Gene-carried chitosan-linked polyethylenimine induced high gene transfection efficiency on dendritic cells

2012 ◽  
Vol 59 (5) ◽  
pp. 346-352 ◽  
Author(s):  
Yu-Zhe Chen ◽  
Xing-Lei Yao ◽  
Gui-Xin Ruan ◽  
Qing-Qing Zhao ◽  
Gu-Ping Tang ◽  
...  
Keyword(s):  
Dendritic Cells ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
High Gene

A general strategy to achieve ultra-high gene transfection efficiency using lipid-nanoparticle composites

Biomaterials ◽  
2014 ◽  
Vol 35 (28) ◽  
pp. 8261-8272 ◽  
Author(s):  
Raviraj Vankayala ◽  
Chi-Shiun Chiang ◽  
Jui-I. Chao ◽  
Chiun-Jye Yuan ◽  
Shyr-Yeu Lin ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
General Strategy ◽  
Lipid Nanoparticle ◽  
High Gene ◽  
Nanoparticle Composites

Self-assembling Janus dendritic polymer for gene delivery with low cytotoxicity and high gene transfection efficiency

2016 ◽  
Vol 4 (39) ◽  
pp. 6462-6467 ◽  
Author(s):  
Sheng-Gang Ding ◽  
Lei Yu ◽  
Long-Hai Wang ◽  
Lin-Ding Wang ◽  
Zhi-Qiang Yu ◽  
...  
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Dendritic Polymer ◽  
Self Assembling ◽  
High Gene ◽  
Low Cytotoxicity

Polycations have high DNA condensing ability, low immunogenicity, and great adaptability, which make them promising for gene delivery.

Gene-carried chitosan-linked-PEI induced high gene transfection efficiency with low toxicity and significant tumor-suppressive activity

2010 ◽  
Vol 387 (1-2) ◽  
pp. 286-294 ◽  
Author(s):  
Jian-Qing Gao ◽  
Qing-Qing Zhao ◽  
Teng-Fei Lv ◽  
Wu-Ping Shuai ◽  
Jun Zhou ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Suppressive Activity ◽  
High Gene ◽  
Low Toxicity

Cationic niosomes composed of spermine-based cationic lipids mediate high gene transfection efficiency

2012 ◽  
Vol 20 (9) ◽  
pp. 783-792 ◽  
Author(s):  
Orapan Paecharoenchai ◽  
Nattisa Niyomtham ◽  
Tanasait Ngawhirunpat ◽  
Theerasak Rojanarata ◽  
Boon-ek Yingyongnarongkul ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Cationic Lipids ◽  
High Gene ◽  
Cationic Niosomes

Continuous Vector-free Gene Transfer with a Novel Microfluidic Chip and Nanoneedle Array

2018 ◽  
Vol 16 (2) ◽  
pp. 164-170 ◽  
Author(s):  
Dong Huang ◽  
Deyao Zhao ◽  
Jinhui Li ◽  
Yuting Wu ◽  
Lili Du ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Basic Research ◽  
Nanoneedle Array ◽  
Contact Frequency ◽  
Delivery Platform ◽  
High Gene ◽  
Free Gene

Background: Delivery of foreign cargoes into cells is of great value for bioengineering research and therapeutic applications. Objective: In this study, we proposed and established a carrier-free gene delivery platform utilizing staggered herringbone channel and silicon nanoneedle array, to achieve high-throughput in vitro gene transfection. </P><P> Methods: With this microchip, fluidic micro vortices could be induced by the staggered-herringboneshaped grooves within the channel, which increased the contact frequency of the cells with the channel substrate. Transient disruptions on the cell membrane were well established by the nanoneedle array on the substrate. </P><P> Result: Compared to the conventional nanoneedle-based delivery system, proposed microfluidic chip achieved flow-through treatment with high gene transfection efficiency (higher than 20%) and ideal cell viability (higher than 95%). It provides a continuous processing environment that can satisfy the transfection requirement of large amounts of biological molecules, showing high potential and promising prospect for both basic research and clinical application.

Galactosylated ternary DNA/polyphosphoramidate nanoparticles mediate high gene transfection efficiency in hepatocytes

2005 ◽  
Vol 102 (3) ◽  
pp. 749-763 ◽  
Author(s):  
Xue-Qing Zhang ◽  
Xu-Li Wang ◽  
Peng-Chi Zhang ◽  
Zhi-Lan Liu ◽  
Ren-Xi Zhuo ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
High Gene

Well-Defined Poly(2-hydroxyl-3-(2-hydroxyethylamino)propyl methacrylate) Vectors with Low Toxicity and High Gene Transfection Efficiency

2010 ◽  
Vol 11 (6) ◽  
pp. 1437-1442 ◽  
Author(s):  
F. J. Xu ◽  
M. Y. Chai ◽  
W. B. Li ◽  
Y. Ping ◽  
G. P. Tang ◽  
...  
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Propyl Methacrylate ◽  
High Gene ◽  
Low Toxicity

PLL/PAE/DNA ternary complexes with enhanced endosomal escape ability for efficient and safe gene transfection

2016 ◽  
Vol 40 (11) ◽  
pp. 9806-9812 ◽  
Author(s):  
Zhibing Sun ◽  
Dezhong Zhou
Keyword(s):  
Transfection Efficiency ◽  
Gene Transfection ◽  
Ternary Complexes ◽  
Endosomal Escape ◽  
High Gene

High gene transfection efficiency is achieved by a simple yet versatile PAE oligomer incorporation strategy.

scholarly journals Aminated β-Cyclodextrin-Modified-Carboxylated Magnetic Cobalt/Nanocellulose Composite for Tumor-Targeted Gene Delivery

2014 ◽  
Vol 2014 ◽  
pp. 1-10 ◽  
Author(s):  
Thayyath Sreenivasan Anirudhan ◽  
Sylaja Raveendran Rejeena
Keyword(s):  
Gene Delivery ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Luciferase Expression ◽  
High Dose ◽  
Targeted Gene Delivery ◽  
Life Threatening ◽  
High Gene ◽  
Good Biocompatibility ◽  
Potential Biodegradability

Gene therapy is a new kind of medicine, which uses genes as drugs in order to treat life threatening diseases. In the present work, a nonviral vector, aminated β-cyclodextrin-modified-carboxylated magnetic cobalt/nanocellulose composite (ACDC-Co/NCC), was synthesized for efficient transfection of genes into tumour cells. The synthesized ACDC-Co/NCC was characterized by means of FTIR, XRD, SEM, and ESR techniques. DNA condensing ability of ACDC-Co/NCC was found to be increased with increase in amount of ACDC-Co/NCC and 84.9% of DNA (1.0 μg/mL) inclusion was observed with 6.0 μg/mL of ACDC-Co/NCC. The cytotoxicity of ACDC-Co/NCC was observed to be minimal, even at higher concentration, with respect to the model transfecting agent, poly(ethyleneimine) (PEI). 88.2% of the gene was transfected at high dose of DNA, as indicated by the highest luciferase expression. These results indicated that ACDC-Co/NCC might be a promising candidate for gene delivery with the characteristics of good biocompatibility, potential biodegradability, minimal cytotoxicity, and relatively high gene transfection efficiency.

Gene transfection efficiency into dendritic cells is influenced by the size of cationic liposomes/DNA complexes

2017 ◽  
Vol 102 ◽  
pp. 230-236 ◽  
Author(s):  
Yoshikazu Inoh ◽  
Mie Nagai ◽  
Kayo Matsushita ◽  
Mamoru Nakanishi ◽  
Tadahide Furuno
Keyword(s):  
Dendritic Cells ◽  
Transfection Efficiency ◽  
Gene Transfection ◽  
Cationic Liposomes ◽  
Dna Complexes
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