scholarly journals Evidence of a Large Diversity of N-acyl-Homoserine Lactones in Symbiotic Vibrio fischeri Strains Associated with the Squid Euprymna scolopes

2019 ◽  
Vol 34 (1) ◽  
pp. 99-103 ◽  
Author(s):  
Léa Girard ◽  
Elodie Blanchet ◽  
Didier Stien ◽  
Julia Baudart ◽  
Marcelino Suzuki ◽  
...  
Keyword(s):  
Vibrio Fischeri ◽  
Euprymna Scolopes ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

scholarly journals Human Paraoxonase-2 (PON2): Protein Functions and Modulation

Antioxidants ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 256
Author(s):  
Giuseppe Manco ◽  
Elena Porzio ◽  
Teresa Maria Carusone
Keyword(s):  
Hydrolytic Activity ◽  
Principal Function ◽  
Substrate Specificities ◽  
Homoserine Lactones ◽  
Stress Control ◽  
Acyl Homoserine Lactones ◽  
Bacterial Quorum Sensing ◽  
Protein Functions ◽  
Bacterial Quorum ◽  
Hydrolyzing Enzymes

PON1, PON2, and PON3 belong to a family of lactone hydrolyzing enzymes endowed with various substrate specificities. Among PONs, PON2 shows the highest hydrolytic activity toward many acyl-homoserine lactones (acyl-HL) involved in bacterial quorum-sensing signaling. Accordingly, defense against pathogens, such as Brevundimonas aeruginosa (B. aeruginosa), was postulated to be the principal function of PON2. However, recent findings have highlighted the importance of PON2 in oxidative stress control, inhibition of apoptosis, and the progression of various types of malignancies. This review focuses on all of these aspects of PON2.

scholarly journals Sampling, separation, and quantification of N ‐acyl homoserine lactones from marine intertidal sediments

2021 ◽  
Vol 19 (2) ◽  
pp. 145-157
Author(s):  
Frederike Stock ◽  
Emilio Cirri ◽  
Samarasinghe Gunasekara Liyanage Is Nuwanthi ◽  
Willem Stock ◽  
Nico Ueberschaar ◽  
...  
Keyword(s):  
Intertidal Sediments ◽  
Homoserine Lactones ◽  
Acyl Homoserine Lactones

scholarly journals FlrA, a σ54-Dependent Transcriptional Activator in Vibrio fischeri, Is Required for Motility and Symbiotic Light-Organ Colonization

2003 ◽  
Vol 185 (12) ◽  
pp. 3547-3557 ◽  
Author(s):  
Deborah S. Millikan ◽  
Edward G. Ruby
Keyword(s):  
Vibrio Fischeri ◽  
Transcriptional Activator ◽  
Pcr Analysis ◽  
Euprymna Scolopes ◽  
Wild Type ◽  
Flagellar Regulon ◽  
Colonization Factors ◽  
Recognition Motifs ◽  
Arbitrarily Primed Pcr ◽  
Organ Colonization

ABSTRACT Flagellum-mediated motility of Vibrio fischeri is an essential factor in the bacterium's ability to colonize its host, the Hawaiian squid Euprymna scolopes. To begin characterizing the nature of the flagellar regulon, we have cloned a gene, designated flrA, from V. fischeri that encodes a putative σ54-dependent transcriptional activator. Genetic arrangement of the flrA locus in V. fischeri is similar to motility master-regulator operons of Vibrio cholerae and Vibrio parahaemolyticus. In addition, examination of regulatory regions of a number of flagellar operons in V. fischeri revealed apparent σ54 recognition motifs, suggesting that the flagellar regulatory hierarchy is controlled by a similar mechanism to that described in V. cholerae. However, in contrast to its closest known relatives, flrA mutant strains of V. fischeri ES114 were completely abolished in swimming capability. Although flrA provided in trans restored motility to the flrA mutant, the complemented strain was unable to reach wild-type levels of symbiotic colonization in juvenile squid, suggesting a possible role for the proper expression of FlrA in regulating symbiotic colonization factors in addition to those required for motility. Comparative RNA arbitrarily primed PCR analysis of the flrA mutant and its wild-type parent revealed several differentially expressed transcripts. These results define a regulon that includes both flagellar structural genes and other genes apparently not involved in flagellum elaboration or function. Thus, the transcriptional activator FlrA plays an essential role in regulating motility, and apparently in modulating other symbiotic functions, in V. fischeri.

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