs (to approximately 135 cells/mm3) and maintained low counts during the hypotensive period consistently survived. Animals, which before bleeding had low NBT-positive PMN counts but during the course of hypotension significantly elevated their circulating count, have low probability for survival. In those cases in which high NBT-positive PMN counts occurred only later in the course of the hypotensive period, the rats survived. These results suggest that the number of circulating NBT-positive PMNs is a singular index for the trend toward irreversibility in this shock model.

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