scholarly journals Expression pattern of the homeobox gene Hox-3.5 during mouse development, as revealed by a simplified in situ hybridization method.

1990 ◽  
Vol 23 (3) ◽  
pp. 353-366 ◽  
Author(s):  
SUMIHARE NOJI ◽  
NAOKI TAKAHASHI ◽  
TSUTOMU NOHNO ◽  
EIKI KOYAMA ◽  
TOMOICHIRO YAMAAI ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Expression Pattern ◽  
Homeobox Gene ◽  
Hybridization Method ◽  
Mouse Development

scholarly journals Region-specific expression in early chick and mouse embryos of Ghox-lab and Hox 1.6, vertebrate homeobox-containing genes related to Drosophila labial

Development ◽  
1990 ◽  
Vol 108 (1) ◽  
pp. 47-58
Author(s):  
O.H. Sundin ◽  
H.G. Busse ◽  
M.B. Rogers ◽  
L.J. Gudas ◽  
G. Eichele
Keyword(s):  
In Situ Hybridization ◽  
Homeobox Gene ◽  
Mouse Embryos ◽  
Mouse Development ◽  
Temporal And Spatial Distribution ◽  
Specific Expression ◽  
Early Embryos ◽  
Close Relationship ◽  
Additional Sequence

A chick gene homologous to the Drosophila homeobox gene labial has been cloned and sequenced. Regions of additional sequence identity outside of the homeobox reveal a close relationship to the mouse gene Hox 1.6. Northern blot analysis demonstrates that Ghox-lab and Hox 1.6 transcripts are both present at high levels during early stages of chick and mouse development, with a subsequent decline in abundance to very low levels by the time limb mesenchyme begins to differentiate. In situ hybridization analysis of chick embryos shows intense expression of Ghox-lab mRNA by Hamburger and Hamilton stage 4 (avian ‘mid gastrula’) and by stage 6 (pre-somitic neural plate) with expression decreasing shortly thereafter. The pattern of Ghox-lab RNA expression in these early embryos divides the embryo into an anterior and a posterior compartment. At stage 6, considerable signal is observed in the posterior two thirds of the embryo, while none is detected in the anterior third which is fated to become the head. This pattern is purely regional in nature, and does not follow boundaries defined by known tissue types. In situ hybridization of Hox 1.6 probes to mouse embryos of day 7.5 or 8.0 indicate that the Hox 1.6 transcript has a temporal and spatial distribution very similar to that of Ghox-lab in the chick embryo.

Comet and cup genes in Drosophila spermatogenesis: the first demonstration of post-meiotic transcription

2008 ◽  
Vol 36 (3) ◽  
pp. 540-542 ◽  
Author(s):  
Carine Barreau ◽  
Elizabeth Benson ◽  
Helen White-Cooper
Keyword(s):  
In Situ Hybridization ◽  
Expression Pattern ◽  
Reverse Transcription ◽  
Protein Product ◽  
Rt Pcr ◽  
Pcr Assay ◽  
Single Cyst ◽  
Reverse Transcription Pcr

Post-meiotic transcription is widespread in mammalian spermatogenesis, but is generally believed to be absent from Drosophila spermatogenesis. Genes required during meiosis, in early spermatids or later in spermiogenesis are typically transcribed in primary spermatocytes in Drosophila. Their mRNAs are then stored in the cytoplasm until the protein product is needed. Recently, using in situ hybridization, we identified 17 Drosophila genes, collectively named ‘comets’ and ‘cups’, whose mRNAs are most abundant in, and localize to the distal ends of, elongating spermatids. Using a single-cyst quantitative RT–PCR (reverse transcription–PCR) assay, we confirmed this unusual expression pattern and conclusively demonstrate the existence of post-meiotic transcription in Drosophila spermatids. We found that transcription of comets and cups occurs just before protamines can be detected in spermatid nuclei.

Prealbumin gene expression during mouse development studied by in situ hybridization

1987 ◽  
Vol 22 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Tatsufumi Murakami ◽  
Yoshiaki Yasuda ◽  
Shuji Mita ◽  
Shuichiro Maeda ◽  
Kazunori Shimada ◽  
...  
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Mouse Development

scholarly journals Formaldehyde-based whole-mount in situ hybridization method for planarians

2009 ◽  
Vol 238 (2) ◽  
pp. 443-450 ◽  
Author(s):  
Bret J. Pearson ◽  
George T. Eisenhoffer ◽  
Kyle A. Gurley ◽  
Jochen C. Rink ◽  
Diane E. Miller ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Hybridization Method ◽  
Whole Mount

Rapid Diagnosis of Legionella Infection by a Nonisotopic In Situ Hybridization Method

1991 ◽  
Vol 95 (5) ◽  
pp. 719-724 ◽  
Author(s):  
Jonathan S. Fain ◽  
Robert N. Bryan ◽  
Lorna Cheng ◽  
Klaus J. Lewin ◽  
David D. Porter ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Rapid Diagnosis ◽  
Hybridization Method
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