scholarly journals Immunohistochemical study of gamma-glutamyl transpeptidase with monoclonal antibodies. II. An immunoelectron microscopic study in rat kidney.

1989 ◽  
Vol 22 (3) ◽  
pp. 367-374 ◽  
Author(s):  
SHUJI YAMASHITA ◽  
SADAKAZU AISO ◽  
MASAHIDE SHIOZAWA ◽  
KENJIRO YASUDA
Keyword(s):  
Monoclonal Antibodies ◽  
Microscopic Study ◽  
Immunohistochemical Study ◽  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

Inhibition of the hydrolytic and transpeptidase activities of rat kidney gamma-glutamyl transpeptidase by specific monoclonal antibodies

1999 ◽  
Vol 260 (3) ◽  
pp. 844-854 ◽  
Author(s):  
Evgenia Bluvshtein ◽  
George A. Glass ◽  
Gloria Volohonsky ◽  
Margalit Yaakubowitz ◽  
Ella Harness ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

scholarly journals A monoclonal antibody against human kidney gamma-glutamyl transpeptidase: preparation, immunochemical, and immunohistochemical characterization.

1989 ◽  
Vol 37 (7) ◽  
pp. 1053-1061 ◽  
Author(s):  
M Shiozawa ◽  
S Yamashita ◽  
S Aiso ◽  
K Yasuda
Keyword(s):  
Monoclonal Antibody ◽  
Immunohistochemical Study ◽  
Basolateral Membrane ◽  
Human Kidney ◽  
Normal Liver ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Human Organs ◽  
Human Enzyme ◽  
Glutamyl Transpeptidase

To perform immunohistochemical study of the distribution of gamma-glutamyl transpeptidase in human organs, a highly specific antibody against the human enzyme is required. We prepared monoclonal antibody against gamma-glutamyl transpeptidase from human kidney, using the hybridoma technique. The antibody was of the IgG1 type and the light chain belonged to the kappa class. The antibody reacted specifically with the 63 KD heavy subunit of the enzyme. Examination of the specificity of the antibody performed by immunohistochemical staining of human kidney sections revealed that the antigen was localized on the brush border and along the basolateral membrane of the epithelial cells of both the convoluted and the straight portions of the proximal tubule. This antibody was also reactive in several human organs other than kidney, including epididymis, prostate, seminal vesicle, pancreas, and normal liver, and in human hepatoma. These findings indicate the existence of an antigenic determinant common to human kidney and other organs. The monoclonal antibody did not crossreact with mouse, rat, guinea pig, rabbit, or pig kidney.

Identification of a second promoter which drives the expression of .gamma.-glutamyl transpeptidase in rat kidney and epididymis

Biochemistry ◽  
1992 ◽  
Vol 31 (38) ◽  
pp. 9190-9196 ◽  
Author(s):  
Olivier Lahuna ◽  
Arthur Brouillet ◽  
Marie Noelle Chobert ◽  
Mojtaba Darbouy ◽  
Tomomitsu Okamoto ◽  
...  
Keyword(s):  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Gamma Glutamyl ◽  
Glutamyl Transpeptidase

scholarly journals Monoclonal antibodies against rat kidney γ-glutamyl transpeptidase show species and tissue specificity

1986 ◽  
Vol 238 (3) ◽  
pp. 913-917 ◽  
Author(s):  
J A Green ◽  
N D Cook ◽  
M M Manson
Keyword(s):  
Monoclonal Antibodies ◽  
Brush Border ◽  
Tissue Specificity ◽  
Rat Kidney ◽  
Gamma Glutamyl Transpeptidase ◽  
Brush Border Membranes ◽  
Adult Rat ◽  
Foetal Rat ◽  
Epithelial Tumours ◽  
Glutamyl Transpeptidase

Monoclonal antibodies have been raised against rat kidney gamma-glutamyl transpeptidase (GGT). All five antibodies immunoprecipitate enzyme activity from solubilized kidney brush-border membranes, but not from hepatocellular carcinoma membranes. Three of the antibodies react immunohistochemically with brush-border membranes in sections of adult rat kidney, but none of the antibodies cross-react with sections of guinea-pig, mouse or marmoset kidney or with untreated or carcinogen-treated rat liver. The antibodies do not recognize GGT in foetal-rat kidney and react poorly with kidney from 2-year-old rat. They do react with tubules trapped within mesenchymal kidney tumours induced by dimethylnitrosamine, but epithelial tumours, which are GGT-positive (although much less so than normal kidney), are not immunoreactive.

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