scholarly journals Immunohistochemical study of .GAMMA.-glutamyl transpeptidase during the development and maturation of rat kidney.

1990 ◽  
Vol 23 (2) ◽  
pp. 149-163
Author(s):  
JIAN-ZHAO NIU ◽  
SHUJI YAMASHITA ◽  
KENJIRO YASUDA
Keyword(s):  
Immunohistochemical Study ◽  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

Inhibition of the hydrolytic and transpeptidase activities of rat kidney gamma-glutamyl transpeptidase by specific monoclonal antibodies

1999 ◽  
Vol 260 (3) ◽  
pp. 844-854 ◽  
Author(s):  
Evgenia Bluvshtein ◽  
George A. Glass ◽  
Gloria Volohonsky ◽  
Margalit Yaakubowitz ◽  
Ella Harness ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

scholarly journals A monoclonal antibody against human kidney gamma-glutamyl transpeptidase: preparation, immunochemical, and immunohistochemical characterization.

1989 ◽  
Vol 37 (7) ◽  
pp. 1053-1061 ◽  
Author(s):  
M Shiozawa ◽  
S Yamashita ◽  
S Aiso ◽  
K Yasuda
Keyword(s):  
Monoclonal Antibody ◽  
Immunohistochemical Study ◽  
Basolateral Membrane ◽  
Human Kidney ◽  
Normal Liver ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Human Organs ◽  
Human Enzyme ◽  
Glutamyl Transpeptidase

To perform immunohistochemical study of the distribution of gamma-glutamyl transpeptidase in human organs, a highly specific antibody against the human enzyme is required. We prepared monoclonal antibody against gamma-glutamyl transpeptidase from human kidney, using the hybridoma technique. The antibody was of the IgG1 type and the light chain belonged to the kappa class. The antibody reacted specifically with the 63 KD heavy subunit of the enzyme. Examination of the specificity of the antibody performed by immunohistochemical staining of human kidney sections revealed that the antigen was localized on the brush border and along the basolateral membrane of the epithelial cells of both the convoluted and the straight portions of the proximal tubule. This antibody was also reactive in several human organs other than kidney, including epididymis, prostate, seminal vesicle, pancreas, and normal liver, and in human hepatoma. These findings indicate the existence of an antigenic determinant common to human kidney and other organs. The monoclonal antibody did not crossreact with mouse, rat, guinea pig, rabbit, or pig kidney.

Identification of a second promoter which drives the expression of .gamma.-glutamyl transpeptidase in rat kidney and epididymis

Biochemistry ◽  
1992 ◽  
Vol 31 (38) ◽  
pp. 9190-9196 ◽  
Author(s):  
Olivier Lahuna ◽  
Arthur Brouillet ◽  
Marie Noelle Chobert ◽  
Mojtaba Darbouy ◽  
Tomomitsu Okamoto ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

scholarly journals Molecular cloning and nucleotide sequence of rat kidney gamma-glutamyl transpeptidase cDNA.

1986 ◽  
Vol 83 (4) ◽  
pp. 937-941 ◽  
Author(s):  
Y. Laperche ◽  
F. Bulle ◽  
T. Aissani ◽  
M. N. Chobert ◽  
M. Aggerbeck ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Molecular Cloning ◽  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase
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