scholarly journals Modulation of αvβ3 Integrin via Transactivation of β3 Integrin Gene on Murine Bone Marrow Macrophages by 1,25(OH)2D3, Retinoic Acid and Interleukin-4

2019 ◽  
Vol 52 (4) ◽  
pp. 77-83 ◽  
Author(s):  
Sohei Kitazawa ◽  
Ryuma Haraguchi ◽  
Yukihiro Kohara ◽  
Riko Kitazawa
Keyword(s):  
Bone Marrow ◽  
Retinoic Acid ◽  
Interleukin 4 ◽  
Murine Bone Marrow ◽  
Integrin Gene

scholarly journals Enterococcus faecalis Induces Differentiation of Immune-Aberrant Dendritic Cells from Murine Bone Marrow-Derived Stem Cells

2020 ◽  
Vol 88 (11) ◽  
Author(s):  
Mohamed Mohamed Elashiry ◽  
Mahmoud Elashiry ◽  
Rana Zeitoun ◽  
Ranya Elsayed ◽  
Fucong Tian ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Dendritic Cells ◽  
Enterococcus Faecalis ◽  
Transforming Growth Factor ◽  
Interleukin 4 ◽  
Dependent Manner ◽  
Immune Functions ◽  
Content Type ◽  
Murine Bone Marrow

ABSTRACT Enterococcus faecalis, long implicated in serious systemic infections and failure of root canal treatment, is a persistent inhabitant of oral periapical lesions. Dendritic cells (DCs) and other innate immune cells patrol the oral mucosa for infecting microbes. Dendritic cells are efficient at capturing microbes when immature, whereupon they can transform into potent antigen-presenting cells upon full maturation. Autophagy, a sophisticated intracellular process first described for elimination of damaged organelles, regulates DC maturation and other important immune functions of DCs. The present study examined how E. faecalis influences the differentiation of murine bone marrow-derived stem cells (BMSCs) into functional DCs in the presence of the cytokines granulocyte-macrophage colony-stimulating factor (GM-CSF) and interleukin-4 (IL-4). Although the viability and differentiation of DCs were not affected by E. faecalis, expression of the autophagy-related proteins ATG7, Beclin1, and LC3bI/II were significantly suppressed in an mTOR-dependent manner. Ultrastructurally, E. faecalis was identified in single-membrane vacuoles, some of which were in the process of binary fission. Bacterium-containing autophagosomes were absent within the cytoplasm. Accessory molecules (major histocompatibility complex class II [MHC-II], CD80, and CD86) and anti-inflammatory cytokine (transforming growth factor β1 [TGF-β1]) were suppressed in E. faecalis-induced DCs, while IL-1β, tumor necrosis factor alpha (TNF-α), and IL-12 levels were upregulated. When pulsed with ovalbumin (OVA), the E. faecalis-induced DCs showed reduction in CD4+ OVA-specific OT-II T cell proliferation. It is concluded that E. faecalis promotes the differentiation of bone marrow stem cells into CD11c-positive DCs with aberrant immune functions while retaining the capability of proinflammatory cytokine induction.

Interleukin 4, interferon-gamma, and prostaglandin E impact the osteoclastic cell-forming potential of murine bone marrow macrophages.

Endocrinology ◽  
1995 ◽  
Vol 136 (6) ◽  
pp. 2367-2376 ◽  
Author(s):  
D L Lacey ◽  
J M Erdmann ◽  
S L Teitelbaum ◽  
H L Tan ◽  
J Ohara ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Interferon Gamma ◽  
Interleukin 4 ◽  
Prostaglandin E ◽  
Murine Bone Marrow ◽  
Osteoclastic Cell

Author response for "CXCR4, but not CXCR3, drives CD8 T-cell entry into and migration through the murine bone marrow"

2018 ◽  
Author(s):  
Marieke Goedhart ◽  
Stephanie Gessel ◽  
Robbert van der Voort ◽  
Edith Slot ◽  
Beth Lucas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Cd8 T Cell ◽  
Cell Entry ◽  
Author Response ◽  
Murine Bone Marrow ◽  
And Migration
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