scholarly journals Cerebellar Fastigial Nucleus Stimulation in a Chronic Unpredictable Mild Stress Rat Model Reduces Post-Stroke Depression by Suppressing Brain Inflammation via the microRNA-29c/TNFRSF1A Signaling Pathway

2019 ◽  
Vol 25 ◽  
pp. 5594-5605 ◽  
Author(s):  
Mu Wang ◽  
Jian Guo ◽  
Li-na Dong ◽  
Jun-Ping Wang
Keyword(s):  
Signaling Pathway ◽  
Rat Model ◽  
Brain Inflammation ◽  
Fastigial Nucleus ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Post Stroke ◽  
Post Stroke Depression

scholarly journals Helicid Reverses the Effect of Overexpressing NCALD, Which Blocks the sGC/cGMP/PKG Signaling Pathway in the CUMS-Induced Rat Model

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Xiao-Tong Zhang ◽  
Yuan Zhang ◽  
Yuan-Xiang Zhang ◽  
Zhen-Yi Jiang ◽  
Hui Yang ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Western Blotting ◽  
Rat Model ◽  
Caspase 3 ◽  
Underlying Mechanism ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Adeno Associated Virus ◽  
Protein Levels ◽  
Antidepressant Effects

Background. Increasing evidence has shown that apoptosis in the hippocampus is closely related to depressive-like behavior. We previously reported that helicid had good antidepressant activities, which manifested as the alleviation of depression-like behaviors and the reversal of the high expression of neurocalcin delta (NCALD) in chronic unpredictable mild stress (CUMS) rats. The aim of this study was, therefore, to characterize the antidepressant-like effects and underlying mechanism of helicid on CUMS rats by silencing NCALD and using rescue experiments. Methods. We developed the CUMS rat model using CUMS stimulation from week 0 to week 6. The rats were treated with helicid, or NCALD silenced, then we overexpressed NCALD using adeno-associated virus. We also measured the protein levels of sGCα1, sGCβ1, PKG1/2, and cleaved caspase-3 in hippocampal tissues using western blotting and measured cGMP using an ELISA. Results. Treating CUMS rats by silencing NCALD or by the administration of helicid improved the depressive-like behavior. The levels of proteins, including sGC, PKG, cleaved caspase-3, and cGMP, in hippocampus all decreased. NCALD overexpression reversed these decreases and reversed the alleviation of depression-like behaviors in CUMS rats. Limitation. We only detected the antidepressant effects of helicid in the hippocampus; therefore, other parts of brain should also be studied. Conclusions. Inhibition of NCALD, as well as helicid administration, alleviated antidepressant-like behavior by regulating the expressions of apoptotic cytokines and the sGC/cGMP/PKG signaling pathway. Overexpressing NCALD reversed the amelioration effects of silenced NCALD and helicid administration.

scholarly journals Cerebellar Fastigial Nucleus Electrical Stimulation Alleviates Depressive-Like Behaviors in Post-Stroke Depression Rat Model and Potential Mechanisms

2017 ◽  
Vol 41 (4) ◽  
pp. 1403-1412 ◽  
Author(s):  
Lei Zhang ◽  
Mingkui Zhao ◽  
Ru-Bo Sui
Keyword(s):  
Weight Loss ◽  
Electrical Stimulation ◽  
Purkinje Cells ◽  
Inflammatory Cytokines ◽  
Rat Model ◽  
Mrna Level ◽  
Fastigial Nucleus ◽  
Post Stroke ◽  
Post Stroke Depression ◽  
Cerebellar Purkinje Cells

Objective: To identify the molecular mechanism of post-stroke depression (PSD), and observe the therapeutic effects of cerebellar fastigial nucleus electrical stimulation (FNS) on the behaviors and regional cerebral blood flow (rCBF) in a PSD rat model. Methods: Healthy SD rats were randomly divided into four groups (sham, stroke, post-stroke depress and FNS group). Sham group (n = 6) underwent sham operation. The other three groups (n = 6*3) underwent MCAO. Rats were examined twice a week in open filed test. Moreover, neuroprotective effect on cerebellar Purkinje cells and expression of cytokines in hippocampal tissue were examined. Results: The PSD group showed a significant weight loss, decreased consumption of sucrose water, reduced rearing and locomotor activities. The FNS significantly alleviates the body weight loss and sucrose preference, locomotor and rearing activities. The bilateral rCBF was also restored after FNS treatment. Moreover, FNS improved the neuroprotection via suppressing apoptosis of cerebellar Purkinje cells. And the inflammatory cytokines mRNA level in hippocampus was significantly decreased. Conclusion: FNS treatment alleviates depressive-like behaviors and rCBF in PSD rats model, which could be attributed to its ability to protect cerebellar Purkinje cells and decrease the mRNA level of inflammatory cytokines.

A metabolomics-based approach for ranking the depressive level in a chronic unpredictable mild stress rat model

RSC Advances ◽  
2016 ◽  
Vol 6 (31) ◽  
pp. 25751-25765 ◽  
Author(s):  
Xinyu Yu ◽  
Shanlei Qiao ◽  
Di Wang ◽  
Jiayong Dai ◽  
Jun Wang ◽  
...  
Keyword(s):  
Animal Model ◽  
Major Depressive Disorder ◽  
Prefrontal Cortex ◽  
Depressive Disorder ◽  
Rat Model ◽  
Untargeted Metabolomics ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress ◽  
Major Depressive ◽  
Metabolomics Study

An untargeted metabolomics study to investigate the metabolome change in plasma, hippocampus and prefrontal cortex (PFC) in an animal model with a major depressive disorder (MDD) had been conducted.

Antidepressant-like effects of Xiaochaihutang in a rat model of chronic unpredictable mild stress

2014 ◽  
Vol 152 (1) ◽  
pp. 217-226 ◽  
Author(s):  
Guang Yue Su ◽  
Jing Yu Yang ◽  
Fang Wang ◽  
Jie Ma ◽  
Kuo Zhang ◽  
...  
Keyword(s):  
Rat Model ◽  
Mild Stress ◽  
Chronic Unpredictable Mild Stress
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