scholarly journals Cyclin G2 Is Involved in the Proliferation of Placental Trophoblast Cells and Their Interactions with Endothelial Cells

2020 ◽  
Vol 26 ◽  
Author(s):  
Manni Sun ◽  
Shenghuan Liu ◽  
Jinlan Gao ◽  
Tao Meng ◽  
Xuesha Xing ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Trophoblast Cells ◽  
Cyclin G2

scholarly journals The role of nitric oxide in the outgrowth of trophoblast cells on human umbilical vein endothelial cells

2015 ◽  
Vol 54 (3) ◽  
pp. 227-231 ◽  
Author(s):  
Kuan-Hao Tsui ◽  
Hsin-Yang Li ◽  
Jiin-Tsuey Cheng ◽  
Yen-Jen Sung ◽  
Ming-Shyen Yen ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Endothelial Cells ◽  
Umbilical Vein ◽  
Trophoblast Cells ◽  
Human Umbilical Vein ◽  
Umbilical Vein Endothelial Cells

The effects of vascular endothelial cells on migration of trophoblast cells

Placenta ◽  
2013 ◽  
Vol 34 (10) ◽  
pp. A13
Author(s):  
Tomoko Izawa ◽  
Keiji Sakai ◽  
Mitutoshi Iwashita
Keyword(s):  
Endothelial Cells ◽  
Vascular Endothelial Cells ◽  
Vascular Endothelial ◽  
Trophoblast Cells

scholarly journals Uvaol Prevents Group B Streptococcus-Induced Trophoblast Cells Inflammation and Possible Endothelial Dysfunction

2021 ◽  
Vol 12 ◽  
Author(s):  
Ana Lucia Mendes Silva ◽  
Elaine Cristina Oliveira Silva ◽  
Rayane Martins Botelho ◽  
Liliane Patricia Gonçalves Tenorio ◽  
Aldilane Lays Xavier Marques ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Endothelial Cells ◽  
Cell Line ◽  
Group B Streptococcus ◽  
Trophoblast Invasion ◽  
Trophoblast Cells ◽  
Chorionic Villi ◽  
Group B ◽  
Angiopoietin 2 ◽  
And Function

Group B Streptococcus (GBS) infection during pregnancy is involved in maternal sepsis, chorioamnionitis, prematurity, fetal infection, neonatal sepsis, and neurodevelopmental alterations. The GBS-induced chorioamnionitis leads to a plethora of immune and trophoblast cells alterations that could influence endothelial cells to respond differently to angiogenic mediators and alter placental vascular structure and function in pregnant women. In this context, preventive measures are needed to reduce such dysfunctions. As such, we evaluated the effects of a non-lethal exposure to inactivated GBS on trophoblast cells and chorionic villi explants, and if the treatment with uvaol would mitigate these effects. The concentration of 106 CFU of GBS was chosen since it was unable to reduce the HTR-8/SVneo cell line nor term chorionic villi explant viability. Raman spectroscopy of trophoblast cells showed significant alterations in their biochemical signature, mostly reverted by uvaol. GBS exposure increased HTR-8/SVneo cells IL-1β and IFN-γ production, phagocytosis, oxidative stress, and decreased trophoblast cell migration. The Ea.hy926 endothelial cell line produced angiopoietin-2, CXCL-8, EGF, FGF-b, IL-6, PlGF, sPECAM-1, and VEGF in culture. When co-cultured in invasion assay with HTR-8/SVneo trophoblast cells, the co-culture had increased production of angiopoietin-2, CXCL-8, FGF-b, and VEGF, while reduced sPECAM-1 and IL-6. GBS exposure led to increased CXCL-8 and IL-6 production, both prevented by uvaol. Chorionic villi explants followed the same patterns of production when exposed to GBS and response to uvaol treatment as well. These findings demonstrate that, even a non-lethal concentration of GBS causes placental inflammation and oxidative stress, reduces trophoblast invasion of endothelial cells, and increases CXCL-8 and IL-6, key factors that participate in vascular dysregulation observed in several diseases. Furthermore, uvaol treatment prevented most of the GBS-provoked changes. Hence, uvaol could prevent the harmful effects of GBS infection for both the mother and the fetus.

scholarly journals Role of IL-36 Cytokines in the Regulation of Angiogenesis Potential of Trophoblast Cells

2020 ◽  
Vol 22 (1) ◽  
pp. 285
Author(s):  
José M. Murrieta-Coxca ◽  
Ruby N. Gutiérrez-Samudio ◽  
Heba M. El-Shorafa ◽  
Tanja Groten ◽  
Sandra Rodríguez-Martínez ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Cell Lines ◽  
Cell Types ◽  
Poly I:C ◽  
Synthetic Analog ◽  
Dependent Manner ◽  
Trophoblast Cells ◽  
Trophoblastic Cell ◽  
Mouse Uterus

IL-36 cytokines (the agonists IL-36α, IL-36β, IL-36γ, and the antagonist IL-36Ra) are expressed in the mouse uterus and associated with maternal immune response during pregnancy. Here, we characterize the expression of IL-36 members in human primary trophoblast cells (PTC) and trophoblastic cell lines (HTR-8/SVneo and JEG-3) and upon treatment with bacterial and viral components. Effects of recombinant IL-36 on the migration capacity of trophoblastic cells, their ability to interact with endothelial cells and the induction of angiogenic factors and miRNAs (angiomiRNAs) were examined. Constitutive protein expression of IL-36 (α, β, and γ) and their receptor (IL-36R) was found in all cell types. In PTC, transcripts for all IL-36 subtypes were found, whereas in trophoblastic cell lines only for IL36G and IL36RN. A synthetic analog of double-stranded RNA (poly I:C) and lipopolysaccharide (LPS) induced the expression of IL-36 members in a cell-specific and time-dependent manner. In HTR-8/SVneo cells, IL-36 cytokines increased cell migration and their capacity to interact with endothelial cells. VEGFA and PGF mRNA and protein, as well as the angiomiRNAs miR-146a-3p and miR-141-5p were upregulated as IL-36 response in PTC and HTR-8/SVneo cells. In conclusion, IL-36 cytokines are modulated by microbial components and regulate trophoblast migration and interaction with endothelial cells. Therefore, a fundamental role of these cytokines in the placentation process and in response to infections may be expected.

Sign in / Sign up

Export Citation Format

Share Document