Metaphase preparation from murine bone marrow

2008 ◽  
Author(s):  
Wei Guo ◽  
Hong Wu
Keyword(s):  
Bone Marrow ◽  
Murine Bone Marrow

Author response for "CXCR4, but not CXCR3, drives CD8 T-cell entry into and migration through the murine bone marrow"

2018 ◽  
Author(s):  
Marieke Goedhart ◽  
Stephanie Gessel ◽  
Robbert van der Voort ◽  
Edith Slot ◽  
Beth Lucas ◽  
...  
Keyword(s):  
Bone Marrow ◽  
T Cell ◽  
Cd8 T Cell ◽  
Cell Entry ◽  
Author Response ◽  
Murine Bone Marrow ◽  
And Migration

scholarly journals Phagocytosis and activation of bone marrow‐derived macrophages by Plasmodium falciparum gametocytes

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Yolanda Corbett ◽  
Silvia Parapini ◽  
Federica Perego ◽  
Valeria Messina ◽  
Serena Delbue ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Bone Marrow ◽  
Plasmodium Falciparum ◽  
Innate Immune ◽  
Innate Immune Responses ◽  
Murine Bone Marrow ◽  
Inflammatory Protein ◽  
Life Cycle Stages ◽  
Cytokine Levels ◽  
Necrosis Factor

Abstract Background The innate immune response against various life cycle stages of the malaria parasite plays an important role in protection against the disease and regulation of its severity. Phagocytosis of asexual erythrocytic stages is well documented, but little and contrasting results are available about phagocytic clearance of sexual stages, the gametocytes, which are responsible for the transmission of the parasites from humans to mosquitoes. Similarly, activation of host macrophages by gametocytes has not yet been carefully addressed. Methods Phagocytosis of early or late Plasmodium falciparum gametocytes was evaluated through methanol fixed cytospin preparations of immortalized mouse C57Bl/6 bone marrow-derived macrophages treated for 2 h with P. falciparum and stained with Giemsa, and it was confirmed through a standardized bioluminescent method using the transgenic P. falciparum 3D7elo1-pfs16-CBG99 strain. Activation was evaluated by measuring nitric oxide or cytokine levels in the supernatants of immortalized mouse C57Bl/6 bone marrow-derived macrophages treated with early or late gametocytes. Results The results showed that murine bone marrow-derived macrophages can phagocytose both early and late gametocytes, but only the latter were able to induce the production of inflammatory mediators, specifically nitric oxide and the cytokines tumour necrosis factor and macrophage inflammatory protein 2. Conclusions These results support the hypothesis that developing gametocytes interact in different ways with innate immune cells of the host. Moreover, the present study proposes that early and late gametocytes act differently as targets for innate immune responses.

scholarly journals Strain difference of murine bone marrow-derived mast cell functions

2005 ◽  
Vol 78 (3) ◽  
pp. 605-611 ◽  
Author(s):  
Junko Noguchi ◽  
Etsushi Kuroda ◽  
Uki Yamashita
Keyword(s):  
Bone Marrow ◽  
Mast Cell ◽  
Strain Difference ◽  
Murine Bone Marrow ◽  
Cell Functions

p53-dependent and independent apoptosis induced by ionizing radiation in murine bone marrow cells

1997 ◽  
Vol 42 (2) ◽  
pp. 155-159
Author(s):  
Yufang Cui ◽  
Pingkun Zhou ◽  
Brian I. Lord ◽  
Jolyon H. Hendry
Keyword(s):  
Bone Marrow ◽  
Ionizing Radiation ◽  
Bone Marrow Cells ◽  
Murine Bone Marrow ◽  
Marrow Cells
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