scholarly journals The Relationship between Numerical Aberrations of Chromosome 17 and Nuclear DNA Content in Colorectal Carcinoma Detected by Fluorescent In Situ Hybridization (FISH) and Cytofluorometry Using Auto-scanning Stage.

1997 ◽  
Vol 30 (5/6) ◽  
pp. 665-673
Author(s):  
Kanji Kawai ◽  
Shinshichi Hamada ◽  
Hisakazu Yamagishi ◽  
Hirosumi Itoi ◽  
Toshiya Ochiai ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Colorectal Carcinoma ◽  
Dna Content ◽  
Fluorescent In Situ Hybridization ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Chromosome 17 ◽  
Numerical Aberrations ◽  
The Relationship

Chromatin characterization by banding techniques, in situ hybridization, and nuclear DNA content in Cicer L. (Leguminosae)

Genome ◽  
1996 ◽  
Vol 39 (2) ◽  
pp. 258-265 ◽  
Author(s):  
I. Galasso ◽  
D. Pignone ◽  
M. Frediani ◽  
M. Maggiani ◽  
R. Cremonini
Keyword(s):  
In Situ Hybridization ◽  
Dna Content ◽  
Nuclear Dna ◽  
Hybrid Sterility ◽  
Nuclear Dna Content ◽  
5S Rdna ◽  
Rrna Genes ◽  
Evolutionary Pathway ◽  
C Banding

The karyotypes of three accessions, one each from three annual species of the genus Cicer, namely Cicer arietinum, Cicer reticulation, and Cicer echinospermum, were examined and compared using C-banding, the fluorochromes chromomycin A3, DAPI, and Hoechst 33258, in situ hybridization of the 18S–5.8S–25S and 5S rDNA sequences, and silver staining. The nuclear DNA content of the three species and the amount of heterochromatin were also determined. The results suggest an evolutionary pathway in which C. reticulatum is the ancestral species from which both C. arietinum and C. echinospermum are derived with the loss of one pair of satellites; subsequently, C. echinospermum further differentiated by the accumulation of chromosomal rearrangement(s) that gave rise to a hybrid sterility barrier. Key words : Cicer, C-banding, fluorochromes, Ag staining, rRNA genes.

Determination of nuclear DNA content in fungi using mithramycin: vegetative diploidy in Armillaria mellea confirmed

1983 ◽  
Vol 29 (9) ◽  
pp. 1179-1183 ◽  
Author(s):  
A. L. Franklin ◽  
W. G. Filion ◽  
J. B. Anderson
Keyword(s):  
Dna Binding ◽  
Aspergillus Nidulans ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Phytopathogenic Fungus ◽  
Armillaria Mellea ◽  
Dna Measurements

Armillaria mellea, a phytopathogenic fungus, is the only member of the Agaricales (Basidiomycetes) whose fertile vegetative phase in nature is thought to be diploid, rather than dikaryotic. To examine the vegetative ploidy of A. mellea, we used the DNA-binding antibiotic, mithramycin, for fluorometry of in situ nuclear DNA. The measurements of nuclear DNA content indicated that strains derived from single basidiospores of A. mellea were haploid and that strains derived from matings of isolates of single spores were diploid. These data confirm the results of earlier genetic experiments, which show haploidy and diploidy in unmated and mated strains, respectively. Nuclear DNA measurements in known haploid and diploid strains of Aspergillus nidulans confirmed the validity of our protocol.

DNA content in Tradescantia

1985 ◽  
Vol 27 (6) ◽  
pp. 766-775 ◽  
Author(s):  
Arturo Martínez ◽  
Héctor D. Ginzo
Keyword(s):  
North America ◽  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Life Forms ◽  
Chromosome Size ◽  
Polyploid Complex ◽  
Ploidy Levels ◽  
Vegetative Adaptation ◽  
The Relationship

There is a wide variation in the nuclear DNA content and chromosome size between the species belonging to the T. crassifolia and T. virginiana alliances (all the species but one are native to Central and North America). Also the DNA content per genome decreases when the ploidy level increases within the same specific polyploid complex with three ploidy levels (2x, 4x, and 6x). In contrast, no variation was found in the DNA content per genome between different ploidy levels in the T. fluminensis alliance (all the species are native to South America) where they range from 6x to 22x. Since all the species described here are perennials with various life forms, it was possible to analyze the relationship between the DNA content and their vegetative adaptation to the environment. The more specialized species (geophytes and hemicryptophytes) have a higher amount of DNA than the chamaephytes adapted to live in relatively more mesic regions. In the species living in Central and North America there is a positive correlation between the increase in DNA content and the latitude of their native regions.Key words: Tradescantia, DNA content, geographical distribution, life forms, polyploidy.

Osteocyte lacunae size in the genus Xenopus (Pipidae)

1987 ◽  
Vol 8 (4) ◽  
pp. 315-320 ◽  
Author(s):  
Jelle W.F. Reumer ◽  
Charles-H. Thiébaud
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Strongly Correlated ◽  
Osteocyte Lacunae ◽  
The Relationship

AbstractThe relationship is studied between the volume of osteocyte lacunae and the nuclear DNA content and level of ploidy in the genus Xenopus. The lacunae volume and the DNA content appear strongly correlated. The possible use of this relationship in the study of the evolution of the genus is discussed.

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