Isolation and Characterization of Long-Chain-Alkane Degrading Bacillus thermoleovorans from Deep Subterranean Petroleum Reservoirs.

2001 ◽  
Vol 91 (1) ◽  
pp. 64-70 ◽  
Author(s):  
TOMOHISA KATO ◽  
MITSURU HARUKI ◽  
TADAYUKI IMANAKA ◽  
MASAAKI MORIKAWA ◽  
SHIGENORI KANAYA
Peptides ◽  
2016 ◽  
Vol 80 ◽  
pp. 9-17 ◽  
Author(s):  
Felipe Augusto Cerni ◽  
Manuela Berto Pucca ◽  
Fernanda Gobbi Amorim ◽  
Karla de Castro Figueiredo Bordon ◽  
Julien Echterbille ◽  
...  

2002 ◽  
Vol 30 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
L. V. Michaelson ◽  
A. J. Longman ◽  
O. Sayanova ◽  
A. K. Stobart ◽  
J. A. Napier

We have isolated a cDNA encoding the Δ8 sphingolipid desaturase from the plant Aquilegia vulgaris L. via a PCR-based strategy using primers designed to target the conserved histidine box regions of microsomal desaturases. The function of the cDNA was confirmed by expression in the yeast, Saccharomyces cerevisiae. Analysis of the long-chain sphingoid bases as their dinitrophenyl derivatives by reverse-phase HPLC demonstrated the accumulation of cis- and trans-desaturated sphingoid bases which were not present in the wild-type yeast cells. The Δ8 desaturated products co-eluted with known Δ8-desaturated phytosphingenine and the molecular mass of these products was confirmed by liquid chromatography-MS. The Δ8 long-chain base desaturase was also able to desaturate dihydrosphingosine substrates. This is the first report of the functional characterization of an A. vulgaris gene product.


RSC Advances ◽  
2018 ◽  
Vol 8 (73) ◽  
pp. 41966-41975 ◽  
Author(s):  
Ying Li ◽  
Ting Cui ◽  
Yaxin Wang ◽  
Xizhen Ge

Kitchen waste oil is composed of long chain triglycerides (LCTs) that has high energy density.


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