Study of numerical aberrations of chromosome 1 by fluorescent in situ hybridization and DNA content by densitometric analysis on (pre)-malignant cervical lesions

1995 ◽  
Vol 27 (1) ◽  
pp. 24-34 ◽  
Author(s):  
Pascale Segers ◽  
Sonja Haesen ◽  
Philippe Castelain ◽  
Jean-Jaques Amy ◽  
Philippe De Sutter ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Content ◽  
Fluorescent In Situ Hybridization ◽  
Chromosome 1 ◽  
Cervical Lesions ◽  
Densitometric Analysis ◽  
Numerical Aberrations

Recovery of a telomere-truncated chromosome via a compensating translocation in maize

Genome ◽  
2011 ◽  
Vol 54 (3) ◽  
pp. 184-195 ◽  
Author(s):  
Robert T. Gaeta ◽  
Tatiana V. Danilova ◽  
Changzeng Zhao ◽  
Rick E. Masonbrink ◽  
Morgan E. McCaw ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescent In Situ Hybridization ◽  
Transgene Expression ◽  
De Novo ◽  
Extra Chromosome ◽  
Single Gene ◽  
B Chromosomes ◽  
Chromosome 1 ◽  
Chromosome 6

Maize-engineered minichromosomes are easily recovered from telomere-truncated B chromosomes but are rarely recovered from A chromosomes. B chromosomes lack known genes, and their truncation products are tolerated and transmitted during meiosis. In contrast, deficiency gametes resulting from truncated A chromosomes prevent their transmission. We report here a de novo compensating translocation that permitted recovery of a large truncation of chromosome 1 in maize. The truncation (trunc-1) and translocation with chromosome 6 (super-6) occurred during telomere-mediated truncation experiments and were characterized using single-gene fluorescent in situ hybridization (FISH) probes. The truncation contained a transgene signal near the end of the broken chromosome and transmitted together with the compensating translocation as a heterozygote to approximately 41%–55% of progeny. Transmission as an addition chromosome occurred in ~15% of progeny. Neither chromosome transmitted through pollen. Transgene expression (Bar) cosegregated with trunc-1 transcriptionally and phenotypically. Meiosis in T1 plants revealed eight bivalents and one tetravalent chain composed of chromosome 1, trunc-1, chromosome 6, and super-6 in diplotene and diakinesis. Our data suggest that de novo compensating translocations allow recovery of truncated A chromosomes by compensating deficiency in female gametes and by affecting chromosome pairing and segregation. The truncated chromosome can be maintained as an extra chromosome or together with the super-6 as a heterozygote.

DNA Content Measurement and In Situ Hybridization in Condylomatous Cervical Lesions

1992 ◽  
Vol 1 (1) ◽  
pp. 180-184 ◽  
Author(s):  
Christine Clavel ◽  
Laurent Zerat ◽  
Isabelle Binninger ◽  
Marie-Claude Boutterin ◽  
Myriam Polette ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Dna Content ◽  
Cervical Lesions ◽  
Content Measurement

scholarly journals Analysis of numerical aberrations in specific chromosomes by fluorescent in situ hybridization as a diagnostic tool in breast cancer

Cancer ◽  
1996 ◽  
Vol 77 (10) ◽  
pp. 2064-2069 ◽  
Author(s):  
Daisuke Ichikawa ◽  
Naoya Hashimoto ◽  
Masakazu Hoshima ◽  
Toshiharu Yamaguchi ◽  
Kiyoshi Sawai ◽  
...  
Keyword(s):  
Breast Cancer ◽  
In Situ Hybridization ◽  
Diagnostic Tool ◽  
Fluorescent In Situ Hybridization ◽  
Numerical Aberrations

The recurrent IgH translocations are highly associated with nonhyperdiploid variant multiple myeloma

Blood ◽  
2003 ◽  
Vol 102 (7) ◽  
pp. 2562-2567 ◽  
Author(s):  
Rafael Fonseca ◽  
Carina S. Debes-Marun ◽  
Elisa B. Picken ◽  
Gordon W. Dewald ◽  
Sandra C. Bryant ◽  
...  
Keyword(s):  
Multiple Myeloma ◽  
In Situ Hybridization ◽  
Heavy Chain ◽  
Dna Content ◽  
Mayo Clinic ◽  
Fluorescent In Situ Hybridization ◽  
Eastern Cooperative Oncology Group ◽  
Oncology Group

Abstract Aneuploid is ubiquitous in multiple myeloma (MM), and 4 cytogenetic subcategories are recognized: hypodiploid (associated with a shorter survival), pseudodiploid, hyperdiploid, and near-tetraploid MM. The hypodiploid, pseudodiploid, and near-tetraploid karyotypes can be referred to as the nonhyperdiploid MM. Immunoglobulin heavy-chain (IgH) translocations are seen in 60% of patients. We studied the relation between aneuploidy and IgH translocations in MM. Eighty patients with MM and abnormal metaphases were studied by means of interphase fluorescent in situ hybridization (FISH) to detect IgH translocations. We also studied a second cohort of 199 patients (Eastern Cooperative Oncology Group [ECOG]) for IgH translocations, chromosome 13 monosomy/deletions (Δ13), and ploidy by DNA content. Mayo Clinic patients with abnormal karyotypes and FISH-detected IgH translocation were more likely to be nonhyperdiploid (89% versus 39%, P < .0001). Remarkably, 88% of tested patients with hypodiploidy (16 of 18) and 90% of tested patients with tetraploidy (9 of 10) had an IgH translocation. ECOG patients with IgH translocations were more likely to have nonhyperdiploid MM by DNA content (68% versus 21%, P < .001). This association was seen predominantly in patients with recurrent chromosome partners to the IgH translocation (11q13, 4p16, and 16q23). The classification of MM into hyperdiploidy and nonhyperdiploidy is dictated largely by the recurrent (primary) IgH translocations in the latter. (Blood. 2003;102:2562-2567)

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