Combination of poly(ethylenimine) and chitosan induces high gene transfection efficiency and low cytotoxicity

2008 ◽  
Vol 105 (1) ◽  
pp. 65-68 ◽  
Author(s):  
Qing-Qing Zhao ◽  
Jin-Liang Chen ◽  
Min Han ◽  
Wen-Quan Liang ◽  
Yasuhiko Tabata ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
High Gene ◽  
Low Cytotoxicity

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

2016 ◽  
Vol 4 (39) ◽  
pp. 6462-6467 ◽  
Author(s):  
Sheng-Gang Ding ◽  
Lei Yu ◽  
Long-Hai Wang ◽  
Lin-Ding Wang ◽  
Zhi-Qiang Yu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Dendritic Polymer ◽  
Self Assembling ◽  
High Gene ◽  
Low Cytotoxicity

Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery.

PGMA-based gene carriers with lipid molecules

2016 ◽  
Vol 4 (8) ◽  
pp. 1233-1243 ◽  
Author(s):  
Chen Xu ◽  
Bingran Yu ◽  
Hao Hu ◽  
Muhammad Naeem Nizam ◽  
Wei Yuan ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Tumor Treatment ◽  
Gene Carriers ◽  
High Gene ◽  
Low Cytotoxicity

A series of effective cationic conjugations of lipid molecules with low cytotoxicity and high gene transfection efficiency were readily designed for gene therapy and tumor treatment.

A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites

Biomaterials ◽  
2014 ◽  
Vol 35 (28) ◽  
pp. 8261-8272 ◽  
Author(s):  
Raviraj Vankayala ◽  
Chi-Shiun Chiang ◽  
Jui-I. Chao ◽  
Chiun-Jye Yuan ◽  
Shyr-Yeu Lin ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
General Strategy ◽  
Lipid Nanoparticle ◽  
High Gene ◽  
Nanoparticle Composites

Gene-carried chitosan-linked-PEI induced high gene transfection efficiency with low toxicity and significant tumor-suppressive activity

2010 ◽  
Vol 387 (1-2) ◽  
pp. 286-294 ◽  
Author(s):  
Jian-Qing Gao ◽  
Qing-Qing Zhao ◽  
Teng-Fei Lv ◽  
Wu-Ping Shuai ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Suppressive Activity ◽  
High Gene ◽  
Low Toxicity

Cationic niosomes composed of spermine-based cationic lipids mediate high gene transfection efficiency

2012 ◽  
Vol 20 (9) ◽  
pp. 783-792 ◽  
Author(s):  
Orapan Paecharoenchai ◽  
Nattisa Niyomtham ◽  
Tanasait Ngawhirunpat ◽  
Theerasak Rojanarata ◽  
Boon-ek Yingyongnarongkul ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Cationic Lipids ◽  
High Gene ◽  
Cationic Niosomes

Gene-carried chitosan-linked polyethylenimine induced high gene transfection efficiency on dendritic cells

2012 ◽  
Vol 59 (5) ◽  
pp. 346-352 ◽  
Author(s):  
Yu-Zhe Chen ◽  
Xing-Lei Yao ◽  
Gui-Xin Ruan ◽  
Qing-Qing Zhao ◽  
Gu-Ping Tang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
High Gene

Efficient gene transfection to liver cells via the cellular regulation of a multifunctional polylactitol-based gene transporter

2016 ◽  
Vol 4 (12) ◽  
pp. 2208-2218 ◽  
Author(s):  
Young-Dong Kim ◽  
Tae-Eun Park ◽  
Bijay Singh ◽  
Kye-Soo Cho ◽  
Jaiprakash N. Sangshetti ◽  
...  
Keyword(s):  
Liver Cell ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Gene Carrier ◽  
Cell Targeting ◽  
Cellular Regulation ◽  
Efficient Gene ◽  
Low Cytotoxicity

A new polylactitol-based multifunctional gene carrier has shown low cytotoxicity, a high transfection efficiency, and liver cell targeting bothin vitroandin vivo.

scholarly journals Ar-23 Derivatives With High Endosomal Disrupting Ability Enhance Poly(L-lysine)-mediated Gene Transfection

2020 ◽  
Author(s):  
Shikun Zhang ◽  
Lin Gong ◽  
Xue Zhang ◽  
Zhi-min Yun ◽  
Su-Bo Li ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Physicochemical Characteristics ◽  
Cationic Polymer ◽  
Ph Sensitive ◽  
Lytic Activity ◽  
Endosomal Escape ◽  
Charged Residues ◽  
Low Cytotoxicity ◽  
Positively Charged

Abstract Background：pH-sensitive peptides are a relatively new strategy for conquering poor endosomal release of cationic polymer-mediated transfection. Modification of antimicrobial peptides (AMPs) by exchanging positively-charged residues with negatively-charged glutamic acid residues (Glu) greatly improved its lytic activity at the endosomal pH, which could improve cationic polymer-mediated transfection.Methods：In the present study, we investigate the effect of the number of Glu substituted for positively-charged residues on the endosomal escape activity of AR-23 and the ability of mutated AR-23 for enhancing cationic polymer-mediated transfection. Three analogs were synthesized by replacing the positively-charged residues in AR-23's sequence with Glu one-by-one. The pH-sensitive lysis ability of the peptides, the effect of peptides on the physicochemical characteristics, the intracellular trafficking，the transfection efficiency, and the cytotoxicity of the polyplexes were determined.Results：Increased lytic activity of peptides was observed with the increased number of Glu replacement in the AR-23 sequence at acidic pH. The number of Glu substituted for positively-charged residues of AR-23 dramatically affects its lysis ability at neutral pH. Triple-Glu substitution in AR-23's sequence greatly improved PLL-mediated gene transfection efficiency while maintaining low cytotoxicity.Conclusion：The results indicate that replacement of positively-charged residues with enough Glu residues may be considered a method for designing pH-sensitive peptides, which could be applied as potential enhancers for improving cationic polymer-mediated transfection.

Continuous Vector-free Gene Transfer with a Novel Microfluidic Chip and Nanoneedle Array

2018 ◽  
Vol 16 (2) ◽  
pp. 164-170 ◽  
Author(s):  
Dong Huang ◽  
Deyao Zhao ◽  
Jinhui Li ◽  
Yuting Wu ◽  
Lili Du ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Basic Research ◽  
Nanoneedle Array ◽  
Contact Frequency ◽  
Delivery Platform ◽  
High Gene ◽  
Free Gene

Background: Delivery of foreign cargoes into cells is of great value for bioengineering research and therapeutic applications. Objective: In this study, we proposed and established a carrier-free gene delivery platform utilizing staggered herringbone channel and silicon nanoneedle array, to achieve high-throughput in vitro gene transfection. </P><P> Methods: With this microchip, fluidic micro vortices could be induced by the staggered-herringboneshaped grooves within the channel, which increased the contact frequency of the cells with the channel substrate. Transient disruptions on the cell membrane were well established by the nanoneedle array on the substrate. </P><P> Result: Compared to the conventional nanoneedle-based delivery system, proposed microfluidic chip achieved flow-through treatment with high gene transfection efficiency (higher than 20%) and ideal cell viability (higher than 95%). It provides a continuous processing environment that can satisfy the transfection requirement of large amounts of biological molecules, showing high potential and promising prospect for both basic research and clinical application.

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