scholarly journals Proteomic Profile in Glomeruli of Type-2 Diabetic KKAy Mice using 2-Dimensional Differential Gel Electrophoresis

2014 ◽  
Vol 20 ◽  
pp. 2705-2713 ◽  
Author(s):  
Lining Wang
Keyword(s):  
Gel Electrophoresis ◽  
Proteomic Profile ◽  
Kkay Mice ◽  
Differential Gel Electrophoresis ◽  
Type 2 Diabetic

scholarly journals Stachyose improves the anti-diabetic effects of berberine by regulating intestinal microbiota and SCFAs in spontaneous type 2 diabetic KKAy mice

2020 ◽  
Author(s):  
Hui Cao ◽  
Cai-Na Li ◽  
Lei Lei ◽  
Xing Wang ◽  
Shuai-Nan Liu ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Blood Glucose Control ◽  
Intestinal Barrier ◽  
Short Chain Fatty Acids ◽  
Oral Glucose ◽  
Beneficial Effects ◽  
Combination Treatments ◽  
Kkay Mice ◽  
Type 2 Diabetic

Abstract Background: Berberine (BBR) has the beneficial effects of anti-inflammation, anti-bacteria, and anti-diabetes. The clinical application of BBR has been hindered by its poor gastrointestinal absorption. Stachyose (Sta), a prebiotic agent, improves the composition of gut microbiota and benefits for diabetes. We therefore investigated whether Sta improves the anti-diabetic actions of BBR using type 2 diabetic KKAy mice.Methods: KKAy mice were randomly divided into four groups: Con, Sta (200 mg/kg), BBR (100 mg/kg), and BBR+Sta (BBR: 100 mg/kg, Sta: 200 mg/kg). Mice were administered intragastrically once daily for 8 weeks. The mice weight, glycemia, glycated hemoglobin, and levels of insulin, glucagon, and inflammatory cytokines were monitored. Oral glucose tolerance tests and insulin tolerance tests were performed; protein and gene expression levels were detected by Western blot and qRT-PCR, respectively; gut microbiota and short-chain fatty acids (SCFAs) in feces were analyzed.Results: The combination of BBR and Sta is more effective than BBR alone in blood glucose control, improvement of insulin resistance and islet functions, inflammatory mediators decrease, and maintenance of intestinal barrier integrity. Gut microbiota analysis demonstrates that both BBR and combination treatments enhance the abundance of Bacteroidaceae and Akkermansiaceae and decrease Lachnospiraceae levels, whereas Akkermansiaceae elevation due to the administration of BBR with Sta is more significant than BBR alone. Interestingly, the proportion of Lactobacillaceae increases with combination treatment, but is diminished by BBR treatment. Additionally, BBR with Sta significantly reduces the concentrations of fecal SCFAs compared to BBR.Conclusions: The combination of BBR and Sta imparts better effects on the maintenance of glycemia and intestinal homeostasis than BBR alone by modulating gut microbiota and SCFAs, thereby providing a novel approach for the treatment of type 2 diabetes mellitus.

scholarly journals Hypoglycemic Action of Chicken Meat Extract in Type-2 Diabetic KKAy Mice and GK Rats

2009 ◽  
Vol 73 (12) ◽  
pp. 2583-2588 ◽  
Author(s):  
Meng-Kwoon SIM ◽  
Yong-Chiat WONG ◽  
Xiao-Guang XU ◽  
Sai-Zhen SIM ◽  
Daniel TSI
Keyword(s):  
Chicken Meat ◽  
Meat Extract ◽  
Gk Rats ◽  
Kkay Mice ◽  
Hypoglycemic Action ◽  
Type 2 Diabetic

scholarly journals A novel SIRT1 activator E6155 improves insulin sensitivity in type 2 diabetic KKAy mice

2018 ◽  
Vol 498 (3) ◽  
pp. 633-639 ◽  
Author(s):  
Peng Liu ◽  
Tingting Feng ◽  
Xuan Zuo ◽  
Xiao Wang ◽  
Jinque Luo ◽  
...  
Keyword(s):  
Insulin Sensitivity ◽  
Kkay Mice ◽  
Type 2 Diabetic ◽  
Sirt1 Activator

scholarly journals Add-On Aliskiren Elicits Stronger Renoprotection Than High-Dose Valsartan in Type 2 Diabetic KKAy Mice That Do Not Respond to Low-Dose Valsartan

2012 ◽  
Vol 119 (2) ◽  
pp. 131-138 ◽  
Author(s):  
Bai Lei ◽  
Daisuke Nakano ◽  
Yu-Yan Fan ◽  
Kento Kitada ◽  
Hirofumi Hitomi ◽  
...  
Keyword(s):  
Low Dose ◽  
High Dose ◽  
Kkay Mice ◽  
Type 2 Diabetic
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