scholarly journals A Novel Strategy for Growth Suppression of Glioma Cells: Targeting Microtubules

2004 ◽  
Vol 37 (3) ◽  
pp. 153-158
Author(s):  
Akira Matsuno ◽  
Tadashi Nagashima
Keyword(s):  
Glioma Cells ◽  
Growth Suppression ◽  
Novel Strategy

scholarly journals Growth suppression of glioma cells by PTEN requires a functional phosphatase catalytic domain

1997 ◽  
Vol 94 (23) ◽  
pp. 12479-12484 ◽  
Author(s):  
F. B. Furnari ◽  
H. Lin ◽  
H.- J. S. Huang ◽  
W. K. Cavenee
Keyword(s):  
Catalytic Domain ◽  
Glioma Cells ◽  
Growth Suppression

Growth suppression and astrocytic differentiation of glioma cells by interleukin-1

1994 ◽  
Vol 81 (3) ◽  
pp. 402-410 ◽  
Author(s):  
Satoshi Tanaka ◽  
Tadashi Nagashima ◽  
Shinya Manaka ◽  
Tomokatsu Hori ◽  
Shigeru Yasumoto
Keyword(s):  
Cell Growth ◽  
Cell Lines ◽  
Glioma Cell ◽  
Interleukin 1 ◽  
Glioma Cells ◽  
Growth Suppression ◽  
Transient Growth ◽  
Glioma Cell Lines ◽  
Astrocytic Differentiation

✓ The effect of recombinant human interleukin-1 (rHuIL-1) derivatives on human glioma cell lines was examined in vitro. Five glioma cell lines, U-251 MG, U-373 MG, U-87 MG, A-172, and T98G, were incubated in medium containing 1% fetal calf serum and various concentrations of different types of rHuIL-1: OCT-43 (rHuIL-1β), OCT-7000 (rHuIL-1α), and OCT-8000 (rHuIL-1α). The high-affinity IL-1 receptors were expressed in the U-251 MG and U-373 MG cell lines, and rHuIL-1 was found to suppress cell growth and to induce morphological differentiation of these cell lines. Growth inhibition occurred in a dose-dependent manner in concentrations or rHuIL-1 ranging between 1 and 100 ng/ml. Interestingly, rHuIL-1 induced a transient growth of glioma cells shortly after administration, then suppressed cell growth with accompanying elongation of cytoplasmic processes. This unique process of transient growth stimulation followed by growth suppression was parallel to the efficacy of bromodeoxyuridine uptake in the rHuIL-1-treated cells. Concomitantly, accumulation of glial fibrillary acidic protein and cyclic adenosine monophosphate contents was observed in four glioma cell lines. Continuous rHuIL-1 treatment for longer than 30 days elicited irreversible astrocytic terminal differentiation. These results indicate that IL-1 is an effector on the growth regulation of glioma cells, resulting in astrocytic differentiation in vitro.

scholarly journals Effect of DcR3-specific siRNA on cell growth suppression and apoptosis induction in glioma cells via affecting ERK and AKT

2016 ◽  
Vol Volume 9 ◽  
pp. 5195-5202 ◽  
Author(s):  
Zhuxin Wei ◽  
Yu Zhang ◽  
Suning Huang ◽  
Yuhua Leng ◽  
Xin Chen ◽  
...  
Keyword(s):  
Cell Growth ◽  
Glioma Cells ◽  
Growth Suppression ◽  
Apoptosis Induction ◽  
Cell Growth Suppression

scholarly journals Growth suppression activity of bradykinin antagonists in glioma cells

2014 ◽  
Vol 30 (1) ◽  
pp. 77-79 ◽  
Author(s):  
S. S. Avdieiev ◽  
L. Gera ◽  
R. Hodges ◽  
Y. S. Vassetzky ◽  
V. M. Kavsan
Keyword(s):  
Glioma Cells ◽  
Growth Suppression ◽  
Bradykinin Antagonists

scholarly journals Biosynthetic circ-PLEKHA5 stabilized by SRSF7 promotes the vasculogenic mimicry of glioblastoma cells by encoding a novel protein 622aa

2020 ◽  
Author(s):  
Rui Su ◽  
Xiaobai Liu ◽  
Hao Teng ◽  
Xuelei Ruan ◽  
Di Wang ◽  
...  
Keyword(s):  
Vasculogenic Mimicry ◽  
Glioma Cells ◽  
Immunofluorescence Assay ◽  
Luciferase Assay ◽  
Migration And Invasion ◽  
Glioblastoma Cells ◽  
Associated Proteins ◽  
Novel Strategy ◽  
Novel Protein

Abstract Background: Increasing studies have been demonstrated that circRNAs play vital regulatory roles in the biological behaviors of glioblastoma cells, and increasing circRNAs are found to have the capacity of encoding small peptides, which are involved in the regulatory process.Methods: Western blot and qRT-PCR were conducted to confirm the expression of SRSF7 and circ-PLEKHA5 respectively. RNase R digestion assay and fluorescence in situ hybridization assays were conducted to evaluate the existence and cellular location of circ-PLEKHA5. RIP assay was used to access the relationship between SRSF7 and circ-PLEKHA5. Dual-luciferase assay and FLAG tag assays were performed to test the coding capability of circ-PLEKHA5. Immunofluorescence assay was conducted to evaluate the location of circ-PLEKHA5-622aa. CCK-8, vasculogenic mimicry (VM) formation and transwell assays were used to evaluate the roles of SRSF7, circ-PLEKHA5, circ-PLEKHA5-622aa on proliferation, VM formation, migration and invasion. Nude mice xenograft studys with PAS-CD34 staining were used to clarify the functional roles of SRSF7 and circ-PLEKHA5 on VM formation in vivo.Results: SRSF7 was up-regulated in glioma, and promoted the proliferation, migration, invasion, VM formation and the expression of VM-associated proteins of glioma cells by increasing the expression of circ-PLEKHA5. Circ-PLEKHA5 was mainly localized in cytoplasm and promoted the proliferation, migration, invasion, VM formation and the expression of VM-associated proteins of glioma cells by encoding a novel protein circ-PLEKHA5-622aa. The application of SRSF7 and circ-PLEKHA5 inhibitor significantly suppressed the tumor growth and VM formation in vivo.Conclusions: This study first demonstrated the coding ability of circ-PLEKHA5, and identified the regulatory roles of SRSF7/circ-PLEKHA5/circ-PLEKHA5-622aa pathway in VM formation of glioblastoma cells. Our findings might provide a novel strategy for glioma treatment.

scholarly journals Nimotuzumab enhances temozolomide‐induced growth suppression of glioma cells expressing mutant EGFR in vivo

2016 ◽  
Vol 5 (3) ◽  
pp. 486-499 ◽  
Author(s):  
Yusuke Nitta ◽  
Saki Shimizu ◽  
Yukiko Shishido‐Hara ◽  
Kaori Suzuki ◽  
Yoshiaki Shiokawa ◽  
...  
Keyword(s):  
Glioma Cells ◽  
Growth Suppression ◽  
Mutant Egfr

scholarly journals Effect of TNFRSF6B neutralization antibody on cell growth suppression and apoptosis induction in glioma cells

Neoplasma ◽  
2015 ◽  
Vol 62 (04) ◽  
pp. 574-581 ◽  
Author(s):  
Y. RUAN ◽  
S. HUANG ◽  
D. HE ◽  
R. GOPAUL ◽  
Z. LI ◽  
...  
Keyword(s):  
Cell Growth ◽  
Glioma Cells ◽  
Growth Suppression ◽  
Apoptosis Induction ◽  
Cell Growth Suppression

scholarly journals Infectious delivery of the 132 kb CDKN2A/CDKN2B genomic DNA region results in correctly spliced gene expression and growth suppression in glioma cells

Gene Therapy ◽  
2004 ◽  
Vol 11 (15) ◽  
pp. 1195-1204 ◽  
Author(s):  
R Inoue ◽  
K A Moghaddam ◽  
M Ranasinghe ◽  
Y Saeki ◽  
E A Chiocca ◽  
...  
Keyword(s):  
Gene Expression ◽  
Genomic Dna ◽  
Glioma Cells ◽  
Growth Suppression
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