scholarly journals Preparation and Characterization of a Novel Triple Composite Scaffold Containing Silk Fiborin, Chitosan, and Alginate for 3D Culture of Colonic Carcinoma Cells In Vitro

2020 ◽  
Vol 26 ◽  
Author(s):  
Xianhao Su ◽  
Liang Chen ◽  
Shanliang Han ◽  
Gengming Niu ◽  
Jun Ren ◽  
...  
Keyword(s):  
Composite Scaffold ◽  
3D Culture ◽  
Carcinoma Cells ◽  
Colonic Carcinoma

scholarly journals The Use of Porous Scaffold as a Tumor Model

2013 ◽  
Vol 2013 ◽  
pp. 1-9 ◽  
Author(s):  
Mei Zhang ◽  
Philip Boughton ◽  
Barbara Rose ◽  
C. Soon Lee ◽  
Angela M. Hong
Keyword(s):  
Cancer Cells ◽  
Cancer Cell ◽  
Porous Scaffold ◽  
Composite Scaffold ◽  
Human Cancer ◽  
Expression Patterns ◽  
3D Structure ◽  
3D Culture ◽  
Growth Patterns

Background. Human cancer is a three-dimensional (3D) structure consisting of neighboring cells, extracellular matrix, and blood vessels. It is therefore critical to mimic the cancer cells and their surrounding environment duringin vitrostudy. Our aim was to establish a 3D cancer model using a synthetic composite scaffold.Methods. High-density low-volume seeding was used to promote attachment of a non-small-cell lung cancer cell line (NCI-H460) to scaffolds. Growth patterns in 3D culture were compared with those of monolayers. Immunohistochemistry was conducted to compare the expression of Ki67, CD44, and carbonic anhydrase IX.Results. NCI-H460 readily attached to the scaffold without surface pretreatment at a rate of 35% from a load of 1.5 × 106cells. Most cells grew vertically to form clumps along the surface of the scaffold, and cell morphology resembled tissue origin; 2D cultures exhibited characteristics of adherent epithelial cancer cell lines. Expression patterns of Ki67, CD44, and CA IX varied markedly between 3D and monolayer cultures.Conclusions. The behavior of cancer cells in our 3D model is similar to tumor growthin vivo. This model will provide the basis for future study using 3D cancer culture.

Fabrication and characterization of electrospinning/3D printing bone tissue engineering scaffold

RSC Advances ◽  
2016 ◽  
Vol 6 (112) ◽  
pp. 110557-110565 ◽  
Author(s):  
Yinxian Yu ◽  
Sha Hua ◽  
Mengkai Yang ◽  
Zeze Fu ◽  
Songsong Teng ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Tissue Engineering ◽  
3D Printing ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Composite Scaffold ◽  
Tissue Engineering Scaffold ◽  
Fabrication And Characterization

A composite scaffold was fabricated with a method involving both electrospinning and 3D printing to give microscale pores and good mechanical properties. Biocompatibility and cell infiltration on the scaffold was evaluated by an in vitro study.

Synthesis and In Vitro Characterization of Carbon Nano Tube-Polycaprolactone Composite Scaffold for Odontoblast Cell Interaction

2016 ◽  
Vol 720 ◽  
pp. 114-119
Author(s):  
Mohamad Ali Ketabi ◽  
Maryam Shahnavazi ◽  
Reza Fekrazad ◽  
Farbod Tondnevis ◽  
Hamid Keshvari ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Composite Scaffold ◽  
Complex Structure ◽  
Cell Interaction ◽  
Dental Tissue ◽  
Nano Fiber ◽  
Dental Tissue Engineering ◽  
Carbon Nano Tube

Dentin tissue engineering is one of innovative and effective regenerative method for treatment of dental pulp and dental tissue, and our research concentrated on preparing an effective scaffold substrate containing carbon Nano tube in polycaprolactone Nano fiber matrix. Our characterization showed benefits of this complex structure and its effect on odontoblast cell interaction. The results indicated that the prepared nanofiber polymer contains 4% CNT have a great potential as biocompatible materials for use in dental tissue engineering and odontoblast cell interaction.

scholarly journals Comparative study of T84 and T84SF human colon carcinoma cells: in vitro and in vivo ultrastructural and functional characterization of cell culture and metastasis

2006 ◽  
Vol 449 (1) ◽  
pp. 48-61
Author(s):  
Riccardo Alessandro ◽  
Maria Antonietta Di Bella ◽  
Anna Maria Flugy ◽  
Simona Fontana ◽  
Francesca Damiani ◽  
...  
Keyword(s):  
Cell Culture ◽  
Comparative Study ◽  
Functional Characterization ◽  
Human Colon ◽  
Carcinoma Cells ◽  
Colon Carcinoma Cells ◽  
Human Colon Carcinoma

scholarly journals ACP-TX-I and ACP-TX-II, Two Novel Phospholipases A2 Isolated from Trans-Pecos Copperhead Agkistrodon contortrix pictigaster Venom: Biochemical and Functional Characterization

Toxins ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 661 ◽  
Author(s):  
Salomón Huancahuire-Vega ◽  
Luciana M. Hollanda ◽  
Mauricio Gomes-Heleno ◽  
Edda E. Newball-Noriega ◽  
Sergio Marangoni
Keyword(s):  
Functional Characterization ◽  
In Vitro Cytotoxicity ◽  
Carcinoma Cells ◽  
Phospholipases A2 ◽  
Agkistrodon Contortrix ◽  
Lung Carcinoma Cells ◽  
A549 Lung

This work reports the purification and biochemical and functional characterization of ACP-TX-I and ACP-TX-II, two phospholipases A2 (PLA2) from Agkistrodon contortrix pictigaster venom. Both PLA2s were highly purified by a single chromatographic step on a C18 reverse phase HPLC column. Various peptide sequences from these two toxins showed similarity to those of other PLA2 toxins from viperid snake venoms. ACP-TX-I belongs to the catalytically inactive K49 PLA2 class, while ACP-TX-II is a D49 PLA2, and is enzymatically active. ACP-TX-I PLA2 is monomeric, which results in markedly diminished myotoxic and inflammatory activities when compared with dimeric K49 PLA2s, confirming the hypothesis that dimeric structure contributes heavily to the profound myotoxicity of the most active viperid K49 PLA2s. ACP-TX-II exhibits the main pharmacological actions reported for this protein family, including in vivo local myotoxicity, edema-forming activity, and in vitro cytotoxicity. ACP-TX-I PLA2 is cytotoxic to A549 lung carcinoma cells, indicating that cytotoxicity to these tumor cells does not require enzymatic activity.

In Vitro and In Vivo Characterization of Various Ferrites for Hyperthermia in Cancer-Treatment

2005 ◽  
Vol 284-286 ◽  
pp. 827-830
Author(s):  
D.H. Kim ◽  
Se Ho Lee ◽  
Kyoung Nam Kim ◽  
Kwang Mahn Kim ◽  
I.B. Shim ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Cancer Treatment ◽  
Carcinoma Cells ◽  
Alternating Magnetic Field ◽  
Normal Cells ◽  
Tumor Bearing ◽  
In Vivo Characterization

Ceramic ferrites can be used to cancer-treatment. Heating of certain organs or tissue up to temperature between 42oC and 45oC preferentially for cancer therapy is called hyperthermia. We synthesized ferrites with various compositions in the system Co1-xNixFe2O4 as hyperthermic thermoseed in cancer-treatment and evaluated their effects on the necrosis of cancer cells under alternating magnetic field in vivo as well as in vitro. When a CoFe2O4 was placed into 0.2 ml distilled water, the greatest temperature change in this study, Δ T=29.3oC, was observed. More than half of the carcinoma cells were dead after exposure to alternating magnetic field using CoFe2O4, while normal cells were survived more than 60%. The injection of this ferrite particles into the tumor bearing mice was able to suppress the number and volume of tumors. CoFe2O4 is expected the useful hyperthermic thermoseed in cancer-treatment because it exhibited the greatest necrosis of carcinoma cells in vitro and in vivo.

Fate of antibody-neocarzinostatin conjugates bound to human colonic carcinoma cells in vitro

1992 ◽  
Vol 28 (9-10) ◽  
pp. 569-570 ◽  
Author(s):  
Takashi Yokota ◽  
Kazuya Kitamura ◽  
Toshiharu Yamaguchi ◽  
Toshio Takahashi
Keyword(s):  
Carcinoma Cells ◽  
Colonic Carcinoma

In vitro generation of tumour-specific lymphocyte reactivity to colonic carcinoma cells

1987 ◽  
Vol 25 (3) ◽  
Author(s):  
Maria Kuppner ◽  
Stephen Wilkinson ◽  
Elizabeth Casson ◽  
Oleg Eremin
Keyword(s):  
Carcinoma Cells ◽  
Colonic Carcinoma ◽  
Lymphocyte Reactivity
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