scholarly journals Identification of Differentially Expressed Genes (DEGs) Relevant to Prognosis of Ovarian Cancer by Use of Integrated Bioinformatics Analysis and Validation by Immunohistochemistry Assay

2019 ◽  
Vol 25 ◽  
pp. 9902-9912
Author(s):  
Limin Zhang ◽  
Lijun Sun ◽  
Bin Zhang ◽  
Lihong Chen
Keyword(s):  
Ovarian Cancer ◽  
Differentially Expressed Genes ◽  
Bioinformatics Analysis ◽  
Differentially Expressed

Bioinformatics analysis on differentially expressed genes of alveolar macrophage in IPF

2019 ◽  
Vol 45 (9-10) ◽  
pp. 288-296 ◽  
Author(s):  
Huaibin Wang ◽  
Miaomiao Wang ◽  
Kun Xiao ◽  
Xu Zhang ◽  
Peng Wang ◽  
...  
Keyword(s):  
Alveolar Macrophage ◽  
Differentially Expressed Genes ◽  
Bioinformatics Analysis ◽  
Differentially Expressed

scholarly journals Bioinformatic gene analysis for potential therapeutic targets of Huntington’s disease in pre-symptomatic and symptomatic stage

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Chunchen Xiang ◽  
Shengri Cong ◽  
Bin Liang ◽  
Shuyan Cong
Keyword(s):  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Differentially Expressed Genes ◽  
Bioinformatics Analysis ◽  
Neurodegenerative Disorder ◽  
Psychiatric Symptoms ◽  
Therapeutic Targets ◽  
Differentially Expressed ◽  
Hub Genes ◽  
Transcriptional Dysregulation

Abstract Background Huntington’s disease (HD) is a neurodegenerative disorder characterized by psychiatric symptoms, serious motor and cognitive deficits. Certain pathological changes can already be observed in pre-symptomatic HD (pre-HD) patients; however, the underlying molecular pathogenesis is still uncertain and no effective treatments are available until now. Here, we reanalyzed HD-related differentially expressed genes from the GEO database between symptomatic HD patients, pre-HD individuals, and healthy controls using bioinformatics analysis, hoping to get more insight in the pathogenesis of both pre-HD and HD, and shed a light in the potential therapeutic targets of the disease. Methods Pre-HD and symptomatic HD differentially expressed genes (DEGs) were screened by bioinformatics analysis Gene Expression Omnibus (GEO) dataset GSE1751. A protein–protein interaction (PPI) network was used to select hub genes. Subsequently, Gene Ontology (GO) enrichment analysis of DEGs and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of hub genes were applied. Dataset GSE24250 was downloaded to verify our hub genes by the Kaplan–Meier method using Graphpad Prism 5.0. Finally, target miRNAs of intersected hub genes involved in pre-HD and symptomatic HD were predicted. Results A total of 37 and 985 DEGs were identified in pre-HD and symptomatic HD, respectively. The hub genes, SIRT1, SUZ12, and PSMC6, may be implicated in pre-HD, and the hub genes, FIS1, SIRT1, CCNH, SUZ12, and 10 others, may be implicated in symptomatic HD. The intersected hub genes, SIRT1 and SUZ12, and their predicted target miRNAs, in particular miR-22-3p and miR-19b, may be significantly associated with pre-HD. Conclusion The PSMC6, SIRT1, and SUZ12 genes and their related ubiquitin-mediated proteolysis, transcriptional dysregulation, and histone metabolism are significantly associated with pre-HD. FIS1, CCNH, and their related mitochondrial disruption and transcriptional dysregulation processes are related to symptomatic HD, which might shed a light on the elucidation of potential therapeutic targets in HD.

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