scholarly journals Phylogenetic Analyses of Bacterial Communities Developed in a Cassette-Electrode Microbial Fuel Cell

2009 ◽  
Vol 24 (2) ◽  
pp. 188-192 ◽  
Author(s):  
Takefumi Shimoyama ◽  
Akira Yamazawa ◽  
Yoshiyuki Ueno ◽  
Kazuya Watanabe
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Bacterial Communities ◽  
Phylogenetic Analyses

Azospirillum humicireducens sp. nov., a nitrogen-fixing bacterium isolated from a microbial fuel cell

2013 ◽  
Vol 63 (Pt_7) ◽  
pp. 2618-2624 ◽  
Author(s):  
Shungui Zhou ◽  
Luchao Han ◽  
Yueqiang Wang ◽  
Guiqin Yang ◽  
Li Zhuang ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Type Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Nitrogen Fixing ◽  
Content Type ◽  
Link Type ◽  
Type Strains

A Gram-negative, facultative anaerobic, motile, spiral, straight-to-slightly curved rod-shaped and nitrogen-fixing strain, designated SgZ-5T, was isolated from a microbial fuel cell (MFC) and was characterized by means of a polyphasic approach. Growth occurred with 0–1 % (w/v) NaCl (optimum 1 %) and at pH 5.5–8.5 (optimum pH 7.2) and at 25–37 °C (optimum 30 °C) in nutrient broth (NB). The strain had the ability to grow under anaerobic conditions via the oxidation of various organic compounds coupled to the reduction of anthraquione-2,6-disulfonate (AQDS). Chemotaxonomic characteristics (main ubiquinone Q-10, major fatty acid C18 : 1ω7c/C18 : 1ω6c and DNA G+C content 67.7 mol%) were similar to those of members of the genus Azospirillum . According to the results of phylogenetic analyses, strain SgZ-5T belonged to the genus Azospirillum within the family Rhodospirillaceae of the class Alphaproteobacteria , and was related most closely to the type strains of Azospirillum lipoferum , Azospirillum thiophilum and Azospirillum oryzae (98.0, 97.6 and 97.1 % 16S rRNA gene sequence similarity, respectively). DNA–DNA pairing studies showed that the unidentified organism displayed reassociation values of 36.7±3.7, 24.1±2.2 and 22.3±2.4 % to the type strains of A. lipoferum , A. thiophilum and A. oryzae , respectively. Similarities between nifH gene sequences of strain SgZ-5T and members of the genus Azospirillum ranged from 94.0 to 97.0 %. A combination of phenotypic, chemotaxonomic, phylogenetic and genotypic data clearly indicated that strain SgZ-5T represents a novel species, for which the name Azospirillum humicireducens sp. nov. is proposed. The type strain is SgZ-5T ( = CCTCC AB 2012021T = KACC 16605T).

Electrochemical and phylogenetic analyses of current-generating microorganisms in a thermophilic microbial fuel cell

2013 ◽  
Vol 115 (3) ◽  
pp. 268-271 ◽  
Author(s):  
Qian Fu ◽  
Hajime Kobayashi ◽  
Hideo Kawaguchi ◽  
Javier Vilcaez ◽  
Tatsuki Wakayama ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Phylogenetic Analyses

scholarly journals Ureibacillus defluvii sp. nov., isolated from a thermophilic microbial fuel cell

2014 ◽  
Vol 64 (Pt_5) ◽  
pp. 1617-1621 ◽  
Author(s):  
Shungui Zhou ◽  
Jia Tang ◽  
Dongxing Qin ◽  
Qin Lu ◽  
Guiqin Yang
Keyword(s):  
Fuel Cell ◽  
16S Rrna ◽  
Microbial Fuel Cell ◽  
Type Species ◽  
Novel Species ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type

A thermophilic bacterium, designated DX-1T, was isolated from the anode biofilm of a microbial fuel cell (MFC). Cells of strain DX-1T were oxidase-positive, catalase-positive and Gram-staining-negative. The strain was found to be rod-shaped and non-motile and to produce subterminal spores. The strain was able to grow with NaCl at concentrations ranging from 0 to 6 %, at temperatures of 25–60 °C (optimum 55 °C) and pH 6.0–8.0 (optimum pH 7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain DX-1T formed a cluster with Ureibacillus thermosphaericus DSM 10633T (96.9 % 16S rRNA sequence similarity), Ureibacillus composti DSM 17951T (95.8 %), Ureibacillus thermophilus DSM 17952T (95.7 %) and Ureibacillus terrenus DSM 12654T (95.3 %). The G+C content of the genomic DNA was 40.4 mol%. The major quinone was MK-7, the peptidoglycan type was l-Lys←d-Asp, and the major cellular fatty acids (>5 %) were iso-C16 : 0 and iso-C14 : 0. The polar lipids consisted of phosphatidylglycerol, diphosphatidylglycerol and phospholipids of unknown composition. Based on phenotypic characteristics, chemotaxonomic features and results of phylogenetic analyses, the strain was determined to represent a distinct novel species of the genus Ureibacillus , and the name proposed for the novel species is Ureibacillus defluvii sp. nov., with type strain DX-1T ( = CGMCC 1.12358T = KCTC 33127T).

Bacterial Communities on Electron-Beam Pt-Deposited Electrodes in a Mediator-Less Microbial Fuel Cell

2008 ◽  
Vol 42 (16) ◽  
pp. 6243-6249 ◽  
Author(s):  
Ho Il Park ◽  
David Sanchez ◽  
Sung Kwon Cho ◽  
Minhee Yun
Keyword(s):  
Electron Beam ◽  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Bacterial Communities

Electricity production and bacterial communities of microbial fuel cell supplied with oily sludge

2020 ◽  
Vol 39 (5) ◽  
Author(s):  
Haiying Guo ◽  
Shuixiang Xie ◽  
Hao Deng ◽  
Xiaoheng Geng ◽  
Penghua Wang ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Bacterial Communities ◽  
Electricity Production ◽  
Oily Sludge

Fontibacter ferrireducens sp. nov., an Fe(III)-reducing bacterium isolated from a microbial fuel cell

2013 ◽  
Vol 63 (Pt_3) ◽  
pp. 925-929 ◽  
Author(s):  
Jun Zhang ◽  
GuiQin Yang ◽  
Shungui Zhou ◽  
Yueqiang Wang ◽  
Yong Yuan ◽  
...  
Keyword(s):  
Fuel Cell ◽  
Microbial Fuel Cell ◽  
Type Species ◽  
Iron Reduction ◽  
Sequence Similarity ◽  
Phylogenetic Analyses ◽  
Distinct Species ◽  
Respiratory Quinone ◽  
Content Type ◽  
Link Type

A novel, dissimilatory Fe(III)-reducing bacterium, designated strain SgZ-2T, which could couple glucose oxidation to iron reduction for energy conservation, was isolated from a microbial fuel cell. The isolate was Gram-staining-negative, catalase-positive, oxidase-negative and facultatively anaerobic. The strain was able to grow on tryptic soy agar at 15–37 °C and in the presence of 0–5 % (w/v) NaCl. The predominant cellular fatty acids (>5 %) were iso-C15 : 0, iso-C17 : 0 3-OH and iso-C17 : 1ω9c; the major respiratory quinone was MK-7; the major polar lipids were phosphatidylethanolamine, an unidentified aminolipid and three other unidentified lipids; and the DNA G+C content was 55.3 mol%. Phylogenetic analyses based on 16S rRNA sequences showed that the novel strain was most closely related to Fontibacter flavus CC-GZM-130T (99.9 % sequence similarity), and was a member of the family Cyclobacteriaceae . The levels of DNA–DNA relatedness observed between strain SgZ-2T and F. flavus CCM 7650T (<41 %) indicated that the two strains represented two distinct species. Based on phylogenetic analyses and phenotypic characteristics, strain SgZ-2T represents a novel species of the genus Fontibacter , for which the name Fontibacter ferrireducens sp. nov. is proposed. The type strain is SgZ-2T ( = CCTCC M 2011498T  = KACC 16525T).

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