scholarly journals Autophagy Inhibition Sensitizes Renal Tubular Epithelial Cell to G1 Arrest Induced by Transforming Growth Factor beta (TGF-β)

2020 ◽  
Vol 26 ◽  
Author(s):  
Chen Yang ◽  
Hong-luan Wu ◽  
Zhi-hang Li ◽  
Xiao-cui Chen ◽  
Hong-yong Su ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epithelial Cell ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Tubular Epithelial Cell ◽  
G1 Arrest ◽  
Renal Tubular Epithelial Cell ◽  
Growth Factor Beta ◽  
Renal Tubular ◽  
Autophagy Inhibition

Mechanisms of recovery from mechanical injury of renal tubular epithelial cells

1994 ◽  
Vol 267 (2) ◽  
pp. F257-F264 ◽  
Author(s):  
H. T. Sponsel ◽  
R. Breckon ◽  
W. Hammond ◽  
R. J. Anderson
Keyword(s):  
Wound Healing ◽  
Growth Factor ◽  
Epithelial Cell ◽  
Transforming Growth Factor ◽  
Mdck Cells ◽  
Tubular Epithelial Cell ◽  
Thymidine Uptake ◽  
Tgf Beta ◽  
Renal Tubular Epithelial Cell ◽  
Renal Tubular

The mechanism(s) whereby a denuded renal tubular epithelial cell surface becomes reestablished remains unknown. We therefore measured the rate of renewal of mechanical wounds made in confluent monolayers of two established renal tubular epithelial cell lines. We found that wounds of MDCK cells heal at a faster rate than wounds of LLC-PK1 cells. The magnitude of wound healing did not differ when cells grown on plastic were compared with cells grown on fibronectin, laminin, or collagen. Irradiation (4,000 rads) of MDCK and LLC-PK1 cells significantly reduced indexes of proliferation (5-bromo-2'-deoxyuridine and thymidine uptake) without affecting wound healing. Serum and epidermal growth factor (EGF) enhance whereas transforming growth factor-beta 1 (TGF-beta 1) impairs wound healing. Hepatocyte growth factor (HGF) stimulates wound healing at low concentrations and inhibits healing at high concentrations in MDCK cells while not affecting healing of LLC-PK1 cell wounds at any concentration. Several interleukins (IL-1, IL-2, IL-3, and IL-6) did not affect wound healing in either cell type. Healing of LLC-PK1 but not MDCK cells was impaired by exposure to a peptide containing a RGD sequence. Conversely, healing of MDCK but not LLC-PK1 cells was impaired by the REDV tetrapeptide. Healing of both LLC-PK1 and MDCK was impaired by heparin but not by the LDVPS peptide. These results demonstrate that mechanical wounds of LLC-PK1 and MDCK cells heal, at least in part, by migration. Healing is regulated by serum and growth factors including EGF, HGF, and TGF-beta 1.(ABSTRACT TRUNCATED AT 250 WORDS)

The Effects of Cyclosporine on Protein Expression of P53 and Transforming Growth Factor-Beta in Human Renal Tubular Cells

2000 ◽  
Vol 6 (S2) ◽  
pp. 604-605
Author(s):  
H. Song ◽  
C. Wei
Keyword(s):  
Growth Factor ◽  
Cyclosporine A ◽  
Transforming Growth Factor Beta ◽  
Renal Cell ◽  
Transforming Growth Factor ◽  
Tubular Cells ◽  
Renal Tubular Cells ◽  
Cellular Apoptosis ◽  
Growth Factor Beta ◽  
Renal Tubular

Cyclosporine-A (CsA) is the widely used immunosuppressant drug in renal transplantation. However, the effects of cyclosporine-A are limited by a significant nephrotoxicity. The mechanisms of CsA-induced allograft nephropathy are remaining controversial. Recent study indicated that cellular apoptosis may contribute to the cyclosporine A-mediated cytotoxic action. To date, regarding the effects of cyclosporine A on renal cell apoptosis-related gene expression remain poorly defined. p53 is an important gene in control of renal cell growth and death. Transforming growth factor-beta (TGF-beta) is a multifunctional cytokine that has anti-proliferative as well as fibrogenic properties.We hypothesized that cyclosporine-A may increase p53 and TGF-β expression in renal tubular cells. These actions of cyclosporine-A may contribute to the cellular apoptosis, fibrosis and CsA-induced nephrotoxicity. Therefore, current study was designed to determine the effects of cyclosporine-A on the p53 and TGF-βl protein expression by immunohistochemical staining (IHCS) in cultured human tubular cells.

scholarly journals Increased renal tubular expression of transforming growth factor beta in human allografts correlates with cyclosporine toxicity

1996 ◽  
Vol 50 (5) ◽  
pp. 1634-1640 ◽  
Author(s):  
Oleh G. Pankewycz ◽  
Li Miao ◽  
Ross Isaacs ◽  
Jing Guan ◽  
Tim Pruett ◽  
...  
Keyword(s):  
Growth Factor ◽  
Transforming Growth Factor Beta ◽  
Transforming Growth Factor ◽  
Growth Factor Beta ◽  
Renal Tubular
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